国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2014年
2期
66-70
,共5页
任莉娜%杜溪乔%王建%王宇%张炳丽%宋晓琳
任莉娜%杜溪喬%王建%王宇%張炳麗%宋曉琳
임리나%두계교%왕건%왕우%장병려%송효림
A组轮状病毒%G1型%VP7基因%变异
A組輪狀病毒%G1型%VP7基因%變異
A조륜상병독%G1형%VP7기인%변이
Group A rotavirus%G1 type%VP7 gene%Variation
目的 了解哈尔滨地区G1型A组轮状病毒VP7基因变异特点.方法 选取2007-2012年哈尔滨地区33株G1型A组轮状病毒标本,采用半巢氏PCR进行G1型鉴定,并对VP7基因进行克隆测序.采用DNASTAR软件对序列进行分析及进化树的绘制.结果 33株哈尔滨地方株VP7基因均为1062个碱基,推导氨基酸序列同源性95.7％～100％;33株哈尔滨地方株根据进化树分为四簇,其中Ⅰ簇17株,Ⅱ簇12株,Ⅲ簇、Ⅳ簇各2株.在氨基酸水平上,Ⅰ簇与其他三簇的整体差异位点为aa75(V→I),Ⅱ簇哈尔滨地方株变异比较活跃,Ⅱ簇、Ⅲ簇哈尔滨地方株具有一定的中国地方性,Ⅳ簇参考株均来自东南亚地区;2007年的哈尔滨地方株VP7蛋白变异位点最多,2008年次之,2010年-2012年哈尔滨地方株主要集中在Ⅰ簇.结论 33株轮状病毒G1型哈尔滨地方株可分为四个分支,Ⅰ簇是优势分支,且随着监测年份的变化Ⅰ簇所占构成比逐渐增加.
目的 瞭解哈爾濱地區G1型A組輪狀病毒VP7基因變異特點.方法 選取2007-2012年哈爾濱地區33株G1型A組輪狀病毒標本,採用半巢氏PCR進行G1型鑒定,併對VP7基因進行剋隆測序.採用DNASTAR軟件對序列進行分析及進化樹的繪製.結果 33株哈爾濱地方株VP7基因均為1062箇堿基,推導氨基痠序列同源性95.7％～100％;33株哈爾濱地方株根據進化樹分為四簇,其中Ⅰ簇17株,Ⅱ簇12株,Ⅲ簇、Ⅳ簇各2株.在氨基痠水平上,Ⅰ簇與其他三簇的整體差異位點為aa75(V→I),Ⅱ簇哈爾濱地方株變異比較活躍,Ⅱ簇、Ⅲ簇哈爾濱地方株具有一定的中國地方性,Ⅳ簇參攷株均來自東南亞地區;2007年的哈爾濱地方株VP7蛋白變異位點最多,2008年次之,2010年-2012年哈爾濱地方株主要集中在Ⅰ簇.結論 33株輪狀病毒G1型哈爾濱地方株可分為四箇分支,Ⅰ簇是優勢分支,且隨著鑑測年份的變化Ⅰ簇所佔構成比逐漸增加.
목적 료해합이빈지구G1형A조륜상병독VP7기인변이특점.방법 선취2007-2012년합이빈지구33주G1형A조륜상병독표본,채용반소씨PCR진행G1형감정,병대VP7기인진행극륭측서.채용DNASTAR연건대서렬진행분석급진화수적회제.결과 33주합이빈지방주VP7기인균위1062개감기,추도안기산서렬동원성95.7％～100％;33주합이빈지방주근거진화수분위사족,기중Ⅰ족17주,Ⅱ족12주,Ⅲ족、Ⅳ족각2주.재안기산수평상,Ⅰ족여기타삼족적정체차이위점위aa75(V→I),Ⅱ족합이빈지방주변이비교활약,Ⅱ족、Ⅲ족합이빈지방주구유일정적중국지방성,Ⅳ족삼고주균래자동남아지구;2007년적합이빈지방주VP7단백변이위점최다,2008년차지,2010년-2012년합이빈지방주주요집중재Ⅰ족.결론 33주륜상병독G1형합이빈지방주가분위사개분지,Ⅰ족시우세분지,차수착감측년빈적변화Ⅰ족소점구성비축점증가.
Objective In order to understand the diversity of the VP7 genes among group A rotavirus G1 type strains in Harbin.Methods Thirty-three rotavirus strains of G1 type were selected in Harbin during 2007-2012.G1 type was identified using the semi-nested PCR,and the VP7 genes were cloned and sequenced.The phylogenetic tree was constructed by DNASTAR software.Results The VP7 genes of all 33 strains were 1062 bp in size,and the amino acid identity was 95.7％ ～ 100％ among themselves.Phylogenetic analysis revealed that 33 Harbin strains were divided into four clusters,17 strains belonged to cluster Ⅰ,12 of cluster Ⅱ,2 of cluster Ⅲ,and 2 of Ⅳ.At the amino acid level,compared with the other three clusters,mutations of aa75 (V→I) were found in all strains of Cluster Ⅰ.More mutated sites were detected in Cluster Ⅱ,and clusters Ⅱ and Ⅲ were often seen in our country.Cluster Ⅳ were highly homologous with the reference strains circulating in southeast Asia.Most mutated sites of VP7 protein among Harbin local strains were found in 2007,followed by 2008,the Harbin local strains mainly belonged to cluster Ⅰ during 2010 to 2012.Conclusions Thirty-three Harbin strains of rotavirus G1 type were divided into four clusters by phylogenetic analysis,among which cluster Ⅰ was predominant,and the proportion of cluster Ⅰ was increased year by year.
