CAJ | 학술논문

目的观察三苯双脒对感染华支睾吸虫仓鼠的疗效及其对华支睾吸虫成虫超微结构的影响. 方法药效实验:99只仓鼠各感染30条华支睾吸虫囊蚴,分组后灌胃顿服给药治疗,观察各组疗效:1)59只仓鼠于感染后24 d随机分成7组,每组8～9只,分别为三苯双脒50.0、100.0和200.0 mg/kg组,吡喹酮100.0、200.0和300.0 mg/kg组以及对照组；2)40只仓鼠感染后24 d随机分成5组,每组8只,分别为三苯双脒12.5和25.0 mg/kg组,吡喹酮50.0和75.0 mg/kg组以及对照组.各组受治鼠于治疗后2周剖杀,收集胆道系统内的残留华支睾吸虫,计算各组的平均虫数和减虫率.超微结构观察实验:每只大鼠灌胃50条华支睾吸虫囊蚴6周后,分为治疗组和对照组:其中治疗组10只,经三苯双脒300.0mg/kg治疗后,分别于4、8、24、48和72h剖杀大鼠,从胆总管中检获华支睾吸虫；对照组10只,于相同时间点剖杀取虫.检获的华支睾吸虫经处理后,置于透射电镜下观察. 结果三苯双脒12.5、25.0、50.0、100.0和200.0 mg/kg组的减虫率分别为50.0％、89.7％、95.7％、99.3％和100％,平均虫数与对照组相比差异均有统计学意义.吡喹酮50.0、75.0、100.0、200.0和300.0 mg/kg组的减虫率分别为53.1％、78.6％、81.5％、88.1％和100％.经三苯双脒作用后,大鼠体内华支睾吸虫成虫体壁皮层和皮层细胞内杆状颗粒、盘状颗粒和膜样小泡减少,皮层内线粒体出现空泡,皮层乳突破溃剥落,皮层细胞核变形,核膜消失. 结论三苯双脒对华支睾吸虫感染鼠有较好疗效,且优于吡喹酮.三苯双脒可引起华支睾吸虫体壁皮层和皮层细胞亚结构破坏.
목적 관찰삼분쌍미대감염화지고흡충창서적료효급기대화지고흡충성충초미결구적영향. 방법 약효실험:99지창서각감염30조화지고흡충낭유,분조후관위돈복급약치료,관찰각조료효:1)59지창서우감염후24 d수궤분성7조,매조8～9지,분별위삼분쌍미50.0、100.0화200.0 mg/kg조,필규동100.0、200.0화300.0 mg/kg조이급대조조；2)40지창서감염후24 d수궤분성5조,매조8지,분별위삼분쌍미12.5화25.0 mg/kg조,필규동50.0화75.0 mg/kg조이급대조조.각조수치서우치료후2주부살,수집담도계통내적잔류화지고흡충,계산각조적평균충수화감충솔.초미결구관찰실험:매지대서관위50조화지고흡충낭유6주후,분위치료조화대조조:기중치료조10지,경삼분쌍미300.0mg/kg치료후,분별우4、8、24、48화72h부살대서,종담총관중검획화지고흡충；대조조10지,우상동시간점부살취충.검획적화지고흡충경처리후,치우투사전경하관찰. 결과 삼분쌍미12.5、25.0、50.0、100.0화200.0 mg/kg조적감충솔분별위50.0％、89.7％、95.7％、99.3％화100％,평균충수여대조조상비차이균유통계학의의.필규동50.0、75.0、100.0、200.0화300.0 mg/kg조적감충솔분별위53.1％、78.6％、81.5％、88.1％화100％.경삼분쌍미작용후,대서체내화지고흡충성충체벽피층화피층세포내간상과립、반상과립화막양소포감소,피층내선립체출현공포,피층유돌파궤박락,피층세포핵변형,핵막소실. 결론 삼분쌍미대화지고흡충감염서유교호료효,차우우필규동.삼분쌍미가인기화지고흡충체벽피층화피층세포아결구파배.
Objective To observe the efficacy of tribendimidine on hamsters infected with Clonorchis sinensis and the ultrastructural changes of the adult worm after tribendimidine treatment.Methods In the experiment of drug efficacy,a total of 99 hamsters each infected with 30 C.sinensis metacercariae were administrated with tribendimidine and praziquantel to observe the efficacy in following two aspects:1) 59 infected hamsters were randomly divided into 7 groups with 8 or 9 per group at the 24th day after infection:tribendimidine groups of 50.0,100.0,200.0 mg/kg and praziquantel groups of 100.0,200.0,300.0 mg/kg and the control group; 2) 40 infected hamsters were randomly divided into 5 groups:tribendimidine 12.5 and 25.0 mg/kg,praziquantel 50.0 and 75.0 mg/kg and the control group.Each hamster was killed 2 weeks after treatment,C.sinensis was collected from the bile ducts and mean worm burden and mean worm reduction rate were calculated.In the experiment on the ultrastructural changes of adult worm,rats infected with 50 C.sinensis metacercariae were divided into two groups 6 weeks after infection:one group of 10 rats were treated with tribendimidine 300.0 mg/kg and killed at 4,8,24,48 and 72 h after treatment; 10 rats of control group were killed at the same time points.C.sinensis was collected from the bile ducts and observed under transmission electron microscope.Results The worm reduction rates in tribendimidine 12.5,25.0,50.0,100.0 and 200.0 mg/kg group were 50.0％,89.7％,95.7％,99.3％ and 100％ respectively,significant difference was found in mean worm burden compared with that in control group.The worm reduction rates in praziquantel 50.0,75.0,100.0,200.0 and 300.0 mg/kg group were 53.1％,78.6％,81.5％,88.1％ and 100％,respectively.The rod-like granules,disc-like granules and vacuoles in the integument and integument cells of C.sinensis decreased after tribendimidine treatment,and the papillae of integument dissolved and stripped,the vacuolization of mitochondria in the integument appeared,the integument cell nucleus deformed,and parts of cell nuclear membrane dissolved.Conclusions Tribendimidine exhibites good efficacy to hamsters infected with C.sinensis,which is superior to praziquantel.The ultrastructure of integument and integument cells in body of C.sinensis were deformed after tribendimidine treatment.