CAJ | 학술논문

目的研究电焊工血液中重金属含量及其与DNA损伤之间的关系.方法抽取22名来自医疗器械制造企业(A组)、25名来自船舶制造企业(B组)的电焊工以及22名管理人员(对照组)的外周静脉血,用电感耦合等离子体质谱仪(ICP-MS)检测血液中铬(Cr)、锌(Zn)、镉(Cd)、铅(Pb)、钴(Co)、镍(Ni)、锰(Mn)的含量,并用彗星试验检测外周血DNA损伤.用多元线性回归法及相关性分析法分析重金属含量与DNA损伤之间的关系.结果与对照组相比,A,B组电焊工血液中Pb和Mn含量均明显增高,差异有统计学意义(A组:UPb=136.0,tMn=-6.2;B组:UPb=78.5,tMn=-7.6,P＜0.01),A组电焊工血液中Cr、Ni含量较高,差异有统计学意义(Ucr=64.0,tNi =-4.4,P＜0.01);B组电焊工血液中Cd含量较高,差异有统计学意义(U=260.0,P＜0.01).多元线性回归分析显示,控制B组与对照组饮酒史的差异因素后,B组电焊工外周血DNA损伤仍显著高于对照(β=3.9,SE=0.9,t =4.3,P＜0.01).但相关性分析显示,两组电焊工血液中各重金属含量与外周血DNA损伤均无显著相关性(P＞0.05).结论电焊工血液中的重金属负荷种类可能与电焊丝的材料有关,但本研究并未发现单一重金属负荷与外周血DNA损伤存在相关性.B企业外周血DNA损伤可能与其他未明因素有关.
목적 연구전한공혈액중중금속함량급기여DNA손상지간적관계.방법 추취22명래자의료기계제조기업(A조)、25명래자선박제조기업(B조)적전한공이급22명관리인원(대조조)적외주정맥혈,용전감우합등리자체질보의(ICP-MS)검측혈액중락(Cr)、자(Zn)、력(Cd)、연(Pb)、고(Co)、얼(Ni)、맹(Mn)적함량,병용혜성시험검측외주혈DNA손상.용다원선성회귀법급상관성분석법분석중금속함량여DNA손상지간적관계.결과 여대조조상비,A,B조전한공혈액중Pb화Mn함량균명현증고,차이유통계학의의(A조:UPb=136.0,tMn=-6.2;B조:UPb=78.5,tMn=-7.6,P＜0.01),A조전한공혈액중Cr、Ni함량교고,차이유통계학의의(Ucr=64.0,tNi =-4.4,P＜0.01);B조전한공혈액중Cd함량교고,차이유통계학의의(U=260.0,P＜0.01).다원선성회귀분석현시,공제B조여대조조음주사적차이인소후,B조전한공외주혈DNA손상잉현저고우대조(β=3.9,SE=0.9,t =4.3,P＜0.01).단상관성분석현시,량조전한공혈액중각중금속함량여외주혈DNA손상균무현저상관성(P＞0.05).결론 전한공혈액중적중금속부하충류가능여전한사적재료유관,단본연구병미발현단일중금속부하여외주혈DNA손상존재상관성.B기업외주혈DNA손상가능여기타미명인소유관.
Objective To investigate the concentrations of heavy metals in peripheral blood from welders and its relationship with blood DNA damage.Methods The blood samples were collected from 22 welders of the medical equipment manufacture enterprise (group A),25 welders of shipbuilding enterprise (group B) and 22 administrators (control group).The concentrations of chromium (Cr),zinc (Zn),cadmium (Cd),lead (Pb),cobalt (Co),nickel (Ni),and manganese (Mn) in blood were detected by inductively coupled plasma-mass spectrometry(ICP-MS).The DNA damage of peripheral blood was measured by comet assay.The relationship between metal concentrations and the levels of DNA damage was analyzed by multiple linear regression and correlation analysis.Results Compared with control group,blood concentrations of Pb and Mn were higher in group A and B (group A:UPb =136.0,tMn=-6.2; group B:UPb =78.5,tMn=-7.6; P all ＜ 0.01),blood levels of Cr and Ni were significantly higher in group A(UCr =64.0,tNi =-4.4; P ＜0.01)and blood concentrations of Cd were higher in group B respectively(UCd =260.0,P ＜ 0.01).The results of multiple linear regression analysis indicated that the level of DNA damage in B group were significantly higher than the control group after the control of drinking factor (β =3.9,SE =0.9,t =4.3,P ＜ 0.01).However the results of correlation analysis indicated that there was no correlation between blood concentrations of heavy metals and the levels of DNA damage (P ＞ 0.05).Conclusions Differences of heavy metal burdens in blood from welders may be related to the steel wires used in welding.But no correlation between DNA damage and any single metal burden in blood is found in this study.The blood DNA damage observed in group B may attribute to other unclear factors.