国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2013年
19期
1464-1466
,共3页
大鼠%水通道蛋白-1%低氧性肺动脉高压
大鼠%水通道蛋白-1%低氧性肺動脈高壓
대서%수통도단백-1%저양성폐동맥고압
Rats%Aquaporin-1%Hypoxic pulmonary hypertension
目的 研究大鼠在高原低氧环境下肺动脉高压形成过程中水通道蛋白-1(aquaporin-1,AQP-1)表达的变化和意义.方法 将60只200～280 g的雄性Wistar大鼠分为3组,对照组1组和实验组3组；对照组为平原对照组；实验组分别为高原低氧环境10 d、20 d、30 d组.高原低氧组大鼠置于模拟海拔5 000 m低压低氧舱,每天22 h,对照组大鼠在平原环境饲养；应用西门子6002有创血压监测系统,测定平均肺动脉压(MPAP),而后取出肺组织标本做免疫组织化学染色.结果 ①MPAP:高原低氧10d、20 d、30 d组与对照组比较P值均＜0.05；②AQP-1:高原低氧10 d、20 d、30 d组与对照组比较,P ＜0.05；③相关性分析:大鼠MPAP、AQP-1呈正相关.结论 AQP-1在高原低氧环境下表达增高,与肺动脉压的严重程度相关.
目的 研究大鼠在高原低氧環境下肺動脈高壓形成過程中水通道蛋白-1(aquaporin-1,AQP-1)錶達的變化和意義.方法 將60隻200～280 g的雄性Wistar大鼠分為3組,對照組1組和實驗組3組；對照組為平原對照組；實驗組分彆為高原低氧環境10 d、20 d、30 d組.高原低氧組大鼠置于模擬海拔5 000 m低壓低氧艙,每天22 h,對照組大鼠在平原環境飼養；應用西門子6002有創血壓鑑測繫統,測定平均肺動脈壓(MPAP),而後取齣肺組織標本做免疫組織化學染色.結果 ①MPAP:高原低氧10d、20 d、30 d組與對照組比較P值均＜0.05；②AQP-1:高原低氧10 d、20 d、30 d組與對照組比較,P ＜0.05；③相關性分析:大鼠MPAP、AQP-1呈正相關.結論 AQP-1在高原低氧環境下錶達增高,與肺動脈壓的嚴重程度相關.
목적 연구대서재고원저양배경하폐동맥고압형성과정중수통도단백-1(aquaporin-1,AQP-1)표체적변화화의의.방법 장60지200～280 g적웅성Wistar대서분위3조,대조조1조화실험조3조；대조조위평원대조조；실험조분별위고원저양배경10 d、20 d、30 d조.고원저양조대서치우모의해발5 000 m저압저양창,매천22 h,대조조대서재평원배경사양；응용서문자6002유창혈압감측계통,측정평균폐동맥압(MPAP),이후취출폐조직표본주면역조직화학염색.결과 ①MPAP:고원저양10d、20 d、30 d조여대조조비교P치균＜0.05；②AQP-1:고원저양10 d、20 d、30 d조여대조조비교,P ＜0.05；③상관성분석:대서MPAP、AQP-1정정상관.결론 AQP-1재고원저양배경하표체증고,여폐동맥압적엄중정도상관.
Objective To study the expression and singnificance of AQP-1 in lung tissue of rats with hypoxic pulmonary hypertension.Methods 60 Wistar male rats which weight rang from 200 g to 280 g were divided into four groups:control group,altitude hypoxia environment 10 d,20 d,30 d group,measured the pulmonary artery pressure of each group and AQP-1 immunohistochemical staining in lung tissue,analysis the expression AQP-1 and the relationship with pulmonary artery pressur.Results The expression intensity of AQP-I and pulmonary artery pressure gradually increased with altitude hypoxia time extension,the expression of AQP-1 and the pulmonary artery pressure slowly increase with time extent.Conclusions AQP-1 and pulmonary artery pressure was positively correlated at the process of altitude hypoxia environment pulmonary hypertension,AQP-1 may play an important role in the development of pulmonary hypertension of hypoxia environment,by regulating the expression of AQP-1 may prevent pulmonary hypertension.
