CAJ | 학술논문

目的监测北京市丰台区环境污水中的肠道病毒的血清型分布及流行情况,为北京市丰台区肠道病毒相关的传染病预防和控制提供实验室支持.方法选择吴家村污水处理厂为监测点,2012年每月采集污水(共12份),用阴离子膜-碱洗脱法进行病毒浓缩,然后进行病毒分离,并对阳性分离物进行肠道病毒血清型鉴定.结果北京市丰台区环境监测污水标本中共分离到60株肠道病毒、6株腺病毒;病毒分离以B组肠道病毒为主;夏季(5-9月)的病毒分离率较高;部分环境中分离的肠道病毒与手足口患者体内标本有同源性.结论环境监测可以为北京市丰台区肠道病毒病例监测系统提供重要的补充信息.本研究可以为丰台区乃至北京市的EV研究提供背景信息.
목적 감측북경시봉태구배경오수중적장도병독적혈청형분포급류행정황,위북경시봉태구장도병독상관적전염병예방화공제제공실험실지지.방법 선택오가촌오수처리엄위감측점,2012년매월채집오수(공12빈),용음리자막-감세탈법진행병독농축,연후진행병독분리,병대양성분리물진행장도병독혈청형감정.결과 북경시봉태구배경감측오수표본중공분리도60주장도병독、6주선병독;병독분리이B조장도병독위주;하계(5-9월)적병독분리솔교고;부분배경중분리적장도병독여수족구환자체내표본유동원성.결론 배경감측가이위북경시봉태구장도병독병례감측계통제공중요적보충신식.본연구가이위봉태구내지북경시적EV연구제공배경신식.
Objective To provide laboratory data for the prevention and control of the human enterovirus infection diseases in Fengtai district of Beijing,human enterovirus in enviromental sewage was monitored.Methods The Wu-jia-cun sewage treatment plant was chose as surveillance site,the sewage samples were collected monthly from in Fengtai district,from January 2012 to December 2012.Viral isolation was performed for the sewage samples.Results 60 enteroviruses and 6 adenoviruses were isolated from sewage water samples,HEV-B were the mainly enteroviruses during the environmental surveillance.Virus separation rate is higher in May,Jun,Jul,Aug and Sep.Part of the enterovirus isolated from environment specimens have homology with hand-foot-mouth of patients specimens.Conclusions Environmental surveillance can provide important supplementary information for enteroviruses case surveillance system which could provides background information on the EV study for the Fengtai district.