国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2014年
10期
732-735
,共4页
梁艳%吴雪琼%阳幼荣%张俊仙
樑豔%吳雪瓊%暘幼榮%張俊仙
량염%오설경%양유영%장준선
结核分枝杆菌%Ag85A%Ag85B%蛋白%治疗
結覈分枝桿菌%Ag85A%Ag85B%蛋白%治療
결핵분지간균%Ag85A%Ag85B%단백%치료
Mycobacterium tuberculosis%Ag85A%Ag85B%Protein%Therapy
目的 研究结核分枝杆菌Ag85A和Ag85B重组蛋白疫苗联合佐剂对结核的治疗作用.方法 用结核分枝杆菌H37Rv通过尾静脉注射60只BALB/c小鼠,分别用生理盐水、利福平、草分枝杆菌F.U.36注射液、佐剂77-1、Ag85A和Ag85B重组蛋白疫苗、Ag85A和Ag85B重组蛋白疫苗联合佐剂治疗.免疫结束后2周杀鼠,观察肺和脾病理改变,称取重量,做菌落计数.结果 肺组织病理显示:生理盐水组肺组织病变严重、广泛,佐剂组比生理盐水组病变略轻,病变仍较重、广泛,草分枝杆菌F.U.36注射液组、Ag85A和Ag85B重组蛋白疫苗组、Ag85A和Ag85B重组蛋白疫苗联合佐剂组肺组织病变减轻、局限,3/5区域肺泡结构完整、清晰,利福平组肺组织无病变.与生理盐水组比较,利福平组、草分枝杆菌F.U.36注射液组、Ag85A和Ag85B重组蛋白疫苗联合佐剂组肺脏菌落数分别减少2.11 log、0.76 log和0.57 log;肝脏菌落数分别减少2.11 log、0.74 log和0.44 log(t＞3.21,P＜0.01).结论 Ag85A和Ag85B重组蛋白疫苗联合佐剂对小鼠结核病具有较好的治疗效果,与草分枝杆菌F.U.36注射液相当.
目的 研究結覈分枝桿菌Ag85A和Ag85B重組蛋白疫苗聯閤佐劑對結覈的治療作用.方法 用結覈分枝桿菌H37Rv通過尾靜脈註射60隻BALB/c小鼠,分彆用生理鹽水、利福平、草分枝桿菌F.U.36註射液、佐劑77-1、Ag85A和Ag85B重組蛋白疫苗、Ag85A和Ag85B重組蛋白疫苗聯閤佐劑治療.免疫結束後2週殺鼠,觀察肺和脾病理改變,稱取重量,做菌落計數.結果 肺組織病理顯示:生理鹽水組肺組織病變嚴重、廣汎,佐劑組比生理鹽水組病變略輕,病變仍較重、廣汎,草分枝桿菌F.U.36註射液組、Ag85A和Ag85B重組蛋白疫苗組、Ag85A和Ag85B重組蛋白疫苗聯閤佐劑組肺組織病變減輕、跼限,3/5區域肺泡結構完整、清晰,利福平組肺組織無病變.與生理鹽水組比較,利福平組、草分枝桿菌F.U.36註射液組、Ag85A和Ag85B重組蛋白疫苗聯閤佐劑組肺髒菌落數分彆減少2.11 log、0.76 log和0.57 log;肝髒菌落數分彆減少2.11 log、0.74 log和0.44 log(t＞3.21,P＜0.01).結論 Ag85A和Ag85B重組蛋白疫苗聯閤佐劑對小鼠結覈病具有較好的治療效果,與草分枝桿菌F.U.36註射液相噹.
목적 연구결핵분지간균Ag85A화Ag85B중조단백역묘연합좌제대결핵적치료작용.방법 용결핵분지간균H37Rv통과미정맥주사60지BALB/c소서,분별용생리염수、리복평、초분지간균F.U.36주사액、좌제77-1、Ag85A화Ag85B중조단백역묘、Ag85A화Ag85B중조단백역묘연합좌제치료.면역결속후2주살서,관찰폐화비병리개변,칭취중량,주균락계수.결과 폐조직병리현시:생리염수조폐조직병변엄중、엄범,좌제조비생리염수조병변략경,병변잉교중、엄범,초분지간균F.U.36주사액조、Ag85A화Ag85B중조단백역묘조、Ag85A화Ag85B중조단백역묘연합좌제조폐조직병변감경、국한,3/5구역폐포결구완정、청석,리복평조폐조직무병변.여생리염수조비교,리복평조、초분지간균F.U.36주사액조、Ag85A화Ag85B중조단백역묘연합좌제조폐장균락수분별감소2.11 log、0.76 log화0.57 log;간장균락수분별감소2.11 log、0.74 log화0.44 log(t＞3.21,P＜0.01).결론 Ag85A화Ag85B중조단백역묘연합좌제대소서결핵병구유교호적치료효과,여초분지간균F.U.36주사액상당.
Objective To evaluate the therapeutic effects of Ag85A and Ag85B recombination protein vaccines combined with adjuvant in mouse model of Mycobacterium tuberculosis infection to establish new immunotherapeutic agents to treat tuberculosis.Methods 60 female BALB/c mice were infected intravenously via the tail vein with Mycobacterium tuberculosis H37RV,then randomly divided into six groups based and treated as follow at the third day after infection:saline,rifampin,Mycobacterium Phlei F.U.36 injection,adjuvant,Ag85A and Ag85B recombinat protein vaccines,Ag85A and Ag85B recombinat protein vaccines combined with adjuvant.Vaccines were injected intramuscularly three times at two-week intervals.Mice were sacrificed two weeks after the final immunization.The lungs and spleens from the mice were taken and their pathological changes,weight and number of mycobacterial colony were examined.Results The histopathological changes of lung showed that the lung lesions were severe and extensive in saline and adjuvant group,the lung lesions were slight and limited,there were relatively clearand normal structure of alveoli in Mycobacterium Phlei F.U.36 injection,adjuvant,Ag85A and Ag85B recombinatprotein vaccines,Ag85A and Ag85B recombinat protein vaccines combined with adjuvant group,there were no lung lesions in rifampin group.Compared with saline group,pulmonary bacterial loads of rifampin group,Mycobacterium Phlei F.U.36 injection group,Ag85A and Ag85B recombinat protein vaccines combined with adjuvant group reduced by 2.11 log,0.76 log,and 0.57 log,liver bacterial loads by 2.11 log,0.74 log,and 0.44 log (t ＞ 3.21,P ＜ 0.01),respectively.Conclusions The immunotherapeutic effect of Ag85A and Ag85B recombinat protein vaccines combined with adjuvant is significant in the mouse model of tuberculosis,as that as Mycobacterium Phlei F.U.36 injection.
