国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2013年
20期
1529-1533
,共5页
卢宏志%高国芹%李昨飞%单冰竹
盧宏誌%高國芹%李昨飛%單冰竹
로굉지%고국근%리작비%단빙죽
杂种犬%急性肺动脉高压%炎症反应%单核细胞趋化蛋白-1
雜種犬%急性肺動脈高壓%炎癥反應%單覈細胞趨化蛋白-1
잡충견%급성폐동맥고압%염증반응%단핵세포추화단백-1
Mongrel dogs%Acute pulmonary hypertension%Inflammatory response%Monocyte chemoattractant protein-1
目的 探讨硫酸镁(MgSO4)对急性肺栓塞肺动脉高压的影响及其抗炎机制.方法 30只雄性杂种犬,体质量(15±2.1) kg,随机分为5组:空白组、对照组以及MgSO47.0组、MgSO48.0组、MgSO4 9.0组,每组6只,其中空白组不给予任何处理；对照组造成急性肺动脉高压；其余三组造成急性肺动脉高压后给予MgSO4静滴,稳定30 min后,MgSO4 200 mg/kg静脉滴注0.5h,然后维持血MgSO4浓度分别为7.0 mmol/L、8.0 mmol/L以及9.0 mmol/L.肺动脉、股动脉插管检测平均肺动脉压(MPAP)、体循环平均动脉压(MAP),应用酶联免疫吸附以及放免法分别测定血清中白介素6(IL-6)和肿瘤坏死因子α(TNF-α),Western blot测定肺组织中单核细胞趋化蛋白-1(MCP-1)蛋白表达.结果 ①对照组MPAP较空白组显著升高(P＜0.01),MgSO4 7.0组、MgSO48.0组以及MgSO49.0组MPAP较对照组显著下降(P＜0.05或P＜0.01),其中MgSO49.0组下降最明显；对照组MAP较空白组显著降低(P ＜0.05或P＜0.01),MgSO4 7.0组、MgSO48.0组以及MgSO49.0组MAP较对照组显著升高(P ＜0.05或P＜0.01),其中MgSO49.0组升高最明显.②对照组血清中TNF-α以及IL-6较空白组升高(P＜0.05),MgSO4 7.0组、MgSO4 8.0组以及MgSO4 9.0组TNF-α以及IL-6较对照组显著下降(P＜0.05或P＜0.01),MgSO4 9.0组下降最明显；③对照组MCP-1蛋白表达较空白组显著升高(P＜0.01),MgSO4 7.0组、MgSO4 8.0组以及MgSO49.0组MCP-1蛋白表达较对照组显著下降(P＜0.01),MgSO49.0组蛋白表达最低.结论 MgSO4可显著降低肺动脉高压,这可能与其抑制肺组织MCP-1表达,降低TNF-α以及IL-6,从而抑制炎症反应增强有关.
目的 探討硫痠鎂(MgSO4)對急性肺栓塞肺動脈高壓的影響及其抗炎機製.方法 30隻雄性雜種犬,體質量(15±2.1) kg,隨機分為5組:空白組、對照組以及MgSO47.0組、MgSO48.0組、MgSO4 9.0組,每組6隻,其中空白組不給予任何處理；對照組造成急性肺動脈高壓；其餘三組造成急性肺動脈高壓後給予MgSO4靜滴,穩定30 min後,MgSO4 200 mg/kg靜脈滴註0.5h,然後維持血MgSO4濃度分彆為7.0 mmol/L、8.0 mmol/L以及9.0 mmol/L.肺動脈、股動脈插管檢測平均肺動脈壓(MPAP)、體循環平均動脈壓(MAP),應用酶聯免疫吸附以及放免法分彆測定血清中白介素6(IL-6)和腫瘤壞死因子α(TNF-α),Western blot測定肺組織中單覈細胞趨化蛋白-1(MCP-1)蛋白錶達.結果 ①對照組MPAP較空白組顯著升高(P＜0.01),MgSO4 7.0組、MgSO48.0組以及MgSO49.0組MPAP較對照組顯著下降(P＜0.05或P＜0.01),其中MgSO49.0組下降最明顯；對照組MAP較空白組顯著降低(P ＜0.05或P＜0.01),MgSO4 7.0組、MgSO48.0組以及MgSO49.0組MAP較對照組顯著升高(P ＜0.05或P＜0.01),其中MgSO49.0組升高最明顯.②對照組血清中TNF-α以及IL-6較空白組升高(P＜0.05),MgSO4 7.0組、MgSO4 8.0組以及MgSO4 9.0組TNF-α以及IL-6較對照組顯著下降(P＜0.05或P＜0.01),MgSO4 9.0組下降最明顯；③對照組MCP-1蛋白錶達較空白組顯著升高(P＜0.01),MgSO4 7.0組、MgSO4 8.0組以及MgSO49.0組MCP-1蛋白錶達較對照組顯著下降(P＜0.01),MgSO49.0組蛋白錶達最低.結論 MgSO4可顯著降低肺動脈高壓,這可能與其抑製肺組織MCP-1錶達,降低TNF-α以及IL-6,從而抑製炎癥反應增彊有關.
목적 탐토류산미(MgSO4)대급성폐전새폐동맥고압적영향급기항염궤제.방법 30지웅성잡충견,체질량(15±2.1) kg,수궤분위5조:공백조、대조조이급MgSO47.0조、MgSO48.0조、MgSO4 9.0조,매조6지,기중공백조불급여임하처리；대조조조성급성폐동맥고압；기여삼조조성급성폐동맥고압후급여MgSO4정적,은정30 min후,MgSO4 200 mg/kg정맥적주0.5h,연후유지혈MgSO4농도분별위7.0 mmol/L、8.0 mmol/L이급9.0 mmol/L.폐동맥、고동맥삽관검측평균폐동맥압(MPAP)、체순배평균동맥압(MAP),응용매련면역흡부이급방면법분별측정혈청중백개소6(IL-6)화종류배사인자α(TNF-α),Western blot측정폐조직중단핵세포추화단백-1(MCP-1)단백표체.결과 ①대조조MPAP교공백조현저승고(P＜0.01),MgSO4 7.0조、MgSO48.0조이급MgSO49.0조MPAP교대조조현저하강(P＜0.05혹P＜0.01),기중MgSO49.0조하강최명현；대조조MAP교공백조현저강저(P ＜0.05혹P＜0.01),MgSO4 7.0조、MgSO48.0조이급MgSO49.0조MAP교대조조현저승고(P ＜0.05혹P＜0.01),기중MgSO49.0조승고최명현.②대조조혈청중TNF-α이급IL-6교공백조승고(P＜0.05),MgSO4 7.0조、MgSO4 8.0조이급MgSO4 9.0조TNF-α이급IL-6교대조조현저하강(P＜0.05혹P＜0.01),MgSO4 9.0조하강최명현；③대조조MCP-1단백표체교공백조현저승고(P＜0.01),MgSO4 7.0조、MgSO4 8.0조이급MgSO49.0조MCP-1단백표체교대조조현저하강(P＜0.01),MgSO49.0조단백표체최저.결론 MgSO4가현저강저폐동맥고압,저가능여기억제폐조직MCP-1표체,강저TNF-α이급IL-6,종이억제염증반응증강유관.
Objective To investigate the effect and the anti-inflammatory mechanism of magnesium sulfate (MgSO4) on acute pulmonary hypertension.Methods 30 mongrel dogs were randomly divided into five groups:control group,surgical group,MgSO4 7.0 group,MgSO4 8.0 group,and MgSO4 9.0 group.The control group was not given any treatment,and the surgical group was operated to induce acute pulmonary hypertension,and the three MgSO4 groups were given MgSO4 to maintain intravenous concentration with 7.0 mmol/L,8.0 mmol/L,and 9.0 mmol/L respectively after acute pulmonary hypertension.The mean pulmonary artery pressure (MPAP) and circulation mean arterial pressure (MAP) were detected.Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in serum were measured by enzyme-linked immunosorbent assay and radioimmunoassay,monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissue was determined by western blot.Results ①MPAP,TNF-α,IL-6,and MCP 1 protein expression were significantly higher in surgical group than those in control group,and were decreased significantly in MgSO4 7.0 group,MgSO4 8.0 group,and MgSO4 9.0 group with dose dependence.②MAP was significantly lower in surgical group than that in control group.MAP in MgSO4 7.0 group,MgSO4 8.0 group,and MgSO4 9.0 group was significantly increased compared with surgical group and MAP in MgSO4 9.0 group was the highest.Conclusions MgSO4 significantly reduces pulmonary hypertension,which is probably associated with reduction of the inflammatory response by inhibition of MCP-1 expression in lung tissue and decrease of TNF-α and IL-6 in serum.
