国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2013年
22期
1691-1695
,共5页
朱涛峰%陈如华%杨宏峰%冯艳%陆勤%虞浩%陈建强
硃濤峰%陳如華%楊宏峰%馮豔%陸勤%虞浩%陳建彊
주도봉%진여화%양굉봉%풍염%륙근%우호%진건강
香豆素类铜药物%肺癌%凋亡%Caspase-3
香豆素類銅藥物%肺癌%凋亡%Caspase-3
향두소류동약물%폐암%조망%Caspase-3
Coumarin-copper drugs%Lung cancer%Apoptosis%Caspase-3
目的 研究香豆素缩氨基酸型曼尼西碱金属香豆素类铜药物(简称香豆素类铜药物)在体内外抗肺腺癌A549细胞的生物学活性.方法 不同浓度香豆素类铜药物干预A549细胞,用MTT法、Annexin V/PI及Western blot进行检测体外抗肺癌活性.建立A549荷瘤裸鼠模型后,经过香豆素类铜药物的干预,绘制肿瘤生长曲线并计算肿瘤干预的抑瘤率,通过TUNEL法检测体内肿瘤细胞凋亡.结果 香豆素类铜药物以浓度时间依赖性方式有效地抑制A549细胞增殖,抑制A549细胞活力的IC50为2.0μmol/L;香豆素类铜药物能以浓度依赖性方式诱导A549细胞凋亡(P＜0.05);伴有caspase-3蛋白表达上调.香豆素类铜药物体内以浓度依赖性方式抑制肺癌A549移植瘤生长(P＜0.05),且凋亡明显增多(P＜0.05).结论 香豆素类铜药物对肺腺癌A549的体内外抑制生长作用均很明显,且可能通过caspase途径诱导细胞凋亡起作用.
目的 研究香豆素縮氨基痠型曼尼西堿金屬香豆素類銅藥物(簡稱香豆素類銅藥物)在體內外抗肺腺癌A549細胞的生物學活性.方法 不同濃度香豆素類銅藥物榦預A549細胞,用MTT法、Annexin V/PI及Western blot進行檢測體外抗肺癌活性.建立A549荷瘤裸鼠模型後,經過香豆素類銅藥物的榦預,繪製腫瘤生長麯線併計算腫瘤榦預的抑瘤率,通過TUNEL法檢測體內腫瘤細胞凋亡.結果 香豆素類銅藥物以濃度時間依賴性方式有效地抑製A549細胞增殖,抑製A549細胞活力的IC50為2.0μmol/L;香豆素類銅藥物能以濃度依賴性方式誘導A549細胞凋亡(P＜0.05);伴有caspase-3蛋白錶達上調.香豆素類銅藥物體內以濃度依賴性方式抑製肺癌A549移植瘤生長(P＜0.05),且凋亡明顯增多(P＜0.05).結論 香豆素類銅藥物對肺腺癌A549的體內外抑製生長作用均很明顯,且可能通過caspase途徑誘導細胞凋亡起作用.
목적 연구향두소축안기산형만니서감금속향두소류동약물(간칭향두소류동약물)재체내외항폐선암A549세포적생물학활성.방법 불동농도향두소류동약물간예A549세포,용MTT법、Annexin V/PI급Western blot진행검측체외항폐암활성.건립A549하류라서모형후,경과향두소류동약물적간예,회제종류생장곡선병계산종류간예적억류솔,통과TUNEL법검측체내종류세포조망.결과 향두소류동약물이농도시간의뢰성방식유효지억제A549세포증식,억제A549세포활력적IC50위2.0μmol/L;향두소류동약물능이농도의뢰성방식유도A549세포조망(P＜0.05);반유caspase-3단백표체상조.향두소류동약물체내이농도의뢰성방식억제폐암A549이식류생장(P＜0.05),차조망명현증다(P＜0.05).결론 향두소류동약물대폐선암A549적체내외억제생장작용균흔명현,차가능통과caspase도경유도세포조망기작용.
Objective To investigate effect of the copper (Ⅰ) complexes with coumarin derivatives (coumarin-copper drugs) on lung cancer cell (A549 cell line) in vivo and vitro and its mechanism of action.Methods A549 cells were treated with different concentrations of the coumarin-copper drugs,MTT assay was performed to determine cell ratio.Cell apoptosis was detected by Annexin V/PI flow cytometry.Western blot was performed to evaluate the protein expression of caspase-3.The subcutaneous transplantable tumor model of A549 cell in nude mice was established.The nude mice in each group received corresponding treated.The growth curves of the tumor were drawn and the tumor growth inhibition rates were calculated.The apoptosis index of subcutaneously transplanted tumor cells was served by TUNEL.Results Coumarin-copper drugs effectively inhibited the proliferation of A549 in a dose-and-time dependent manner,and the IC50 was 2.0 μmol/L.Coumarin-copper drugs could induce the apoptosis of A549 cells in a dose dependent way (P ＜ 0.05),which was accompany with upregulation of caspase-3.Coumarin copper drugs effectively inhibited the growth of A549 tumor in a dose dependent manner,kept company with the apoptosis index of A549 tumor (P ＜0.05).Conclusions Coumarin copper drugs may inhibit the proliferation of A549 cells through inducing cell apoptosis,which should be related to the up-regulation of caspase-3.
