CAJ | 학술논문

目的探讨人疱疹病毒6型(human herpesvirus-6,HHV-6)感染与神经胶质瘤的关系.方法将辽宁省肿瘤医院神经外科2011年6月-2013年5月收治的神经胶质瘤患者45例纳入病例组,将同期该医院收治且已排除神经胶质瘤的脑外伤患者45例作为对照组.分别采用巢式聚合酶链式反应(nested polymerase chain reaction,Nested-PCR)法检测两组研究对象人病变脑组织样本中HHV-6序列;采用免疫组化染色法检测两组研究对象人病变脑组织样本中HHV-6抗原的表达.结果病例组HHV-6 DNA阳性率为31.11％,对照组HHV-6 DNA阳性率为6.67％ (x2=8.755,P=0.003).病例组HHV-6早期抗原p41表达阳性率为22.22％,对照组HHV-6早期抗原p41表达阳性率为0.00％(x2=11.250,P=0.001).病例组HHV-6 DNA阳性率、HHV-6抗原阳性率均高于对照组,组间差异有统计学意义(P＜0.05).结论神经胶质瘤患者和非神经胶质瘤脑外伤患者病变脑组织中HHV-6感染率有差异,据此推断HHV-6感染在神经胶质瘤的发生和发展过程中起到一定的作用.
목적 탐토인포진병독6형(human herpesvirus-6,HHV-6)감염여신경효질류적관계.방법 장요녕성종류의원신경외과2011년6월-2013년5월수치적신경효질류환자45례납입병례조,장동기해의원수치차이배제신경효질류적뇌외상환자45례작위대조조.분별채용소식취합매련식반응(nested polymerase chain reaction,Nested-PCR)법검측량조연구대상인병변뇌조직양본중HHV-6서렬;채용면역조화염색법검측량조연구대상인병변뇌조직양본중HHV-6항원적표체.결과 병례조HHV-6 DNA양성솔위31.11％,대조조HHV-6 DNA양성솔위6.67％ (x2=8.755,P=0.003).병례조HHV-6조기항원p41표체양성솔위22.22％,대조조HHV-6조기항원p41표체양성솔위0.00％(x2=11.250,P=0.001).병례조HHV-6 DNA양성솔、HHV-6항원양성솔균고우대조조,조간차이유통계학의의(P＜0.05).결론 신경효질류환자화비신경효질류뇌외상환자병변뇌조직중HHV-6감염솔유차이,거차추단HHV-6감염재신경효질류적발생화발전과정중기도일정적작용.
Objective To study the relationship of human herpes virus-6 (HHV-6) infection and glioma.Methods A total of 45 neurosurgery glioma patients who admitted to Shenyang tumour hospital from June 2011 to May 2013 were included in the case group,while another 45 cases with traumatic brain injury glioma were enrolled in the control group.Nested-PCR was used to detect HHV-6 DNA,immunohistochemistry method was used to detect the expression of HHV-6 in two groups.Results The HHV-6 DNA positive rate was 31.11％ (14/45) and 6.67％ (3/45) in case group and control group,respectively.There was statistical difference between them(x2 =8.755,P =0.003).The positive rate of p41 was 22.22％ (10/45)in case group,while no p41 antigen was checkout from control group,there was statistical difference between them (x2 =11.250,P =0.001).Conclusions HHV-6 infection play a part in the occurrence and development of glioma.