国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2014年
2期
71-75
,共5页
张海龙%李苑%赵德坚%刘滕颖子%冼慧霞%杨洪%罗敏%姚相杰%何雅青
張海龍%李苑%趙德堅%劉滕穎子%冼慧霞%楊洪%囉敏%姚相傑%何雅青
장해룡%리원%조덕견%류등영자%승혜하%양홍%라민%요상걸%하아청
肠道腺病毒%分子流行病学%基因进化分析
腸道腺病毒%分子流行病學%基因進化分析
장도선병독%분자류행병학%기인진화분석
Enteric adenovirus%Molecular epidemiology%Phylogenetic analysis
目的 了解深圳地区感染性腹泻患者中肠道腺病毒的感染情况,并研究其分子流行病学特征.方法 采集深圳市2010年1月-2011年12月2083例疑似腹泻患者便标本,采用荧光PCR法检测肠道腺病毒核酸;应用特异性引物,对肠道腺病毒核酸阳性标本进一步扩增肠道腺病毒的hexon区,PCR扩增产物测序后,进行腺病毒型别鉴定和进化树分析,同时对病例的流行病学资料进行统计分析.结果 肠道腺病毒检出率为1.82％(38/2083);其中6月龄以下、6-11月龄、1-4岁和5岁以上患者检出分别为3.55％(6/169)、0.85％(6/707)、15.70％(19/121)和0.64％(7/1086);肠道腺病毒的感染无明显的季节变化特征,但不同月份检出率差异较大,介于0％ ～4.76％之间.对hexon区序列分析结果显示,深圳地区肠道腺病毒以41型为主要流行株,无40型肠道腺病毒的检出.结论 肠道腺病毒是深圳地区病毒性腹泻的重要病原体之一,41型肠道腺病毒是深圳地区肠道腺病毒的主要流行株,应加强对肠道腺病毒腹泻的监测.
目的 瞭解深圳地區感染性腹瀉患者中腸道腺病毒的感染情況,併研究其分子流行病學特徵.方法 採集深圳市2010年1月-2011年12月2083例疑似腹瀉患者便標本,採用熒光PCR法檢測腸道腺病毒覈痠;應用特異性引物,對腸道腺病毒覈痠暘性標本進一步擴增腸道腺病毒的hexon區,PCR擴增產物測序後,進行腺病毒型彆鑒定和進化樹分析,同時對病例的流行病學資料進行統計分析.結果 腸道腺病毒檢齣率為1.82％(38/2083);其中6月齡以下、6-11月齡、1-4歲和5歲以上患者檢齣分彆為3.55％(6/169)、0.85％(6/707)、15.70％(19/121)和0.64％(7/1086);腸道腺病毒的感染無明顯的季節變化特徵,但不同月份檢齣率差異較大,介于0％ ～4.76％之間.對hexon區序列分析結果顯示,深圳地區腸道腺病毒以41型為主要流行株,無40型腸道腺病毒的檢齣.結論 腸道腺病毒是深圳地區病毒性腹瀉的重要病原體之一,41型腸道腺病毒是深圳地區腸道腺病毒的主要流行株,應加彊對腸道腺病毒腹瀉的鑑測.
목적 료해심수지구감염성복사환자중장도선병독적감염정황,병연구기분자류행병학특정.방법 채집심수시2010년1월-2011년12월2083례의사복사환자편표본,채용형광PCR법검측장도선병독핵산;응용특이성인물,대장도선병독핵산양성표본진일보확증장도선병독적hexon구,PCR확증산물측서후,진행선병독형별감정화진화수분석,동시대병례적류행병학자료진행통계분석.결과 장도선병독검출솔위1.82％(38/2083);기중6월령이하、6-11월령、1-4세화5세이상환자검출분별위3.55％(6/169)、0.85％(6/707)、15.70％(19/121)화0.64％(7/1086);장도선병독적감염무명현적계절변화특정,단불동월빈검출솔차이교대,개우0％ ～4.76％지간.대hexon구서렬분석결과현시,심수지구장도선병독이41형위주요류행주,무40형장도선병독적검출.결론 장도선병독시심수지구병독성복사적중요병원체지일,41형장도선병독시심수지구장도선병독적주요류행주,응가강대장도선병독복사적감측.
Objective To investigate enteric adenovirus infection status and identify its molecular characteristics in Shenzhen.Methods From January 2010 to December 2011,2083 stool samples were collected from patients with suspected diarrhea and 30 stool samples were collected from healthy people in Shenzhen.The nucleic acids extracted from the samples were detected by real-time polymerase chain reaction (real-time PCR).The sequences of hexon of the enteric adenovirus positive samples were amplified by PCR,and then the products were sequenced after purification.The phylogenetic tree was constructed to analyze the nucleotide sequence homology between those sequences and the sequences of reference strains available in the GenBank database.Results 38 samples of 2083 were enteric adenovirus positive (1.82％,38/2083).The positive rates of patients under 6 months,6-11 months,1-4 years and above 5 years were 3.55％ (6/169),0.85％ (6/707),15.70％ (19/121) and 0.64％ (7/1086),respectively.The detection rates of EAdV in different months were between 0％ and 4.76％,with great fluctuation.There was no significant seasonal features of EAdV infection.According to the analysis on the gene sequencing,type 41 was the predominant strain of enteric adenovirus in Shenzhen,while no type 40 was detected.Conclusions Enteric adenovirus may play an important role in viral diarrhea in Shenzhen and type 41 was the most predominant strain.Therefore,it is necessary to strengthen the surveillance for diarrhea caused by enteric adenovirus infection.
