CAJ | 학술논문

目的通过对杭州市萧山区1例疑似人感染禽流感病人咽拭子和鼻腔冲洗液样本进行检测和分析,加深对A型流感病毒抗原变异特性的认识,进一步提高实验室相应的检测能力.方法以实时荧光定量PCR(real-time PCR)检测流感病毒核酸,对未能分型的HA、NA和M基因通过逆转录PCR(RT-PCR)扩增而后克隆测定;同时接种狗肾细胞(MDCK)进行病毒培养.结果样本A型流感病毒核酸检测为阳性,但未能分型;HA、NA、M基因测序结果表明与禽源H7、N9、M基因相似性最高;同时A型流感病毒培养也呈阳性.结论经过浙江省CDC与中国CDC证实,该患者感染H7N9禽流感病毒,为浙江省首个病例.
목적 통과대항주시소산구1례의사인감염금류감병인인식자화비강충세액양본진행검측화분석,가심대A형류감병독항원변이특성적인식,진일보제고실험실상응적검측능력.방법 이실시형광정량PCR(real-time PCR)검측류감병독핵산,대미능분형적HA、NA화M기인통과역전록PCR(RT-PCR)확증이후극륭측정;동시접충구신세포(MDCK)진행병독배양.결과 양본A형류감병독핵산검측위양성,단미능분형;HA、NA、M기인측서결과표명여금원H7、N9、M기인상사성최고;동시A형류감병독배양야정양성.결론 경과절강성CDC여중국CDC증실,해환자감염H7N9금류감병독,위절강성수개병례.
Objective To detect and analyze the throat swab and nasal cavity lavaging fluid samples from one suspected human avian influenza in Xiaoshan,in order to deepen the understanding of the antigenic variation characteristics of influenza A virus,and to strengthen laboratory diagnostic ability.Methods The influenza virus nucleic acid from the samples were detected by real-time fluorescent quantitative PCR (real-time PCR).In addition,unallocated HA,NA and M genes were amplified by reverse transcription PCR (RT-PCR) and sequenced after cloning.Meanwhile,MDCK cells were inoculated with the sample for viral culturing.Results RNA extracted from the sample was positive for influenza A virus nucleic acid,but could not be typed.Furthermore,the results of gene sequencing revealed that the HA,NA,M genes were most closely related to H7,N9,M genes of avian origin,respectively,and the viral culturing was also positive for influenza A virus.Conclusions The patient was infected with a novel avian-origin influenza A (H7N9) virus,and was the first case in Zhejiang Province.