CAJ | 학술논문

目的观察槐耳清膏对人肺腺癌细胞系A549细胞增殖和自噬的影响,探讨其作用机制.方法以A549细胞为研究对象,采用CCK-8法检测槐耳清膏对A549的增殖抑制率,用Western blot 方法检测自噬相关蛋白P62、LC3B表达的变化和mTOR信号通路分子表达的变化.结果 0.5 g/L及以上的槐耳清膏可明显抑制A549细胞的增殖,并且具有时间和浓度依赖性(P＜0.05).用不同浓度(1、2、4、6、8 g/L)的槐耳清膏作用A549细胞48 h可以降低P62表达水平,增加LC3B的表达,降低mTOR信号通路关键分子的表达水平.联合使用自噬抑制剂3-MA可以加强槐耳清膏对A549细胞的增殖抑制率.结论槐耳清膏通过下调mTOR信号通路抑制A549细胞增殖,并且诱导A549发生自噬.联合使用槐耳和自噬抑制剂可能会更有效发挥槐耳抗肿瘤增殖的作用.
목적 관찰괴이청고대인폐선암세포계A549세포증식화자서적영향,탐토기작용궤제.방법 이A549세포위연구대상,채용CCK-8법검측괴이청고대A549적증식억제솔,용Western blot 방법검측자서상관단백P62、LC3B표체적변화화mTOR신호통로분자표체적변화.결과 0.5 g/L급이상적괴이청고가명현억제A549세포적증식,병차구유시간화농도의뢰성(P＜0.05).용불동농도(1、2、4、6、8 g/L)적괴이청고작용A549세포48 h가이강저P62표체수평,증가LC3B적표체,강저mTOR신호통로관건분자적표체수평.연합사용자서억제제3-MA가이가강괴이청고대A549세포적증식억제솔.결론 괴이청고통과하조mTOR신호통로억제A549세포증식,병차유도A549발생자서.연합사용괴이화자서억제제가능회경유효발휘괴이항종류증식적작용.
Objective To investigate the effects of Huaier on cell growth and autophagy in A549 cell line.Methods Cell growth was measured by CCK-8 assay kit,Western blot was used to test the autophagy effect and mTOR pathway.Results Huaier inhibited the proliferation of A549 in a time-anddose-dependent manner (P ＜ 0.05).Western blot analysis revealed cell autophagy by LC3B Ⅱ/Ⅰconversion and decreased P62.Western blot also indicated the downregulated mTOR signaling pathway induced by the increased concentration of Huaier.Combination treatment of autophagy inhibitor 3-MA and Huaier reinforced the inhibition effects.Conclusions Huaier inhibits the growth of A549 via downregulating mTOR pathway and induces autophagy.Combination therapy of Huaier and autophagy inhibitors are potentially more effective in inhibition of tumor cells than Huaier only.