国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2013年
3期
118-122
,共5页
张海龙%李苑%姚相杰%冼慧霞%杨洪%罗敏%何雅青
張海龍%李苑%姚相傑%冼慧霞%楊洪%囉敏%何雅青
장해룡%리원%요상걸%승혜하%양홍%라민%하아청
腹泻%诺如病毒%分子流行病学%进化树
腹瀉%諾如病毒%分子流行病學%進化樹
복사%낙여병독%분자류행병학%진화수
Diarrhea%Norovirus%Molecular epidemiology%Phylogenetic tree
目的 了解深圳市宝安区诺如病毒腹泻的分子流行病学特点.方法 采集深圳市宝安区2010年1月至2011年12月486例疑似病毒性腹泻患者粪便标本,使用商品化的GⅡ型诺如病毒荧光PCR试剂盒检测GⅡ型诺如病毒核酸.随机挑选若干GⅡ型诺如病毒阳性标本,用GⅡ型诺如病毒特异性引物COG2F/G2-SKR进行RT-PCR扩增,其他所有阴性标本用GⅠ型诺如病毒特异性引物GI-SKF/GI-SKR进行RT-PCR扩增.阳性产物回收纯化并测序,用Clustal W和MEGA 5.0生物软件对诺如病毒序列进行序列比对和系统进化分析.结果 48份随机挑选的GⅡ型诺如病毒阳性标本中检出GⅡ.4/2006b型36株,GⅡ.4/2008型3株,GⅡ.5型9株;无诺如病毒GⅠ型检出.结论 GⅡ 型是2010年1月至2011年12月深圳市宝安区诺如病毒的主要型别,其中GⅡ.4/2006b是最重要的流行株.
目的 瞭解深圳市寶安區諾如病毒腹瀉的分子流行病學特點.方法 採集深圳市寶安區2010年1月至2011年12月486例疑似病毒性腹瀉患者糞便標本,使用商品化的GⅡ型諾如病毒熒光PCR試劑盒檢測GⅡ型諾如病毒覈痠.隨機挑選若榦GⅡ型諾如病毒暘性標本,用GⅡ型諾如病毒特異性引物COG2F/G2-SKR進行RT-PCR擴增,其他所有陰性標本用GⅠ型諾如病毒特異性引物GI-SKF/GI-SKR進行RT-PCR擴增.暘性產物迴收純化併測序,用Clustal W和MEGA 5.0生物軟件對諾如病毒序列進行序列比對和繫統進化分析.結果 48份隨機挑選的GⅡ型諾如病毒暘性標本中檢齣GⅡ.4/2006b型36株,GⅡ.4/2008型3株,GⅡ.5型9株;無諾如病毒GⅠ型檢齣.結論 GⅡ 型是2010年1月至2011年12月深圳市寶安區諾如病毒的主要型彆,其中GⅡ.4/2006b是最重要的流行株.
목적 료해심수시보안구낙여병독복사적분자류행병학특점.방법 채집심수시보안구2010년1월지2011년12월486례의사병독성복사환자분편표본,사용상품화적GⅡ형낙여병독형광PCR시제합검측GⅡ형낙여병독핵산.수궤도선약간GⅡ형낙여병독양성표본,용GⅡ형낙여병독특이성인물COG2F/G2-SKR진행RT-PCR확증,기타소유음성표본용GⅠ형낙여병독특이성인물GI-SKF/GI-SKR진행RT-PCR확증.양성산물회수순화병측서,용Clustal W화MEGA 5.0생물연건대낙여병독서렬진행서렬비대화계통진화분석.결과 48빈수궤도선적GⅡ형낙여병독양성표본중검출GⅡ.4/2006b형36주,GⅡ.4/2008형3주,GⅡ.5형9주;무낙여병독GⅠ형검출.결론 GⅡ 형시2010년1월지2011년12월심수시보안구낙여병독적주요형별,기중GⅡ.4/2006b시최중요적류행주.
Objective To investigate the molecular characteristics of noroviruses among viral diarrhea cases in Baoan district,Shenzhen.Methods From January 2010 to December 2011,486 stool samples were collected from suspected viral diarrhea patients in Baoan district,Shenzhen.Nucleic acid of GⅡ noroviruses were detected using real-time polymerase chain reaction (real-time PCR).The positive samples for GⅡ norovirus were randomly selected to perform reverse transcription-polymerase chain reaction (RTPCR) with the primers COG2F/G2-SKR,while all the negative samples for GⅡ norovirus were performed RT-PCR with the primers GI-SKF/GI-SKR.Positive PCR products were purified and sequenced,and the sequences were analyzed by Clustal W and MEGA 5.0 programs,then phylogenetic tree was constructed and genotype was identified.Results Among 48 positive samples for GⅡ norovirus,the numbers of G Ⅱ.4/ 2006b,G Ⅱ.4/2008 and GⅡ.5 were 36,3,and 9 respectively; No GⅠ norovirus was found.Conclusions From January 2010 to December 2011,G Ⅱ was the most important genotype of norovirus,and G Ⅱ.4/2006b variant was identified as the predominant strain in Baoan district,Shenzhen.