CAJ | 학술논문

目的研究克林霉素对小鼠社区获得性耐甲氧西林金黄色葡萄球菌(CA-MRSA)肺炎的疗效.方法将63只小鼠随机分为对照组、高菌量组和低菌量组,分别经鼻滴入等体积的PBS、高浓度CA-MRSA菌液和低浓度CA-MRSA菌液.感染后6h再分别将高菌量组和低菌量组小鼠随机分为感染组和克林霉素组,分别经腹腔注射双蒸水和克林霉素溶液.观察高菌量组小鼠的生存时间.检测低菌量组小鼠的肺部载菌量和病理变化,支气管肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)和巨噬细胞炎症蛋白-2(MIP-2)的浓度、白细胞和中性粒细胞计数.结果感染组小鼠的生存率为33.3％,肺组织中有大量细菌繁殖,肺组织实变,甚至坏死,BALF中TNF-α和MIP-2的浓度、白细胞和中性粒细胞计数均较对照组明显升高.与感染组比较,克林霉素组小鼠的生存率提高,肺组织的载菌量、病理损伤、炎症指标均明显减轻.结论本实验成功建立了小鼠CA-MRSA肺炎模型,克林霉素对CA-MRSA肺炎有重要的治疗价值.
목적 연구극림매소대소서사구획득성내갑양서림금황색포도구균(CA-MRSA)폐염적료효.방법 장63지소서수궤분위대조조、고균량조화저균량조,분별경비적입등체적적PBS、고농도CA-MRSA균액화저농도CA-MRSA균액.감염후6h재분별장고균량조화저균량조소서수궤분위감염조화극림매소조,분별경복강주사쌍증수화극림매소용액.관찰고균량조소서적생존시간.검측저균량조소서적폐부재균량화병리변화,지기관폐포관세액(BALF)중종류배사인자α(TNF-α)화거서세포염증단백-2(MIP-2)적농도、백세포화중성립세포계수.결과 감염조소서적생존솔위33.3％,폐조직중유대량세균번식,폐조직실변,심지배사,BALF중TNF-α화MIP-2적농도、백세포화중성립세포계수균교대조조명현승고.여감염조비교,극림매소조소서적생존솔제고,폐조직적재균량、병리손상、염증지표균명현감경.결론 본실험성공건립료소서CA-MRSA폐염모형,극림매소대CA-MRSA폐염유중요적치료개치.
Objective To investigate the efficacy of clindamycin in the treatment of communityacquired methicillin-resistant Staphylococcus aureus (CA-MRSA) pneumonia in mice.Methods 63BALB/c mice were randomly divided into control group,high and low inoculation group,which were respectively administrated intranasally PBS,high and low CA-MRSA inoculum.At 6 h after inoculation,mice from high and low CA-MRSA inoculation group were,then respectively divided into infection group and clindamycin group,which were respectively administrated double distilled water and clindamycin by intraperitoneal injection.The survival time of the mice from high inoculation group was observed.For the mice from low inoculation group,the bacteria loading and histopathology changes in the lung were investigated.In bronchoalveolar lavage fluid (BALF),the levels of tumor necrosis factor-α and macrophage inflammatory protein-2 were measured and the leukocytes and neutrophil were counted.Results Compared with the mice from control group,those from infection group presented lower survivalrate,higher bacteria loading,and more severe damage in the lung,as well as more inflammatory cells and inflammatory factors in BALF.In addition,the survival rate,bacteria loading,pathological changes and inflammatory reactions were significantly improved in the mice from clindamycin group compared with those from infection group.Conclusions Our study indicates that clindamycin is an attractive option for the treatment of CA MRSA pneumonia.