目的 研究全反式维甲酸(ATRA)对木瓜蛋白酶所致大鼠肺气肿模型的早期干预作用.方法 采用木瓜蛋白酶气管内滴注的方法,将体质量170～220 g 60只SPF级SD大鼠随机分为对照组(N),模型组(R),ATRA治疗组(R1),棉籽油组(R2)各15只.后3组向大鼠气管内滴注5％木瓜蛋白酶1 ml/kg,N组同样滴注等量生理盐水,14d后,分别向R1组和R2组大鼠腹腔内连续注入1 000 μg/kgATRA和棉籽油,第90天杀死全部大鼠.取出肺组织,行HE染色观察各组大鼠的肺脏的病理变化,和病理形态学图像分析了解肺气肿模型是否建立及ATRA对于木瓜蛋白酶复制的大鼠肺气肿模型的早期干预效果.行免疫组织化学染色,计算各组Ⅱ型肺泡上皮细胞(AECⅡ)凋亡率,及Bax蛋白、Bcl-2蛋白阳性细胞个数,比较各组之间AECⅡ凋亡率,及Bax蛋白、Bcl-2蛋白阳性细胞个数的差异.结果 肺体积结果显示:R、R1、R2组的肺体积与N组比较,明显增大；R、R2组之间差异无统计学意义.肺泡形态学结果显示:R、R1、R2与N组比较,每个视野内的肺泡数明显减少,平均肺泡面积增大,平均内衬间隔较大,R2组相对于R组,肺泡形态学各项指标差异无统计学意义.R、R1、R2组与N组比较,大鼠肺组织AECⅡ凋亡明显增多,R1与R组比较,AECⅡ凋亡率明显下降,R2与R组比较,AECⅡ凋亡率无明显变化,R、R1、R2组在造模后肺泡壁细胞Bcl-2阳性细胞百分比显著高于N组,R组肺泡壁细胞Bcl-2阳性细胞百分比显著低于R1组,R组肺泡壁细胞Bcl-2阳性细胞百分比与R2组差异无统计学意义.R、R1、R2组在造模后肺泡壁Bax蛋白阳性细胞百分比显著高于N组,R组肺泡壁细胞Bax蛋白阳性细胞百分比显著高于R1组,R组肺泡壁Bax蛋白阳性细胞百分比与R2组差异无统计学意义.R1组Bcl-2/Bax比例明显高于R、R2组,而与N组差异无统计学意义.结论 ATRA治疗可降低大鼠肺气肿模型AECⅡ凋亡率,调节Bax蛋白、Bcl-2蛋白表达,对大鼠肺气肿进程起到干预作用.
目的 研究全反式維甲痠(ATRA)對木瓜蛋白酶所緻大鼠肺氣腫模型的早期榦預作用.方法 採用木瓜蛋白酶氣管內滴註的方法,將體質量170～220 g 60隻SPF級SD大鼠隨機分為對照組(N),模型組(R),ATRA治療組(R1),棉籽油組(R2)各15隻.後3組嚮大鼠氣管內滴註5％木瓜蛋白酶1 ml/kg,N組同樣滴註等量生理鹽水,14d後,分彆嚮R1組和R2組大鼠腹腔內連續註入1 000 μg/kgATRA和棉籽油,第90天殺死全部大鼠.取齣肺組織,行HE染色觀察各組大鼠的肺髒的病理變化,和病理形態學圖像分析瞭解肺氣腫模型是否建立及ATRA對于木瓜蛋白酶複製的大鼠肺氣腫模型的早期榦預效果.行免疫組織化學染色,計算各組Ⅱ型肺泡上皮細胞(AECⅡ)凋亡率,及Bax蛋白、Bcl-2蛋白暘性細胞箇數,比較各組之間AECⅡ凋亡率,及Bax蛋白、Bcl-2蛋白暘性細胞箇數的差異.結果 肺體積結果顯示:R、R1、R2組的肺體積與N組比較,明顯增大；R、R2組之間差異無統計學意義.肺泡形態學結果顯示:R、R1、R2與N組比較,每箇視野內的肺泡數明顯減少,平均肺泡麵積增大,平均內襯間隔較大,R2組相對于R組,肺泡形態學各項指標差異無統計學意義.R、R1、R2組與N組比較,大鼠肺組織AECⅡ凋亡明顯增多,R1與R組比較,AECⅡ凋亡率明顯下降,R2與R組比較,AECⅡ凋亡率無明顯變化,R、R1、R2組在造模後肺泡壁細胞Bcl-2暘性細胞百分比顯著高于N組,R組肺泡壁細胞Bcl-2暘性細胞百分比顯著低于R1組,R組肺泡壁細胞Bcl-2暘性細胞百分比與R2組差異無統計學意義.R、R1、R2組在造模後肺泡壁Bax蛋白暘性細胞百分比顯著高于N組,R組肺泡壁細胞Bax蛋白暘性細胞百分比顯著高于R1組,R組肺泡壁Bax蛋白暘性細胞百分比與R2組差異無統計學意義.R1組Bcl-2/Bax比例明顯高于R、R2組,而與N組差異無統計學意義.結論 ATRA治療可降低大鼠肺氣腫模型AECⅡ凋亡率,調節Bax蛋白、Bcl-2蛋白錶達,對大鼠肺氣腫進程起到榦預作用.
목적 연구전반식유갑산(ATRA)대목과단백매소치대서폐기종모형적조기간예작용.방법 채용목과단백매기관내적주적방법,장체질량170～220 g 60지SPF급SD대서수궤분위대조조(N),모형조(R),ATRA치료조(R1),면자유조(R2)각15지.후3조향대서기관내적주5％목과단백매1 ml/kg,N조동양적주등량생리염수,14d후,분별향R1조화R2조대서복강내련속주입1 000 μg/kgATRA화면자유,제90천살사전부대서.취출폐조직,행HE염색관찰각조대서적폐장적병리변화,화병리형태학도상분석료해폐기종모형시부건립급ATRA대우목과단백매복제적대서폐기종모형적조기간예효과.행면역조직화학염색,계산각조Ⅱ형폐포상피세포(AECⅡ)조망솔,급Bax단백、Bcl-2단백양성세포개수,비교각조지간AECⅡ조망솔,급Bax단백、Bcl-2단백양성세포개수적차이.결과 폐체적결과현시:R、R1、R2조적폐체적여N조비교,명현증대；R、R2조지간차이무통계학의의.폐포형태학결과현시:R、R1、R2여N조비교,매개시야내적폐포수명현감소,평균폐포면적증대,평균내츤간격교대,R2조상대우R조,폐포형태학각항지표차이무통계학의의.R、R1、R2조여N조비교,대서폐조직AECⅡ조망명현증다,R1여R조비교,AECⅡ조망솔명현하강,R2여R조비교,AECⅡ조망솔무명현변화,R、R1、R2조재조모후폐포벽세포Bcl-2양성세포백분비현저고우N조,R조폐포벽세포Bcl-2양성세포백분비현저저우R1조,R조폐포벽세포Bcl-2양성세포백분비여R2조차이무통계학의의.R、R1、R2조재조모후폐포벽Bax단백양성세포백분비현저고우N조,R조폐포벽세포Bax단백양성세포백분비현저고우R1조,R조폐포벽Bax단백양성세포백분비여R2조차이무통계학의의.R1조Bcl-2/Bax비례명현고우R、R2조,이여N조차이무통계학의의.결론 ATRA치료가강저대서폐기종모형AECⅡ조망솔,조절Bax단백、Bcl-2단백표체,대대서폐기종진정기도간예작용.
Objective To study the all-trans-retinoic acid (ATRA) to be caused by papain rat emphysema model of early intervention role.Methods The papain endotracheal drip method,weight 170-220 grams only 60 SPF SD rats randomly divided into the control group (N),model group (R),ATRA treatment group (R1),cottonseed oil group (R2) the only.After the three groups to rat endotracheal drip 5 ％ papain,1 ml/kg,N group also releases physiological saline drip,14 days,separately to the treatment group and rats,cottonseed oil intra-abdominal continuous inject 1 000 μg/kg ATRA and cottonseed oil,day 90 kill all rats.Take out the lung tissue,did HE dyeing observation group of rats lung pathological changes,and pathological morphology image analysis model is established and understand emphysema ATRA for papain copy of the rats emphysema model of early intervention effect.Do immunohistochemical stains,the calculation of the type Ⅱ aleolar epithelial cells (AEC Ⅱ) apoptosis rate,and Bax protein,Bcl-2 protein positive cell number,comparison between groups Ⅱ AEC apoptosis ate,and Bax protein,Bcl-2 protein positive cells of the number of differences.Results The results show that the lung volume:R,R1,R2 group of lung volume and N group than in the increased obviously.R,R2 no difference between groups.The alveolar morphological results show:R,R1,R2 group compared with N,Na significantly reduced,MAA increases,MLI bigger,R2 group compared with R group,the alveolar morphological index no obvious difference.R,R1,R2 group compared with N,rats AEC lung tissue Ⅱ apoptosis significantly increased,R1 group compared with R,AEC Ⅱ apoptosis rate decreased obviously,R2 group compared with R,AEC Ⅱ apoptosis rate no change,R,R1,R2 group made in the alveolar walls cells Bcl-2 percentage positive cells significantly higher than N group,R group of alveolar walls cells Bcl-2 positive cells percentage significantly lower than R1 group,R group of alveolar walls cells Bcl-2 percentage positive cells with R2 group there were no obvious difference.R,R1,R2 group made in the alveolar walls Bax protein positive cells percentage significantly higher than normal control group,R group of alveolar walls cells Bax protein positive cells percentage significantly higher than R1 group,R group of alveolar walls Bax protein positive cells percentage and R2 group there were no obvious difference.R1 group Bcl-2/Bax scale was significantly higher than the R,R2 group,and had no obvious difference with N group.Conclusions ATRA treatment can reduce rat emphysema model Ⅱ AEC apoptosis rate,adjust Bax protein and Bcl-2 protein expression,to rat emphysema process have intervention role,cottonseed oil as solvent to rat emphysema process without intervention role.
