目的 探讨Th1和Th2类细胞因子对小鼠感染血吸虫的免疫保护作用. 方法 C57BL/6小鼠48只,抽签法随机均分为4组,白细胞介素(interleukin,IL)-4组,给予IL-4 (200 ng); IL-12组,给予IL-12 (200 ng);胸腺基质淋巴生成素(thymic stromal lymphopoietin,TSLP)组,给予TSLP(200 ng);生理盐水组,给予生理盐水(200μl).4组均为腹腔注射,每周3次,持续注射8周.给药后2周,各组小鼠经皮攻击感染日本血吸虫尾蚴(40±2)条/鼠,感染后第3、6周剖杀,计算各组小鼠虫荷数、肝脏卵荷数.芯片检测各组小鼠感染前和感染后不同时间点血清中IL-5、IL-10、γ-干扰素(interferon-γ,IFN-γ)及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的动态变化.观察小鼠肝、脾的病理变化. 结果 细胞因子注射的第8周,IL-4组的Th2类细因子IL-5、IL-10依次为161.97、65.47 μg/ml; TSLP组的IL-5、IL-10依次为132.72、77.18 μg/ml; IL-12组的IL-5、IL-10依次为87.15、12.29 μg/ml;生理盐水组的IL-5、IL-10依次为188.92、167.52 μg/ml.IL-4和TSLP组Th2类细胞因子水平均高于IL-12组,但低于生理盐水组.IL-12组IFN-γ、TNF-α依次为165.7、23.64 μg/ml,水平高于其它各组.感染后3周IL-4、IL-12、TSLP、生理盐水组虫荷数依次为(9.50±4.51)、(12.83±2.32)、(6.75±2.87)、(9.80±3.03)条;感染后6周上述各组虫荷数依次为(18.83±5.91)、(24.80±9.42)、(21.67±3.67)、(17.67±6.74)条,每克肝组织虫卵数依次为(58 286.98±25 351.60)、(80 460.18±35 542.66)、(54 579.56±16 399.21)、(41 094.92±25 598.27).与生理盐水组相比,各实验组虫荷数与每克肝组织虫卵数差异均无统计学意义.感染后第3周,IL-4、IL-12、TSLP、生理盐水组脾指数依次为(0.010±0.002)、(0.011±0.002)、(0.009±0.001)、(0.007 ±0.001),各实验组与生理盐水组相比,差异均有统计学意义(t=3.158、5.076、6.204,P＜0.05).感染后第6周,IL-4、IL-12、TSLP、生理盐水组肉芽肿面积依次为(125 023.51±44403.62)、(83 238.18±27 088.95)、(113 661.30±46049.24)、(146 769.60±50 096.53) μm2.IL-12组、TSLP注射组与生理盐水注射组相比,差异均具有统计学意义(t=6.483,2.283,P＜0.05),其中以IL-12组最为显著. 结论 Th1或Th2类细胞因子单独注射对减虫或减卵没有显著作用,但对于肝脏病理变化均有一定的保护作用,并以Th1类细胞因子作用最为显著.
目的 探討Th1和Th2類細胞因子對小鼠感染血吸蟲的免疫保護作用. 方法 C57BL/6小鼠48隻,抽籤法隨機均分為4組,白細胞介素(interleukin,IL)-4組,給予IL-4 (200 ng); IL-12組,給予IL-12 (200 ng);胸腺基質淋巴生成素(thymic stromal lymphopoietin,TSLP)組,給予TSLP(200 ng);生理鹽水組,給予生理鹽水(200μl).4組均為腹腔註射,每週3次,持續註射8週.給藥後2週,各組小鼠經皮攻擊感染日本血吸蟲尾蚴(40±2)條/鼠,感染後第3、6週剖殺,計算各組小鼠蟲荷數、肝髒卵荷數.芯片檢測各組小鼠感染前和感染後不同時間點血清中IL-5、IL-10、γ-榦擾素(interferon-γ,IFN-γ)及腫瘤壞死因子-α(tumor necrosis factor-α,TNF-α)的動態變化.觀察小鼠肝、脾的病理變化. 結果 細胞因子註射的第8週,IL-4組的Th2類細因子IL-5、IL-10依次為161.97、65.47 μg/ml; TSLP組的IL-5、IL-10依次為132.72、77.18 μg/ml; IL-12組的IL-5、IL-10依次為87.15、12.29 μg/ml;生理鹽水組的IL-5、IL-10依次為188.92、167.52 μg/ml.IL-4和TSLP組Th2類細胞因子水平均高于IL-12組,但低于生理鹽水組.IL-12組IFN-γ、TNF-α依次為165.7、23.64 μg/ml,水平高于其它各組.感染後3週IL-4、IL-12、TSLP、生理鹽水組蟲荷數依次為(9.50±4.51)、(12.83±2.32)、(6.75±2.87)、(9.80±3.03)條;感染後6週上述各組蟲荷數依次為(18.83±5.91)、(24.80±9.42)、(21.67±3.67)、(17.67±6.74)條,每剋肝組織蟲卵數依次為(58 286.98±25 351.60)、(80 460.18±35 542.66)、(54 579.56±16 399.21)、(41 094.92±25 598.27).與生理鹽水組相比,各實驗組蟲荷數與每剋肝組織蟲卵數差異均無統計學意義.感染後第3週,IL-4、IL-12、TSLP、生理鹽水組脾指數依次為(0.010±0.002)、(0.011±0.002)、(0.009±0.001)、(0.007 ±0.001),各實驗組與生理鹽水組相比,差異均有統計學意義(t=3.158、5.076、6.204,P＜0.05).感染後第6週,IL-4、IL-12、TSLP、生理鹽水組肉芽腫麵積依次為(125 023.51±44403.62)、(83 238.18±27 088.95)、(113 661.30±46049.24)、(146 769.60±50 096.53) μm2.IL-12組、TSLP註射組與生理鹽水註射組相比,差異均具有統計學意義(t=6.483,2.283,P＜0.05),其中以IL-12組最為顯著. 結論 Th1或Th2類細胞因子單獨註射對減蟲或減卵沒有顯著作用,但對于肝髒病理變化均有一定的保護作用,併以Th1類細胞因子作用最為顯著.
목적 탐토Th1화Th2류세포인자대소서감염혈흡충적면역보호작용. 방법 C57BL/6소서48지,추첨법수궤균분위4조,백세포개소(interleukin,IL)-4조,급여IL-4 (200 ng); IL-12조,급여IL-12 (200 ng);흉선기질림파생성소(thymic stromal lymphopoietin,TSLP)조,급여TSLP(200 ng);생리염수조,급여생리염수(200μl).4조균위복강주사,매주3차,지속주사8주.급약후2주,각조소서경피공격감염일본혈흡충미유(40±2)조/서,감염후제3、6주부살,계산각조소서충하수、간장란하수.심편검측각조소서감염전화감염후불동시간점혈청중IL-5、IL-10、γ-간우소(interferon-γ,IFN-γ)급종류배사인자-α(tumor necrosis factor-α,TNF-α)적동태변화.관찰소서간、비적병리변화. 결과 세포인자주사적제8주,IL-4조적Th2류세인자IL-5、IL-10의차위161.97、65.47 μg/ml; TSLP조적IL-5、IL-10의차위132.72、77.18 μg/ml; IL-12조적IL-5、IL-10의차위87.15、12.29 μg/ml;생리염수조적IL-5、IL-10의차위188.92、167.52 μg/ml.IL-4화TSLP조Th2류세포인자수평균고우IL-12조,단저우생리염수조.IL-12조IFN-γ、TNF-α의차위165.7、23.64 μg/ml,수평고우기타각조.감염후3주IL-4、IL-12、TSLP、생리염수조충하수의차위(9.50±4.51)、(12.83±2.32)、(6.75±2.87)、(9.80±3.03)조;감염후6주상술각조충하수의차위(18.83±5.91)、(24.80±9.42)、(21.67±3.67)、(17.67±6.74)조,매극간조직충란수의차위(58 286.98±25 351.60)、(80 460.18±35 542.66)、(54 579.56±16 399.21)、(41 094.92±25 598.27).여생리염수조상비,각실험조충하수여매극간조직충란수차이균무통계학의의.감염후제3주,IL-4、IL-12、TSLP、생리염수조비지수의차위(0.010±0.002)、(0.011±0.002)、(0.009±0.001)、(0.007 ±0.001),각실험조여생리염수조상비,차이균유통계학의의(t=3.158、5.076、6.204,P＜0.05).감염후제6주,IL-4、IL-12、TSLP、생리염수조육아종면적의차위(125 023.51±44403.62)、(83 238.18±27 088.95)、(113 661.30±46049.24)、(146 769.60±50 096.53) μm2.IL-12조、TSLP주사조여생리염수주사조상비,차이균구유통계학의의(t=6.483,2.283,P＜0.05),기중이IL-12조최위현저. 결론 Th1혹Th2류세포인자단독주사대감충혹감란몰유현저작용,단대우간장병리변화균유일정적보호작용,병이Th1류세포인자작용최위현저.
Objective To explore the immunologically protective effects of Th1 and Th2 cytokines in mice against Schistosoma japonicum infection.Methods 48 C57BL/6 mice were divided into four groups randomly,including interleukin (IL)-4,IL-12,thymic stromal lymphopoietin (TSLP) and saline group.In each group,each mouse was given cytokine intraperitoneally three times per week for 8 weeks continuously.The dosages were IL-4 group 200 ng each mouse,IL-12 group 200 ng each mouse,TSLP group 200 ng each mouse,and normal saline group 200 μl each mouse.Two weeks after administration,each mouse was infected with 40±2 cercariae percutaneously.Three and six weeks after infection,mice were sacrificed,and the number of worms and eggs in liver were calculated.Dynamic changes of IL-5,IL-10,interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in serum of mice in different groups were detected by suspension array technology.Pathological changes of live and spleen in the mice were detected.Results Eight weeks after intraperitoneal injections of cytokines,levels of Th2 cytokines IL-5 and IL-10 in mice of IL-4 group were 161.97 and 65.47 μg/ml,which of TSLP group were 132.72 and 77.18 μg/ml,which of IL-12 group were 87.15 and 12.29 μg/ml,which of saline group were 188.92 and 167.52 μg/ml.Levels of Th2 cytokines in mice of IL-4 and TSLP groups were higher than that of IL-12 group,but lower than that of normal saline group.Levels of IFN-γ and TNF-α in the mice of IL-12 group were 165.7 and 23.64 μg/ml,which were higher than that of other groups.The worm burden of IL-4,IL-12,TSLP and saline group were(9.50±4.51),(12.83±2.32),(6.75±2.87),(9.80±3.03)respectively three weeks after infection.The worm burdens of each group were (18.83±5.91),(24.80±9.42),(21.67±3.67),(17.67±6.74),and eggs per gram of liver were(58 286.98±25 351.60),(80 460.18±35 542.66),(54 579.56±16 399.21),(41 094.92±25 598.27) respectively six weeks after infection.There were no significant differences among four groups on worm and egg burden in mice at three and six weeks after infection with Schistosomajaponicum.Three weeks after infection,splenic indexes of each group were (0.010±0.002),(0.011±0.002),(0.009±0.001) and (0.007±0.001).Compared with saline group,splenic indexes of cytokine injection groups were significantly higher than that of normal saline group (t=3.158,5.076,6.204,P＜0.05).Six weeks after infection,liver granuloma areas in the mice of cytokine injection groups were (125 023.51± 44 403.62),(83 238.18±27 088.95),(113 661.30±46 049.24) and (146 769.60±50 096.53) μm2.Liver granuloma areas in the mice of IL-12 and TSLP groups were significantly less than that of normal saline group (t=6.483,2.283,P＜0.05),and IL-12 group was the smallest.Conclusion Th1 or Th2 cytokines had no sig nificant effect on worm and egg reduction rates,but had a protective effect on pathological changes in the liver.The protective effect of Th1 cytokine was the most significant.
