国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2013年
4期
236-238,封3
,共4页
吴凌康%厉有名%刘英超%施维群%吴峰%茹清静
吳凌康%厲有名%劉英超%施維群%吳峰%茹清靜
오릉강%려유명%류영초%시유군%오봉%여청정
肝星形细胞%GLP-1受体%免疫印迹%RT-PCR%抗肝纤维化
肝星形細胞%GLP-1受體%免疫印跡%RT-PCR%抗肝纖維化
간성형세포%GLP-1수체%면역인적%RT-PCR%항간섬유화
Hpatic stellate cell%GLP-1 receptor%Western bloting%RT-PCR%Anti-hepatic fibrosis
目的 证实胰高血糖素样肽1(GLP-1)受体在人肝星形细胞上的表达.方法 体外培养人肝星形细胞并进行形态学鉴定.提取肝星形细胞mRNA并逆转录获得cDNA,RT-PCR法测定GLP-1受体mR-NA的表达,免疫印迹法测定GLP-1受体蛋白的表达情况.结果 显微镜下可以观察到活化的肝星形细胞为扁平状,胞体大,具有发育良好的应力纤维,胞浆内缺乏脂肪滴.GLP-1受体cDNA片段长度为296 bp,与GenBank公布的人GLP-1受体cDNA序列(NM-002062.3)相符度为100％.免疫印迹法表明人肝星形细胞样本GLP-1受体蛋白表达阳性.结论 人肝星形细胞上存在GLP-1受体的表达.
目的 證實胰高血糖素樣肽1(GLP-1)受體在人肝星形細胞上的錶達.方法 體外培養人肝星形細胞併進行形態學鑒定.提取肝星形細胞mRNA併逆轉錄穫得cDNA,RT-PCR法測定GLP-1受體mR-NA的錶達,免疫印跡法測定GLP-1受體蛋白的錶達情況.結果 顯微鏡下可以觀察到活化的肝星形細胞為扁平狀,胞體大,具有髮育良好的應力纖維,胞漿內缺乏脂肪滴.GLP-1受體cDNA片段長度為296 bp,與GenBank公佈的人GLP-1受體cDNA序列(NM-002062.3)相符度為100％.免疫印跡法錶明人肝星形細胞樣本GLP-1受體蛋白錶達暘性.結論 人肝星形細胞上存在GLP-1受體的錶達.
목적 증실이고혈당소양태1(GLP-1)수체재인간성형세포상적표체.방법 체외배양인간성형세포병진행형태학감정.제취간성형세포mRNA병역전록획득cDNA,RT-PCR법측정GLP-1수체mR-NA적표체,면역인적법측정GLP-1수체단백적표체정황.결과 현미경하가이관찰도활화적간성형세포위편평상,포체대,구유발육량호적응력섬유,포장내결핍지방적.GLP-1수체cDNA편단장도위296 bp,여GenBank공포적인GLP-1수체cDNA서렬(NM-002062.3)상부도위100％.면역인적법표명인간성형세포양본GLP-1수체단백표체양성.결론 인간성형세포상존재GLP-1수체적표체.
Objective To confirm the expression of glucagon-like peptide 1 (GLP-1) receptor in human hepatic stellate cell (HSC).Methods Human HSC was cultured in vitro and morphologically identified.The mRNA of HSC was extracted and reversely transcribed to obtain cDNA.The mRNA of GLP-1 receptor was determined by RT-PCR.The GLP-1 receptor protein was determined by western blotting.Results The activating HSC were flat,with large cell body and well-developed stress fibers and without fat droplets in the cytoplasm under the microscope.The length of GLP-1 receptor cDNA fragment was 296 bp,which was 100％ consistent with the human GLP-1 receptor eDNA sequence (NM-002062.3) published in Gen Bank.The results of western blotting showed that there was GLP-1 receptor protein expressed in human HSC.Conclusions The GLP-1 receptor express in human HSC.
