国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2014年
2期
83-89
,共7页
朱明星%王娅娜%巨艳%王志昇%朱佳佳%赵巍
硃明星%王婭娜%巨豔%王誌昇%硃佳佳%趙巍
주명성%왕아나%거염%왕지승%주가가%조외
细粒棘球蚴原头节%转录组%高通量转录组测序%生物信息学分析
細粒棘毬蚴原頭節%轉錄組%高通量轉錄組測序%生物信息學分析
세립극구유원두절%전록조%고통량전록조측서%생물신식학분석
Echinococcus granulosus protoscolex%Transcriptome%RNA-Seq%Bioinformatics analysis
目的 利用RNA-seq技术对细粒棘球蚴原头节的转录组进行测序并对测序数据进行生物信息学分析,以揭示细粒棘球蚴原头节mRNA所包含的信息. 方法 用Trizol法提取原头节总RNA,分离mRNA,利用Illumina公司的HiSeq2000高通量测序仪对mRNA序列进行测序.参考英国桑格研究院(Wellcome Trust Sanger Institute)公布的细粒棘球绦虫基因组数据,对测序数据进行拼接组装,将获得的unigene与非冗余的蛋白序列数据库(non-redundant,Nr)、全球蛋白资源数据库(universal protein,UniProt)、基因本体数据库(gene ontology,GO)以及日本京都基因和基因组百科全书通路数据库(the kyotoencyclopedia of genes and genomes pathway,KEGG pathway)进行比对,获得该unigene的蛋白功能注释信息. 结果 测序后共获得132 007 609条读段(reads),经过组装共形成91 342条unigene,平均长度为419 bp.通过GO分类注释,共有36 552个unigene获得了注释信息,分别归入到了生物学过程、分子功能、细胞组分3大类共计48个功能组.通过KEGG pathway的注释,有6 664条unigene注释到了KEGG上,参与了6大类生物过程中34个小类的代谢,共有227个代谢通路. 结论 获得了细粒棘球蚴原头节转录组相关信息,为棘球蚴病的后续研究提供了研究数据,积累了研究资料.
目的 利用RNA-seq技術對細粒棘毬蚴原頭節的轉錄組進行測序併對測序數據進行生物信息學分析,以揭示細粒棘毬蚴原頭節mRNA所包含的信息. 方法 用Trizol法提取原頭節總RNA,分離mRNA,利用Illumina公司的HiSeq2000高通量測序儀對mRNA序列進行測序.參攷英國桑格研究院(Wellcome Trust Sanger Institute)公佈的細粒棘毬縚蟲基因組數據,對測序數據進行拼接組裝,將穫得的unigene與非冗餘的蛋白序列數據庫(non-redundant,Nr)、全毬蛋白資源數據庫(universal protein,UniProt)、基因本體數據庫(gene ontology,GO)以及日本京都基因和基因組百科全書通路數據庫(the kyotoencyclopedia of genes and genomes pathway,KEGG pathway)進行比對,穫得該unigene的蛋白功能註釋信息. 結果 測序後共穫得132 007 609條讀段(reads),經過組裝共形成91 342條unigene,平均長度為419 bp.通過GO分類註釋,共有36 552箇unigene穫得瞭註釋信息,分彆歸入到瞭生物學過程、分子功能、細胞組分3大類共計48箇功能組.通過KEGG pathway的註釋,有6 664條unigene註釋到瞭KEGG上,參與瞭6大類生物過程中34箇小類的代謝,共有227箇代謝通路. 結論 穫得瞭細粒棘毬蚴原頭節轉錄組相關信息,為棘毬蚴病的後續研究提供瞭研究數據,積纍瞭研究資料.
목적 이용RNA-seq기술대세립극구유원두절적전록조진행측서병대측서수거진행생물신식학분석,이게시세립극구유원두절mRNA소포함적신식. 방법 용Trizol법제취원두절총RNA,분리mRNA,이용Illumina공사적HiSeq2000고통량측서의대mRNA서렬진행측서.삼고영국상격연구원(Wellcome Trust Sanger Institute)공포적세립극구조충기인조수거,대측서수거진행병접조장,장획득적unigene여비용여적단백서렬수거고(non-redundant,Nr)、전구단백자원수거고(universal protein,UniProt)、기인본체수거고(gene ontology,GO)이급일본경도기인화기인조백과전서통로수거고(the kyotoencyclopedia of genes and genomes pathway,KEGG pathway)진행비대,획득해unigene적단백공능주석신식. 결과 측서후공획득132 007 609조독단(reads),경과조장공형성91 342조unigene,평균장도위419 bp.통과GO분류주석,공유36 552개unigene획득료주석신식,분별귀입도료생물학과정、분자공능、세포조분3대류공계48개공능조.통과KEGG pathway적주석,유6 664조unigene주석도료KEGG상,삼여료6대류생물과정중34개소류적대사,공유227개대사통로. 결론 획득료세립극구유원두절전록조상관신식,위극구유병적후속연구제공료연구수거,적루료연구자료.
Objective To reveal the transcriptomic information and biological characteristics of Echinococcus granulosus protoscolex by using RNA-Seq technique and through bioinformatic analysis of sequencing data.Methods Total RNA was isolated from Echinococcus granulosus protoscolex using TRIzol.mRNA kit with Oligo(dT) magnetic beads was used to isolate poly(A) mRNA.Illumina HiSeq 2000 was applied for sequencing.Data from sequeced were assembled into unigene by using mapping-first approaches based on the genome which uploaded in the Wellcome Trust Sanger Institute.Alignment between unigenes and protein databases,such as NCBI non-redundant protein (Nr) database,UniProt database,the gene ontology (GO) database,the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database,was performed.Results A total of 132 007 609 clean reads and 91 342 unigenes were generated.The average length of unigene was 419 bp.Through analysed with GO,26 552 unigenes were mapped.Further biological process categories of GO prominently represented in biological process,molecular functions and cellular components including 48 categories.Six thousand six hundred and sixty-four unigenes were mapped to 227 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways,including 6 first level categories and 34 second level categories.Conclusion Extensive transcriptome data from sequencing of Echinococcus granulosus protoscolex were obtained.The information has provided a database for further study on the hydatid disease.
