CAJ | 학술논문

目的探讨病毒性心肌炎患者外周血单核细胞来源树突状细胞(DCs)的功能,为治疗病毒性心肌炎提供一种新的途径.方法选取病毒性心肌炎患者与身体健康、状态良好的志愿者各20例分别作为病例组与对照组,采集研究对象外周血单核细胞进行无菌化分离,再分别给予粒细胞巨噬细胞集落刺激因子(GM-CSF)和白细胞介素4(IL-4)诱导外周血Mo-DCs,观察其表面形状变化、吞噬能力的变化以及其表达细胞因子的结果.结果病例组外周血单核细胞Mo-DCs中的CD80、CD83、CD86及MHC Ⅱ表达水平高于对照组,但Mo-DCs吞噬功能低于对照组.与对照组比较,病例组患者细胞TNF-a较高(P＜0.05)和IL-12(P＜ 0.05).结论病毒性心肌炎患者外周血单核细胞Mo-DCs处于一个十分成熟稳定的功能状态,可表达较高水平的IL-12和TNF-12.
목적 탐토병독성심기염환자외주혈단핵세포래원수돌상세포(DCs)적공능,위치료병독성심기염제공일충신적도경.방법 선취병독성심기염환자여신체건강、상태량호적지원자각20례분별작위병례조여대조조,채집연구대상외주혈단핵세포진행무균화분리,재분별급여립세포거서세포집락자격인자(GM-CSF)화백세포개소4(IL-4)유도외주혈Mo-DCs,관찰기표면형상변화、탄서능력적변화이급기표체세포인자적결과.결과 병례조외주혈단핵세포Mo-DCs중적CD80、CD83、CD86급MHC Ⅱ표체수평고우대조조,단Mo-DCs탄서공능저우대조조.여대조조비교,병례조환자세포TNF-a교고(P＜0.05)화IL-12(P＜ 0.05).결론 병독성심기염환자외주혈단핵세포Mo-DCs처우일개십분성숙은정적공능상태,가표체교고수평적IL-12화TNF-12.
Objective To explore the function of peripheral blood monocyte-derived DCs of patients with viral myocarditis,to develop a new approach for the treatment of viral myocarditis.Methods A total of 20 viral myocarditis patients and 20 healthy volunteers were selected as cases and controls,respectively.The peripheral blood mononuclear cells were collected and separated firstly,and then they were induced by GMCSF and IL-4.We observed the changes of shape and phagocytic ability of the cells,as well as the expression of cytokines.Results The expression levels of CD80,CD83,CD86,and MHC Ⅱ were higher in cases than that in controls.However,the phagocytosis of Mo-DCs in cases was lower.Besides,Mo-DCs of cases secreted higher level of TNF-a (P＜ 0.05) and IL-12 (P＜ 0.05).Conclusions The Mo-DCs of viral myocarditis patients' are very mature and stable.They have a high level expression of TNF-12 and IL-12.