国际放射医学核医学杂志
國際放射醫學覈醫學雜誌
국제방사의학핵의학잡지
INTERNATIONAL JOURNAL OF RADIATION MEDICINE AND NUCLEAR MEDICINE
2013年
1期
5-8
,共4页
韩英%杨冰%唐泉%周慧%樊体强%樊飞跃%张晓东%孙元明
韓英%楊冰%唐泉%週慧%樊體彊%樊飛躍%張曉東%孫元明
한영%양빙%당천%주혜%번체강%번비약%장효동%손원명
辐射,电离%成骨细胞%破骨细胞%放射疗法%巨噬细胞集落刺激因子
輻射,電離%成骨細胞%破骨細胞%放射療法%巨噬細胞集落刺激因子
복사,전리%성골세포%파골세포%방사요법%거서세포집락자격인자
Radiation,ionizing%Osteoblasts%Osteoclasts%Radiotherapy%Macrophage colony-stimulating factor
目的 研究辐射对于原代成骨细胞巨噬细胞集落刺激因子(M-CSF)表达的影响,进而研究辐射导致骨损伤的分子机理.方法 采用原代骨髓间充质干细胞诱导分化为成骨细胞,经0、2、4 Gy 137Cs γ射线照射后,采用实时定量PCR以及Western blot方法检测辐射对M-CSF mRNA和蛋白表达水平的影响.结果 2Gy和4Gyγ射线照射均能引起成骨细胞M-CSF mRNA(t=-17.329,P＜0.01；t=-3.841,P＜0.05)和蛋白表达水平上调.4 Gy照射后则会导致成骨前体细胞M-CSF mRNA表达水平上调(t=-4.478,P＜0.05),但与对照组比较,两者的蛋白表达水平未见显著差异.结论 2 Gy和4Gyγ射线照射后,成骨细胞M-CSF表达水平上调能增强核因子κB受体活化因子配体对破骨细胞分化、成熟的促进作用,有助于增强破骨细胞的骨吸收能力.
目的 研究輻射對于原代成骨細胞巨噬細胞集落刺激因子(M-CSF)錶達的影響,進而研究輻射導緻骨損傷的分子機理.方法 採用原代骨髓間充質榦細胞誘導分化為成骨細胞,經0、2、4 Gy 137Cs γ射線照射後,採用實時定量PCR以及Western blot方法檢測輻射對M-CSF mRNA和蛋白錶達水平的影響.結果 2Gy和4Gyγ射線照射均能引起成骨細胞M-CSF mRNA(t=-17.329,P＜0.01；t=-3.841,P＜0.05)和蛋白錶達水平上調.4 Gy照射後則會導緻成骨前體細胞M-CSF mRNA錶達水平上調(t=-4.478,P＜0.05),但與對照組比較,兩者的蛋白錶達水平未見顯著差異.結論 2 Gy和4Gyγ射線照射後,成骨細胞M-CSF錶達水平上調能增彊覈因子κB受體活化因子配體對破骨細胞分化、成熟的促進作用,有助于增彊破骨細胞的骨吸收能力.
목적 연구복사대우원대성골세포거서세포집락자격인자(M-CSF)표체적영향,진이연구복사도치골손상적분자궤리.방법 채용원대골수간충질간세포유도분화위성골세포,경0、2、4 Gy 137Cs γ사선조사후,채용실시정량PCR이급Western blot방법검측복사대M-CSF mRNA화단백표체수평적영향.결과 2Gy화4Gyγ사선조사균능인기성골세포M-CSF mRNA(t=-17.329,P＜0.01；t=-3.841,P＜0.05)화단백표체수평상조.4 Gy조사후칙회도치성골전체세포M-CSF mRNA표체수평상조(t=-4.478,P＜0.05),단여대조조비교,량자적단백표체수평미견현저차이.결론 2 Gy화4Gyγ사선조사후,성골세포M-CSF표체수평상조능증강핵인자κB수체활화인자배체대파골세포분화、성숙적촉진작용,유조우증강파골세포적골흡수능력.
Objective To investigate the radiation effect on the expression of macrophage colony stimulating factor(M-CSF)in primary osteoblasts and the molecular mechanism of bone injury induced by radiation.Methods Osteoblasts differentiated from bone marrow stromal cells (BMSCs)were analyzed by real-time PCR and Western blot after 2 Gy or 4 Gy 137Cs γ-irradiation.Results M-CSF mRNA and protein expression level were up-regulated after 2 Gy and 4 Gy irradiation (t=-17.329,P＜0.01; t=-3.841,P＜0.05)in osteoblasts.4 Gy irradiation increased M-CSF mRNA in osteoblast precursors (t=-4.478,P＜0.05),but do not affect the protein expression level.Conclusions These results indicated that up-regulated M-CSF in osteoblasts could enhance the function of osteoclasts' differentiation and maturation which induced by receptor activator of nuclear factor κB ligand after 2 Gy and 4 Gy irradiation.Further more,up-regulated M-CSF expression could enhance osteoclastic bone resorption of mature osteoclasts.
