国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2012年
6期
264-269
,共6页
李奇凤%张斌%柳彩霞%韩艳%杜爽%余亮%贾娜尔%阿依代%段招军%孙荷
李奇鳳%張斌%柳綵霞%韓豔%杜爽%餘亮%賈娜爾%阿依代%段招軍%孫荷
리기봉%장빈%류채하%한염%두상%여량%가나이%아의대%단초군%손하
人博卡病毒%呼吸道感染%儿童%巢氏PCR
人博卡病毒%呼吸道感染%兒童%巢氏PCR
인박잡병독%호흡도감염%인동%소씨PCR
Human bocavirus%Respiratory tract infection%Children%Nested PCR
目的 了解新疆乌鲁木齐地区住院呼吸道感染患儿中人博卡病毒(human bocavirus,HBoV)的检出情况.方法 收集新疆维吾尔自治区人民医院2010年1月至2011年12月住院呼吸道感染患儿鼻咽抽吸物标本525例,用巢氏PCR扩增人HBoV NS1片段检测HBoV 1 ～4型.结果 在525份标本中,HBoV总阳性检出率为8.38％ (44/525),其中HBoV 1型6例,HBoV 2型38例,未检出HBoV 3、4型.各民族患儿之间HBoV阳性检出率统计分析发现,维族、回族患几分别高于汉族患儿,差异有统计学意义(P＜0.05).HBoV 1型与参考株核苷酸序列相似度为97.7％ ～ 99.4％,HBoV2型与参考株核苷酸序列相似度为93.6～ 100％.HBoV的检出时间主要以冬春季为主,HBoV感染在年龄及性别方面差异均无统计学意义.结论 2010～ 2011年乌鲁木齐地区呼吸道感染的住院患儿存在HBoV 1、2型的流行,以HBoV 2型为主,维族、回族患儿HBoV的检出率分别高于汉族患儿.
目的 瞭解新疆烏魯木齊地區住院呼吸道感染患兒中人博卡病毒(human bocavirus,HBoV)的檢齣情況.方法 收集新疆維吾爾自治區人民醫院2010年1月至2011年12月住院呼吸道感染患兒鼻嚥抽吸物標本525例,用巢氏PCR擴增人HBoV NS1片段檢測HBoV 1 ～4型.結果 在525份標本中,HBoV總暘性檢齣率為8.38％ (44/525),其中HBoV 1型6例,HBoV 2型38例,未檢齣HBoV 3、4型.各民族患兒之間HBoV暘性檢齣率統計分析髮現,維族、迴族患幾分彆高于漢族患兒,差異有統計學意義(P＜0.05).HBoV 1型與參攷株覈苷痠序列相似度為97.7％ ～ 99.4％,HBoV2型與參攷株覈苷痠序列相似度為93.6～ 100％.HBoV的檢齣時間主要以鼕春季為主,HBoV感染在年齡及性彆方麵差異均無統計學意義.結論 2010～ 2011年烏魯木齊地區呼吸道感染的住院患兒存在HBoV 1、2型的流行,以HBoV 2型為主,維族、迴族患兒HBoV的檢齣率分彆高于漢族患兒.
목적 료해신강오로목제지구주원호흡도감염환인중인박잡병독(human bocavirus,HBoV)적검출정황.방법 수집신강유오이자치구인민의원2010년1월지2011년12월주원호흡도감염환인비인추흡물표본525례,용소씨PCR확증인HBoV NS1편단검측HBoV 1 ～4형.결과 재525빈표본중,HBoV총양성검출솔위8.38％ (44/525),기중HBoV 1형6례,HBoV 2형38례,미검출HBoV 3、4형.각민족환인지간HBoV양성검출솔통계분석발현,유족、회족환궤분별고우한족환인,차이유통계학의의(P＜0.05).HBoV 1형여삼고주핵감산서렬상사도위97.7％ ～ 99.4％,HBoV2형여삼고주핵감산서렬상사도위93.6～ 100％.HBoV적검출시간주요이동춘계위주,HBoV감염재년령급성별방면차이균무통계학의의.결론 2010～ 2011년오로목제지구호흡도감염적주원환인존재HBoV 1、2형적류행,이HBoV 2형위주,유족、회족환인HBoV적검출솔분별고우한족환인.
Objective To study human bocavirus (HBoV) infections in hospitalized children with respiratory tract infection in Urumqi area.Methods Nasopharyngeal aspirates (NPA) samples were collected from 525 hospitalized children with respiratory tract infection at People's Hospital of Xinjiang Uygur Autonomous Region from 2010 to 2011.Nested PCR was used to amplify NS1 fragments to screen HBoV 1 to HBoV 4.Results The overall frequency of HBoVs was 8.38％ (44/525),including six cases of HBoV 1,thirty-eight cases of HBoV 2.HBoV 3 and HBoV 4 were not detected.HBoVs positive rates of Uygur and Hui children were statistically significantly higher than Han respectively (P ＜0.05).Nucleotide sequences of six strains of HBoV 1 were 97.7％ ～ 99.4％ identical with that of 3 reference strains.Thirty-eight strains of HBoV 2 had 93.6 ～ 100％ identical nucleotide sequence with 3 reference strains.HBoVs mainly occurred in winter and spring.No statistically significant differences in sex and ages were found.Conclusions HBoV 1 and HBoV 2 were circulating among hospitalized children with respiratory tract infection from 2010 to 2011 in Urumqi area with HBoV 2 as the dominant subtype.HBoVs positive rates of Uygur and Hui children were higher than Han respectively.
