国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2013年
1期
6-9
,共4页
郑培明%乔铁%马瑞红%蔡洪英%罗小兵%罗振亮%杨柳青
鄭培明%喬鐵%馬瑞紅%蔡洪英%囉小兵%囉振亮%楊柳青
정배명%교철%마서홍%채홍영%라소병%라진량%양류청
胆囊结石%胆囊壁%华支睾吸虫%基因检测%实时荧光PCR
膽囊結石%膽囊壁%華支睪吸蟲%基因檢測%實時熒光PCR
담낭결석%담낭벽%화지고흡충%기인검측%실시형광PCR
Cholecystolithiasis%Gallbladder wall%Clonorchis sinensis%Gene detection%Real-time PCR
目的 运用实时荧光PCR技术检测胆囊结石患者胆囊壁中的华支睾吸虫DNA,并评估其检测效价.方法 随机选取60例胆囊结石患者的胆囊壁、胆汁及胆石标本,运用实时荧光PCR技术对3种标本分别进行华支睾吸虫DNA检测,另取部分胆囊壁标本进行常规病理检查.结果 60例胆囊结石患者的胆囊壁、胆汁及胆石标本中,实时荧光PCR检测华支睾吸虫DNA阳性率分别为51.67％ (31/60)、56.67％(34/60)和60.00％ (36/60),3组标本相比差异无统计学意义(x2=0.857,P＞0.05).胆囊壁病理切片检查虫卵阳性率为8.33％(5/60),与以上3种方法相比,差异有统计学意义(x2=42.512,P＜0.01).结论 在胆囊结石患者胆囊壁组织中发现华支睾吸虫卵,实时荧光PCR能检测到华支睾吸虫DNA.
目的 運用實時熒光PCR技術檢測膽囊結石患者膽囊壁中的華支睪吸蟲DNA,併評估其檢測效價.方法 隨機選取60例膽囊結石患者的膽囊壁、膽汁及膽石標本,運用實時熒光PCR技術對3種標本分彆進行華支睪吸蟲DNA檢測,另取部分膽囊壁標本進行常規病理檢查.結果 60例膽囊結石患者的膽囊壁、膽汁及膽石標本中,實時熒光PCR檢測華支睪吸蟲DNA暘性率分彆為51.67％ (31/60)、56.67％(34/60)和60.00％ (36/60),3組標本相比差異無統計學意義(x2=0.857,P＞0.05).膽囊壁病理切片檢查蟲卵暘性率為8.33％(5/60),與以上3種方法相比,差異有統計學意義(x2=42.512,P＜0.01).結論 在膽囊結石患者膽囊壁組織中髮現華支睪吸蟲卵,實時熒光PCR能檢測到華支睪吸蟲DNA.
목적 운용실시형광PCR기술검측담낭결석환자담낭벽중적화지고흡충DNA,병평고기검측효개.방법 수궤선취60례담낭결석환자적담낭벽、담즙급담석표본,운용실시형광PCR기술대3충표본분별진행화지고흡충DNA검측,령취부분담낭벽표본진행상규병리검사.결과 60례담낭결석환자적담낭벽、담즙급담석표본중,실시형광PCR검측화지고흡충DNA양성솔분별위51.67％ (31/60)、56.67％(34/60)화60.00％ (36/60),3조표본상비차이무통계학의의(x2=0.857,P＞0.05).담낭벽병리절편검사충란양성솔위8.33％(5/60),여이상3충방법상비,차이유통계학의의(x2=42.512,P＜0.01).결론 재담낭결석환자담낭벽조직중발현화지고흡충란,실시형광PCR능검측도화지고흡충DNA.
Objective To detect Clonorchis sinensis DNA in the gallbladder wall of patients with cholecystolithiasis by a TaqMan based real-time PCR and to evaluation its value for detection.Methods Sixty patients with cholecystolithiasis were randomly selected from those who had accepted endoscopic gallbladderpreserving cholelithotomy from March to May 2012.Three types of samples (i.e.gallbladder wall,bile and gallstones) were obtained from the operation and used for DNA extraction,then a real-time PCR assay was developed for the detection of C.sinensis DNA.The gallbladder walls were also used for pathological examination.Results The positive rates of PCR amplification of C.sinensis DNA in the three samples were 51.67％ (31/60),56.67％ (34/60) and 60.00％ (36/60),respectively,and there was no significant difference among three groups (x2 =0.857,P ＞ 0.05).The C.sinensis eggs were found in five gallbladder wall samples with the positive rate 8.33％(5/60),which was significant lower than the results of above three methods (x2 =42.512,P ＜0.01).Conclusion C.sinensis DNA was detected in gallbladder wall through real-time PCR and C.sinensis eggs were found in the pathological sections of gallbladder wall.
