CAJ | 학술논문

目的检测COPD患者血清中25-羟维生素D[25-hydroxyvitamin D,25-(OH)D]与基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的表达水平并探讨两者之间的关系.方法收集2012年1月至2012年3月在长沙市第三医院呼吸内科住院的38例COPD急性加重期患者(COPD急性加重组)和同期同院门诊随诊的40例COPD稳定期患者(COPD稳定组),同时选择30例在同院体检中心健康体检者(对照组).采用ELISA法测定并比较3组受试者血清25-(OH)D和MMP-9的表达水平,分别探讨COPD稳定组和急性加重组中血清25-(OH)D和MMP-9水平之间的关系.结果 ①3组的血清25-(OH)D水平比较,COPD急性加重组和COPD稳定组分别低于对照组(F=86.63,P＜0.01),COPD急性加重组低于COPD稳定组(F=86.63,P＜0.05);3组的血清MMP-9水平比较,COPD急性加重组和COPD稳定组分别高于对照组(F=436.02,P＜0.01),COPD急性加重组高于COPD稳定组(F =436.02,P＜0.01).②COPD稳定组25-(OH)D与MMP-9水平呈负相关(r=-0.638,P＜0.01).COPD急性加重组25-(OH)D与MMP-9水平呈负相关(r=-0.579,P＜0.01).结论维生素D在COPD中呈低表达而MMP-9呈高表达.在COPD患者中,维生素D水平可能会影响MMP-9的表达.
목적 검측COPD환자혈청중25-간유생소D[25-hydroxyvitamin D,25-(OH)D]여기질금속단백매-9(matrix metalloproteinase-9,MMP-9)적표체수평병탐토량자지간적관계.방법 수집2012년1월지2012년3월재장사시제삼의원호흡내과주원적38례COPD급성가중기환자(COPD급성가중조)화동기동원문진수진적40례COPD은정기환자(COPD은정조),동시선택30례재동원체검중심건강체검자(대조조).채용ELISA법측정병비교3조수시자혈청25-(OH)D화MMP-9적표체수평,분별탐토COPD은정조화급성가중조중혈청25-(OH)D화MMP-9수평지간적관계.결과 ①3조적혈청25-(OH)D수평비교,COPD급성가중조화COPD은정조분별저우대조조(F=86.63,P＜0.01),COPD급성가중조저우COPD은정조(F=86.63,P＜0.05);3조적혈청MMP-9수평비교,COPD급성가중조화COPD은정조분별고우대조조(F=436.02,P＜0.01),COPD급성가중조고우COPD은정조(F =436.02,P＜0.01).②COPD은정조25-(OH)D여MMP-9수평정부상관(r=-0.638,P＜0.01).COPD급성가중조25-(OH)D여MMP-9수평정부상관(r=-0.579,P＜0.01).결론 유생소D재COPD중정저표체이MMP-9정고표체.재COPD환자중,유생소D수평가능회영향MMP-9적표체.
Objective To detect the levels of 25-hydroxyvitamin D [25-(OH)D] and matrix metalloproteinase-9 (MMP-9) in COPD and to analyze the correlations of these indices.Methods Thirty-eight patients with acute exacerbations of COPD (AECOPD group),40 outpatients with stable COPD (stable COPD group) and 30 healthy subjects(control group) in the Third Hospital of Changsha were enrolled in our study from Jan.2012 to Mar.2012.Serum levels of 25-(OH)D and MMP-9 were measured in all subjects by the ELISA and compared among three groups.Correlations between 25-(OH)D and MMP-9 were analyzed in stable COPD group and AECOPD group.Results ①The levels of 25-(OH)D in AECOPD group and stable COPD group were significantly lower than those in control group (F =86.63,P ＜0.01).The level of 25-(OH)D in AECOPD group was significantly lower than that in stable COPD group (F =86.63,P ＜0.05).The levels of MMP-9 in AECOPD group and stable COPD group were significantly higher than those in control group (F =436.02,P ＜ 0.01).The level of MMP-9 in AECOPD group was significantly higher than that in stable COPD group (F =436.02,P ＜0.01).② Both in stable COPD group and AECOPD group,the 25-(OH)D levels were negatively correlated with the MMP-9 levels respectively (r =-0.638,P ＜0.01.r =-0.579,P ＜0.01,respectively).Conclusions Vitamin D in COPD showed lower expression,while higher expression of MMP-9.Vitamin D may affect the expression of MMP-9 in COPD.