CAJ | 학술논문

核酸扩增是最为重要的生物技术之一.目前,PCR是应用范围最广的体外核酸扩增技术,但该技术需要温度循环仪器才能扩增目的基因,因此限制了其在基层和现场的应用.随着分子生物学技术的发展及其与相关交叉学科的结合,新的扩增技术相继出现,其中以恒温扩增技术为主导.按是否直接扩增目的基因,恒温扩增技术可分为靶核酸直接扩增技术和信号放大技术.该文就恒温扩增技术及其在病原生物学中的应用作一综述.
핵산확증시최위중요적생물기술지일.목전,PCR시응용범위최엄적체외핵산확증기술,단해기술수요온도순배의기재능확증목적기인,인차한제료기재기층화현장적응용.수착분자생물학기술적발전급기여상관교차학과적결합,신적확증기술상계출현,기중이항온확증기술위주도.안시부직접확증목적기인,항온확증기술가분위파핵산직접확증기술화신호방대기술.해문취항온확증기술급기재병원생물학중적응용작일종술.
Nucleic acid amplification is one of the most important technologies used in the field of molecular biology.So far PCR is the most widely used method.However,it requires a thermocycling machine to amplify the required fragment,which limits its application in field as a basic detecting means.Novel development of molecular biology and its combination with other disciplines make it possible for nucleic acid amplification in isothermal condition.Several isothermal amplification technologies emerged over the last few years and these methods can be classified into direct amplification technologies and signal amplification systems based on whether they directly amplify the target genes.These technologies and their applications in pathogen biological research were reviewed in this paper.Characteristics of each method and the prospect of these technologies were also discussed.