CAJ | 학술논문

目的了解人类免疫缺陷病毒(human immunodeficiency virus,HIV)与弓形虫双重感染者的一氧化氮(nitric oxide,NO)水平及感染弓形虫与NO之间的关系. 方法收集144例HIV阳性者血清,进行弓形虫抗体检测,于9个月后再次取血,前后两次取得的血清应用硝酸还原酶法测定其NO的水平;对HIV阳性者血清进行CD4+细胞计数,根据CD4+细胞计数水平将患者分为免疫功能正常组、免疫功能受损组和免疫功能严重受损组,检测三组患者的NO水平.用SPSS 16.0软件对检测结果进行统计学分析. 结果 HIV与弓形虫双重者感染者的血清NO值为(82.77±5.82) μmol/L,HIV阳性无弓形虫感染者的NO值为(44.56±5.38) μmol/L,二者差异有统计学意义(P＜0.05);第一次和第二次检测的HIV与弓形虫双重感染者NO水平分别为(81.37±8.22)μmol/L和(84.18±8.28) μmol/L,差异无统计学意义(P＞0.05);第一次和第二次检测的HIV单一感染者NO值分别为(48.35±9.16) μmol/L和(40.78±5.67)μmol/L,差异无统计学意义(P＞0.05).免疫功能正常组NO水平与免疫功能受损组、免疫功能严重受损组进行比较,差异均具有统计学意义(P＜0.05);免疫功能受损组NO水平高于免疫功能严重受损组,但差异无统计学意义(P＞0.05). 结论 HIV与弓形虫双重感染者血清NO水平比HIV阳性单一阳性者高,随着HIV阳性者CD4+细胞计数的下降,机体产生NO的能力也在减弱.
목적 료해인류면역결함병독(human immunodeficiency virus,HIV)여궁형충쌍중감염자적일양화담(nitric oxide,NO)수평급감염궁형충여NO지간적관계. 방법 수집144례HIV양성자혈청,진행궁형충항체검측,우9개월후재차취혈,전후량차취득적혈청응용초산환원매법측정기NO적수평;대HIV양성자혈청진행CD4+세포계수,근거CD4+세포계수수평장환자분위면역공능정상조、면역공능수손조화면역공능엄중수손조,검측삼조환자적NO수평.용SPSS 16.0연건대검측결과진행통계학분석. 결과 HIV여궁형충쌍중자감염자적혈청NO치위(82.77±5.82) μmol/L,HIV양성무궁형충감염자적NO치위(44.56±5.38) μmol/L,이자차이유통계학의의(P＜0.05);제일차화제이차검측적HIV여궁형충쌍중감염자NO수평분별위(81.37±8.22)μmol/L화(84.18±8.28) μmol/L,차이무통계학의의(P＞0.05);제일차화제이차검측적HIV단일감염자NO치분별위(48.35±9.16) μmol/L화(40.78±5.67)μmol/L,차이무통계학의의(P＞0.05).면역공능정상조NO수평여면역공능수손조、면역공능엄중수손조진행비교,차이균구유통계학의의(P＜0.05);면역공능수손조NO수평고우면역공능엄중수손조,단차이무통계학의의(P＞0.05). 결론 HIV여궁형충쌍중감염자혈청NO수평비HIV양성단일양성자고,수착HIV양성자CD4+세포계수적하강,궤체산생NO적능력야재감약.
Objective To understand the nitric oxide(NO) levels in human immunodeficiency virus (HIV)positive patients infected with Toxoplasma,and the relationship between Toxoplasma infection and NO.Methods Antibody against Toxoplasma was detected in 144 sera samples collected from HIV positive patientsand the detection was repeated after nine months.The method of nitrate reductive enzyme was applied for deter-mination of the levels of NO in all sera samples.CD4+ cells were counted in all HIV positive sera which werethen divided into three groups:normal immune function group,impaired immune function group and immunefunction severely damaged group according to the CD4+ cell level.The NO levels in three groups were statistical-ly analysed using SPSS16.0 software.Results The NO value (82.77 ± 5.82) μmol/L in T.gondii infection serawas significantly higher than that in non-infection with T.gondii sera(44.56 ± 5.38) μmol/L(P＜0.05).The firsttime and second time detected NO levels in HIV/Toxoplasma dual infection sera were (81.37+8.22) μmol/L and(84.18+8.28) μmol/L respectively,with no significant difference,while they were (48.35+9.16) μmol/L and(40.78+5.67) μmol/L in HIV single infection sera respectively,with no significant difference too.The differenceof NO level between normal immune function group and immune function impaired group or severely damagegroup was statistically significant (P＜0.05).The NO level in immune function impaired group is higher than the level in immune function severely damage group,but the difference was not statistically significant (P＞0.05).Concluslon The serum NO level in people dually infected with HIV and Toxoplasma is higher than that in people only infected with HIV.As the HIV positive CD4+ cell count goes down,the ability of the body to produce NO is weakened.