国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2014年
5期
268-272
,共5页
夏厚才%罗小兵%乔铁%蔡洪英%陈根胜%周月玲
夏厚纔%囉小兵%喬鐵%蔡洪英%陳根勝%週月玲
하후재%라소병%교철%채홍영%진근성%주월령
华支睾吸虫病%胆汁%血清%灵敏度%特异性%阳性预测值
華支睪吸蟲病%膽汁%血清%靈敏度%特異性%暘性預測值
화지고흡충병%담즙%혈청%령민도%특이성%양성예측치
Clonorchiasis%Bile%Serum%Sensitivity%Specificity%Positive predictive value
目的 比较并评价粪便改良加藤厚涂片法(Kato-Katz法)、胆汁离心镜检法、血清胶体金免疫层析法(colloid gold labeled immunochromatographic,CGIA)和酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测粪便、胆汁、血清诊断华支睾吸虫感染的价值. 方法 收集92例胆结石患者的胆汁、血清和粪便,分别采用镜检法检查胆汁华支睾吸虫卵、用Kato-Katz法检查粪便华支睾吸虫卵,用CGIA法和ELISA法检测血清华支睾吸虫IgG抗体,计算并分析各检测方法的阳性率、灵敏度、特异性、阳性预测值、阴性预测值和Youden指数. 结果 Kato-Katz法联合胆汁离心镜检法共确诊感染49例,阳性率为53.3％.与之相比,Kato-Katz法、胆汁离心镜检法、CGIA和ELISA的符合率分别为80.4％、84.8％、90.2％和96.7％.Kato-Katz法、胆汁离心镜检法、CGIA和ELISA法的灵敏度分别是63.3％、93.9％、98.0％和93.9％;特异性分别为100％、100％、69.8％和86.0％;阳性预测值则分别是100％、100％、78.7％和88.5％,阴性预测值分别为70.5％、93.5％、96.8％和92.5％,Youden指数为0.63、0.94、0.68和0.80. 结论 胆汁离心镜检是胆结石手术患者诊断华支睾吸虫感染的首选方法;但对于无法获得胆汁的受检者而言,血清IgG抗体检测在排除华支睾吸虫感染、粪便Kato-Katz法在确诊感染方面有较好的临床应用价值.
目的 比較併評價糞便改良加籐厚塗片法(Kato-Katz法)、膽汁離心鏡檢法、血清膠體金免疫層析法(colloid gold labeled immunochromatographic,CGIA)和酶聯免疫吸附試驗(enzyme-linked immunosorbent assay,ELISA)檢測糞便、膽汁、血清診斷華支睪吸蟲感染的價值. 方法 收集92例膽結石患者的膽汁、血清和糞便,分彆採用鏡檢法檢查膽汁華支睪吸蟲卵、用Kato-Katz法檢查糞便華支睪吸蟲卵,用CGIA法和ELISA法檢測血清華支睪吸蟲IgG抗體,計算併分析各檢測方法的暘性率、靈敏度、特異性、暘性預測值、陰性預測值和Youden指數. 結果 Kato-Katz法聯閤膽汁離心鏡檢法共確診感染49例,暘性率為53.3％.與之相比,Kato-Katz法、膽汁離心鏡檢法、CGIA和ELISA的符閤率分彆為80.4％、84.8％、90.2％和96.7％.Kato-Katz法、膽汁離心鏡檢法、CGIA和ELISA法的靈敏度分彆是63.3％、93.9％、98.0％和93.9％;特異性分彆為100％、100％、69.8％和86.0％;暘性預測值則分彆是100％、100％、78.7％和88.5％,陰性預測值分彆為70.5％、93.5％、96.8％和92.5％,Youden指數為0.63、0.94、0.68和0.80. 結論 膽汁離心鏡檢是膽結石手術患者診斷華支睪吸蟲感染的首選方法;但對于無法穫得膽汁的受檢者而言,血清IgG抗體檢測在排除華支睪吸蟲感染、糞便Kato-Katz法在確診感染方麵有較好的臨床應用價值.
목적 비교병평개분편개량가등후도편법(Kato-Katz법)、담즙리심경검법、혈청효체금면역층석법(colloid gold labeled immunochromatographic,CGIA)화매련면역흡부시험(enzyme-linked immunosorbent assay,ELISA)검측분편、담즙、혈청진단화지고흡충감염적개치. 방법 수집92례담결석환자적담즙、혈청화분편,분별채용경검법검사담즙화지고흡충란、용Kato-Katz법검사분편화지고흡충란,용CGIA법화ELISA법검측혈청화지고흡충IgG항체,계산병분석각검측방법적양성솔、령민도、특이성、양성예측치、음성예측치화Youden지수. 결과 Kato-Katz법연합담즙리심경검법공학진감염49례,양성솔위53.3％.여지상비,Kato-Katz법、담즙리심경검법、CGIA화ELISA적부합솔분별위80.4％、84.8％、90.2％화96.7％.Kato-Katz법、담즙리심경검법、CGIA화ELISA법적령민도분별시63.3％、93.9％、98.0％화93.9％;특이성분별위100％、100％、69.8％화86.0％;양성예측치칙분별시100％、100％、78.7％화88.5％,음성예측치분별위70.5％、93.5％、96.8％화92.5％,Youden지수위0.63、0.94、0.68화0.80. 결론 담즙리심경검시담결석수술환자진단화지고흡충감염적수선방법;단대우무법획득담즙적수검자이언,혈청IgG항체검측재배제화지고흡충감염、분편Kato-Katz법재학진감염방면유교호적림상응용개치.
Objective To compare and evaluate the value of Kato-Katz method,bile precipitate microscopic examination,colloid gold labeled immunochromatographic (CGIA) and enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Clonorchis sinensis (C.sinensis) infection.Methods The feces,bile and serum specimens from 92 patients with gallstone were collected.The feces and bile were examined under microscopy for C.sinensis eggs using Kato-Katz method and bile precipitation respectively.Meanwhile,the serum IgG of C.sinensis was detected with CGIA and ELISA.Finally,the detection rate,sensitivity,specificity,positive predictive value(PPV),negative predictive value (NPV) and Youden index (YI) were analyzed and compared.Results Of the 92 patients,49 cases were diagnosed with C.sinensis infection using Kato-Katz method combined with bile precipitation microscopic examination,and the infection rate was 53.3％.Comparing to this,the coincidence rates of Kato-Katz method,bile precipitation microscopic examination,CGIA,and ELISA were 80.4％,84.8％,90.2％ and 96.7％,respectively.Meanwhile,the diagnostic values of Kato-Katz method,bile precipitation microscopic examination,CGIA and ELISA were 63.3％,93.9％,98.0％ and 93.9％ in sensitivity;100％,100％,69.8％ and 86.0％ in specificity; 70.5％,93.5％,96.8％ and 92.5％ in NPV,100％,100％,78.7％ and 88.5％ in PPV and 0.63,0.94,0.68 and 0.80 in YI,respectively.Conclusion Bile precipitation microscopic examination is the most preferred diagnostic method of C.sinensis infection for the gallstone patients subject ed surgery.Whereas,for those subjects who can not provide bile,serum IgG antibody detection to exclude C.sinensis infection and feces Kato-Katz method to confirm infection have good clinical application value.
