CAJ | 학술논문

噬菌体展示技术是研究分子间多种作用的技术,它能够在机理尚未明确的情况下研究蛋白质与蛋白质、蛋白质与多肽、蛋白质与核酸的相互作用,这种技术的一个关键优势是,它可以研究分子间的直接联系,得到不同亲和力的分子,一次性实现高效筛选多肽的目的.近年来随着噬菌体展示技术的成熟,该技术被广泛应用于生命科学研究的不同领域,如:单克隆抗体制备、多肽筛选、疫苗研制、基因治疗及细胞信号转导研究等.该文综述了噬菌体展示技术在分子核医学相关研究中的运用.
서균체전시기술시연구분자간다충작용적기술,타능구재궤리상미명학적정황하연구단백질여단백질、단백질여다태、단백질여핵산적상호작용,저충기술적일개관건우세시,타가이연구분자간적직접련계,득도불동친화력적분자,일차성실현고효사선다태적목적.근년래수착서균체전시기술적성숙,해기술피엄범응용우생명과학연구적불동영역,여:단극륭항체제비、다태사선、역묘연제、기인치료급세포신호전도연구등.해문종술료서균체전시기술재분자핵의학상관연구중적운용.
Phage display is a molecular diversity technology that allows the presentation of large peptide and protein libraries on the surface of filamentous phage.Phage display libraries permit the selection of peptides and proteins,including antibodies,with high affinity and specificity for almost any target.In recent years,along with phage display technology maturated,it has been widely used in screening tumor antibody library,the preparation of monoclonal antibody,polypeptide,drug design,gene therapy and cell signal transduction.This review serves as an introduction to phage display in molecular nuclear medicine,and recent application in display technology.