CAJ | 학술논문

目的构建靶向FoxM1基因的短发夹RNA(shRNA)干扰表达载体质粒,为进一步探讨FoxM1在肺部恶性肿瘤及其他疾病中的作用奠定基础.方法化学合成可编码靶向FoxM1基因的siRNA的短发夹DNA (shDNA)片段,通过基因重组将其插入到线性化的shRNA表达质粒骨架pSilencer 2.1-U6中,对得到的新质粒进行酶切鉴定和基因测序.结果所构建的质粒载体中成功插入了可编码靶向FoxM1基因的siRNA的shDNA片段.结论成功构建了靶向FoxM1基因的shDNA表达载体.
목적 구건파향FoxM1기인적단발협RNA(shRNA)간우표체재체질립,위진일보탐토FoxM1재폐부악성종류급기타질병중적작용전정기출.방법 화학합성가편마파향FoxM1기인적siRNA적단발협DNA (shDNA)편단,통과기인중조장기삽입도선성화적shRNA표체질립골가pSilencer 2.1-U6중,대득도적신질립진행매절감정화기인측서.결과 소구건적질립재체중성공삽입료가편마파향FoxM1기인적siRNA적shDNA편단.결론 성공구건료파향FoxM1기인적shDNA표체재체.
Objective To construct a siRNA expressing vector targeting FoxM1,in order to investigate the effect of FoxM1 gene in lung cancer and other diseases.Methods A FoxM1 targeting short hairpin DNA fragment was designed and synthesized,and then was cloned into a linearized backbone plasmid pSilencer2.l-U6.The new plasmid was identified to be correct by cutting with restriction enzymes and gene sequencing.Results The FoxM1 targeting shDNA fragment was inserted into pSilencer 2.1-U6 backbone vector correctly.Conclusions The small interfering RNA expression vector targeting FoxM1 gene is constructed successfully.