国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
14期
2046-2048
,共3页
铁甲草%肝损伤%D-氨基半乳糖%ALT%AST
鐵甲草%肝損傷%D-氨基半乳糖%ALT%AST
철갑초%간손상%D-안기반유당%ALT%AST
Cassia mimosoides%Liver injury%D-galactosamine%ALT%AST
目的 观察铁甲草急性毒性反应;观察铁甲草水煎液作用于肝损伤小鼠后,对ALT、AST水平的影响.方法 铁甲草给予最大剂量,观察药物急性毒性反应;将小鼠随机分为高、低剂量组、阳性对照组、模型对照组和正常对照组,除了正常对照组,各组腹腔注射D-GalN造肝损伤模型后,各组连续给药5天,每天1次,末次给药1h后,取血清测定ALT和AST水平.结果 小鼠最大耐受剂量为150 g/kg,相当于临床用药的500倍,在该剂量下,动物一般状态良好,未见明显毒性反应症状;铁甲草水煎液高剂量组与模型组比较,ALT、AST水平差异有统计学意义,虽然铁甲草低剂量组与模型组ALT、AST值在统计学上没有显著性差异,但有减低的趋势.结论 小鼠最大耐受剂量下,没有明显急性毒性作用;铁甲草水煎液能降低肝损伤小鼠ALT、AST水平,提示铁甲草水煎液对肝损伤具有一定的保护作用,高剂量组作用明显优于低剂量组,说明了保护作用与剂量有关,作用机制有待于进一步深入探讨和研究.
目的 觀察鐵甲草急性毒性反應;觀察鐵甲草水煎液作用于肝損傷小鼠後,對ALT、AST水平的影響.方法 鐵甲草給予最大劑量,觀察藥物急性毒性反應;將小鼠隨機分為高、低劑量組、暘性對照組、模型對照組和正常對照組,除瞭正常對照組,各組腹腔註射D-GalN造肝損傷模型後,各組連續給藥5天,每天1次,末次給藥1h後,取血清測定ALT和AST水平.結果 小鼠最大耐受劑量為150 g/kg,相噹于臨床用藥的500倍,在該劑量下,動物一般狀態良好,未見明顯毒性反應癥狀;鐵甲草水煎液高劑量組與模型組比較,ALT、AST水平差異有統計學意義,雖然鐵甲草低劑量組與模型組ALT、AST值在統計學上沒有顯著性差異,但有減低的趨勢.結論 小鼠最大耐受劑量下,沒有明顯急性毒性作用;鐵甲草水煎液能降低肝損傷小鼠ALT、AST水平,提示鐵甲草水煎液對肝損傷具有一定的保護作用,高劑量組作用明顯優于低劑量組,說明瞭保護作用與劑量有關,作用機製有待于進一步深入探討和研究.
목적 관찰철갑초급성독성반응;관찰철갑초수전액작용우간손상소서후,대ALT、AST수평적영향.방법 철갑초급여최대제량,관찰약물급성독성반응;장소서수궤분위고、저제량조、양성대조조、모형대조조화정상대조조,제료정상대조조,각조복강주사D-GalN조간손상모형후,각조련속급약5천,매천1차,말차급약1h후,취혈청측정ALT화AST수평.결과 소서최대내수제량위150 g/kg,상당우림상용약적500배,재해제량하,동물일반상태량호,미견명현독성반응증상;철갑초수전액고제량조여모형조비교,ALT、AST수평차이유통계학의의,수연철갑초저제량조여모형조ALT、AST치재통계학상몰유현저성차이,단유감저적추세.결론 소서최대내수제량하,몰유명현급성독성작용;철갑초수전액능강저간손상소서ALT、AST수평,제시철갑초수전액대간손상구유일정적보호작용,고제량조작용명현우우저제량조,설명료보호작용여제량유관,작용궤제유대우진일보심입탐토화연구.
Objective To observe the acute toxicity reaction of Cassia mimosoides and to observe the influence of Cassia mimosoides decoction on the levels of ALT and AST of liver injury mice.Methods Cassia mimosoides was given a maximum dose to observe the acute toxicity reaction of drugs.The mice were randomly divided into high and low dose groups,a positive control group,a model control group and a normal control group.Besides the normal control group,D-GalN was intraperitoneally injected in all the other groups to make liver injury models.Continuous administration lasted for 5 days,1 times per day.1 hour after the last administration,the serum ALT and AST levels were determined.Results The maximum tolerance dose of mice was 150 g/kg,equivalent to 500 times of clinical medicine; the animal were generally in good condition in this dose; no obvious symptoms of toxicity was found.There were statistical differences in AST and ALT levels between the high dose group and the model group.Though there were no statistical differences in AST and ALT levels between the low dose group and the model group,there was a downward trend in AST an ALT levels.Conclusions Mice taking a dose less than maximum tolerance dose has no acute toxicity.Cassia mimosoides decoction can decrease the levels of AST and ALT of liver injury mice,suggesting that Cassia mimosoides decoction has a protective effect on liver injury.High dose group was superior to low dose group,showing that the protective effect increases with dose.Its mechanisms need to be further explored and studied.
