国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2013年
10期
726-729,封3
,共5页
陈艳%陈一强%孔晋亮%巫艳彬%蔡双启%杜仲业%黄莹莹%蒙锦祥
陳豔%陳一彊%孔晉亮%巫豔彬%蔡雙啟%杜仲業%黃瑩瑩%矇錦祥
진염%진일강%공진량%무염빈%채쌍계%두중업%황형형%몽금상
耐甲氧西林金黄色葡萄球菌%生物被膜%黄芩素%万古霉素%克拉霉素
耐甲氧西林金黃色葡萄毬菌%生物被膜%黃芩素%萬古黴素%剋拉黴素
내갑양서림금황색포도구균%생물피막%황금소%만고매소%극랍매소
Methicillin resistant Staphylococcus aureus%Biofilm%Baicalein%Vancomycin%Clarithromycin
目的 通过建立体外耐甲氧西林金黄色葡萄球菌(MRSA)早期生物被膜(BF)模型,研究黄芩素对BF的影响及其联合万古霉素(VAN)的协同杀菌效果.方法 选取临床分离并且能够稳定形成BF的MRSA 17546,采用胰蛋白胨大豆肉汤添加0.5％葡萄糖为培养基,复制体外早期BF模型,实验分为空白对照组、阳性对照组[16 mg/L克拉霉素(CLR)组、4 mg/L VAN组、16 mg/L CLR+4 mg/LVAN组]、实验组(32 mg/L黄芩素组、32 mg/L黄芩素+4 mg/L VAN组).试管二倍稀释法测定药物的最低抑菌浓度,连续稀释法进行活菌计数,扫描电镜观察BF的形态结构.结果 早期BF经VAN、CLR及黄芩素作用后BF内活菌数分别为(7.95±0.19)、(8.03±0.23)、(7.95±0.18) log10 cfu/ml,与空白对照组[(7.99±0.25) log10 cfu/m1]相比差异无统计学意义(P＞0.05).16 mg/LCLR+4 mg/LVAN组、32mg/L黄芩素+4 mg/L VAN组活菌数为(7.71±0.25)、(7.29±0.16) log10cfu/ml,低于空白对照组(P＜0.01),且32 mg/L黄芩素+4 mg/L VAN组比16 mg/L CLR+4 mg/L VAN组活菌数进一步减少(P＜0.05).经扫描电镜观察,可见黄芩素组载体上的BF比空白对照组减少,VAN组BF内细菌数未见明显减少,而黄芩素+VAN组BF内的细菌数明显比空白对照组减少.结论 黄芩素能破坏MRSA已形成的早期BF,增强VAN对BF内MRSA的清除作用,且黄芩素破坏BF及其协同杀菌作用强于CLR.
目的 通過建立體外耐甲氧西林金黃色葡萄毬菌(MRSA)早期生物被膜(BF)模型,研究黃芩素對BF的影響及其聯閤萬古黴素(VAN)的協同殺菌效果.方法 選取臨床分離併且能夠穩定形成BF的MRSA 17546,採用胰蛋白胨大豆肉湯添加0.5％葡萄糖為培養基,複製體外早期BF模型,實驗分為空白對照組、暘性對照組[16 mg/L剋拉黴素(CLR)組、4 mg/L VAN組、16 mg/L CLR+4 mg/LVAN組]、實驗組(32 mg/L黃芩素組、32 mg/L黃芩素+4 mg/L VAN組).試管二倍稀釋法測定藥物的最低抑菌濃度,連續稀釋法進行活菌計數,掃描電鏡觀察BF的形態結構.結果 早期BF經VAN、CLR及黃芩素作用後BF內活菌數分彆為(7.95±0.19)、(8.03±0.23)、(7.95±0.18) log10 cfu/ml,與空白對照組[(7.99±0.25) log10 cfu/m1]相比差異無統計學意義(P＞0.05).16 mg/LCLR+4 mg/LVAN組、32mg/L黃芩素+4 mg/L VAN組活菌數為(7.71±0.25)、(7.29±0.16) log10cfu/ml,低于空白對照組(P＜0.01),且32 mg/L黃芩素+4 mg/L VAN組比16 mg/L CLR+4 mg/L VAN組活菌數進一步減少(P＜0.05).經掃描電鏡觀察,可見黃芩素組載體上的BF比空白對照組減少,VAN組BF內細菌數未見明顯減少,而黃芩素+VAN組BF內的細菌數明顯比空白對照組減少.結論 黃芩素能破壞MRSA已形成的早期BF,增彊VAN對BF內MRSA的清除作用,且黃芩素破壞BF及其協同殺菌作用彊于CLR.
목적 통과건입체외내갑양서림금황색포도구균(MRSA)조기생물피막(BF)모형,연구황금소대BF적영향급기연합만고매소(VAN)적협동살균효과.방법 선취림상분리병차능구은정형성BF적MRSA 17546,채용이단백동대두육탕첨가0.5％포도당위배양기,복제체외조기BF모형,실험분위공백대조조、양성대조조[16 mg/L극랍매소(CLR)조、4 mg/L VAN조、16 mg/L CLR+4 mg/LVAN조]、실험조(32 mg/L황금소조、32 mg/L황금소+4 mg/L VAN조).시관이배희석법측정약물적최저억균농도,련속희석법진행활균계수,소묘전경관찰BF적형태결구.결과 조기BF경VAN、CLR급황금소작용후BF내활균수분별위(7.95±0.19)、(8.03±0.23)、(7.95±0.18) log10 cfu/ml,여공백대조조[(7.99±0.25) log10 cfu/m1]상비차이무통계학의의(P＞0.05).16 mg/LCLR+4 mg/LVAN조、32mg/L황금소+4 mg/L VAN조활균수위(7.71±0.25)、(7.29±0.16) log10cfu/ml,저우공백대조조(P＜0.01),차32 mg/L황금소+4 mg/L VAN조비16 mg/L CLR+4 mg/L VAN조활균수진일보감소(P＜0.05).경소묘전경관찰,가견황금소조재체상적BF비공백대조조감소,VAN조BF내세균수미견명현감소,이황금소+VAN조BF내적세균수명현비공백대조조감소.결론 황금소능파배MRSA이형성적조기BF,증강VAN대BF내MRSA적청제작용,차황금소파배BF급기협동살균작용강우CLR.
Objective To investigate the effect of baicalein in combination with vancomycin (VAN)on methicillin-resistant Staphylococcus aureus (MRSA) early biofilm.Methods Clinical isolates of MRSA 17546 which could form biofilm stably,were cultured in TSB G medium for biofilm formation in three days.MIC was measured by doubling dilution.Viable bacterial counts were determined by serial dilution.Biofilm was observed by scanning electron microscope (SEM).Results After the early biofim was reacted with 4 mg/L VAN,16 mg/L clarithromycin (CLR) or 32 mg/L baicalein for 12 hours,the biofilm bacterial counts were (7.95 ± 0.19),(8.03 ± 0.23),(7.95 ± 0.18) log10 cfu/ml,there was no statistical significance compared to the control group [(7.99±0.25) log10 cfu/ml,P ＞0.05].The counts of the CLR plus VAN group and the baicalein plus VAN group were (7.71 ± 0.25),(7.29±0.16)log10 cfu/ml,which were significantly less than that of the control group (P ＜0.01),and the counts of the baicalein plus VAN group was less than that of the CLR plus VAN group (P ＜0.05).Besides,the biofilm of the baicalein group was less than the control group,the bacteria counts of the VAN group showed no decrease,and the baicalein plus vancomycin group showed obvious decrease compare to the control group observed by SEM.Conclusions Baicalein can destroy the early biofilm of MRSA in vitro and enhance susceptibility of VAN to MRSA within biofilm.
