CAJ | 학술논문

目的探讨Tat-SmacN7蛋白(其中,Tat:反式激活蛋白；Smac:第二线粒体来源的caspase激活因子；N7:Smac的N端含有Ala-Val-Pro-Ile的七肽)对食管癌109(EC109)细胞放疗敏感性的影响.方法对EC109细胞给予Tat-SamcN7、照射、以及联合处理,观察不同处理在24 h和48 h时对细胞的抑制作用,采用Western blot检测各组细胞中Smac蛋白表达水平.结果通过水溶性四唑盐1检测发现Tat-SmacN7单独使用对细胞的抑制作用不明显,但与Tat-SmacN7组和照射组相比,Tat-SmacN7联合放射组可以明显增强对细胞的抑制(24h:t=16.821和9.825,P＜0.05；48 h:t=23.553和11.930,P＜0.05).说明Tat-SmacN7能增强EC109细胞对放射的敏感性.Western blot证明了Tat-SmacN7可以使细胞内Smac蛋白表达增加.结论 Tat-SmacN7蛋白能通过细胞高表达Smac,增强EC109细胞对辐射的敏感性.
목적 탐토Tat-SmacN7단백(기중,Tat:반식격활단백；Smac:제이선립체래원적caspase격활인자；N7:Smac적N단함유Ala-Val-Pro-Ile적칠태)대식관암109(EC109)세포방료민감성적영향.방법 대EC109세포급여Tat-SamcN7、조사、이급연합처리,관찰불동처리재24 h화48 h시대세포적억제작용,채용Western blot검측각조세포중Smac단백표체수평.결과 통과수용성사서염1검측발현Tat-SmacN7단독사용대세포적억제작용불명현,단여Tat-SmacN7조화조사조상비,Tat-SmacN7연합방사조가이명현증강대세포적억제(24h:t=16.821화9.825,P＜0.05；48 h:t=23.553화11.930,P＜0.05).설명Tat-SmacN7능증강EC109세포대방사적민감성.Western blot증명료Tat-SmacN7가이사세포내Smac단백표체증가.결론 Tat-SmacN7단백능통과세포고표체Smac,증강EC109세포대복사적민감성.
Objective To investigate the effects of Tat-SmacN7 protein on sensitivity of esophageal carcinoma 109 (EC109) to radiation.Methods Cells were treated with Tat-SmacN7 peptides,radiation or combination,the inhibition rate of EC109 cells were detected by water-soluble tetrazolium salt-1 assay.The protein level expression of Smac was determined by Western blot.Results The cells were resistant to TatSmacN7 as a single agent,but it could improve the sensitization of EC 109 to radiation.Compared with the TatSamcN7 group and the radiation group,the Tat-SmacN7 combined with radiation group was inhibited significantly (24 h:t=16.821 and 9.825,both P＜0.05; 48 h:t=23.553 and 11.930,both P＜0.05).Western blot assay showed that Tat-SmacN7 could increase the expression of Smac protein.Conclusions Tat-SmacN7 could improve the radiosensitization of EC 109 cell by increasing the expression of Smac.