CAJ | 학술논문

目的培育伯氏疟原虫K173哌喹抗性株.方法参照大剂量复燃法进行抗哌喹虫株的培育,每5代用Peters 4天抑制试验检测90％有效剂量(90％ effective dose,ED90)并计算抗性指数(resistance in-dex,I)I90.结合Artekin等抗性培育资料,比较哌喹和磷酸哌喹的抗药性.结果实验历时7个多月,培育至30代,哌喹抗性株抗性指数I90为22.9,属于中度抗性.Artekin资料中,培育至第30代,磷酸哌喹抗性株抗性指数I90为371.1,属于重度抗性；Artequick药效学资料中,培育至第30代,哌喹抗性株抗性指数I90为27.6,属于中度抗性.结论哌喹较易产生抗药性,但其抗性比磷酸哌喹轻,抗性产生时间也较磷酸哌喹迟.
목적 배육백씨학원충K173고규항성주.방법 삼조대제량복연법진행항고규충주적배육,매5대용Peters 4천억제시험검측90％유효제량(90％ effective dose,ED90)병계산항성지수(resistance in-dex,I)I90.결합Artekin등항성배육자료,비교고규화린산고규적항약성.결과 실험력시7개다월,배육지30대,고규항성주항성지수I90위22.9,속우중도항성.Artekin자료중,배육지제30대,린산고규항성주항성지수I90위371.1,속우중도항성；Artequick약효학자료중,배육지제30대,고규항성주항성지수I90위27.6,속우중도항성.결론 고규교역산생항약성,단기항성비린산고규경,항성산생시간야교린산고규지.
Objective To induce the resistance to piperaquine in a line of Plasmodium berghei K173.Method The 2％ relapse technique was used for cultivation.Peters' 4-day suppressive test was performed to detect the drug's 90％ effective dose(ED90) in order to calculate the resistance index I90.The resistance of piperaquine and piperaquine phosphate was compared with the statistics in Artekin et al.Results By cultivation to 30 passage,the I90 of piperaquine was 22.9 belonging to moderate resistance.Data in Artekin showed that at 30 passage,the I90 of piperaquine phosphate was 371.1 belonging to heavy resistance.While data in Artequick indicated that the I90 of piperaquine was 27.6 belonging to moderate resistance at 30 passage.Conclusion Piperaquine was easy to become drug resistant,but the resistance was much milder than piperaquine phosphate,and the occurrence time of the resistance was slower than piperaquine phosphate.