CAJ | 학술논문

目的分析宁波市2009-2011年水痘-带状疱疹病毒(VZV)的流行病学及流行株基因分型特征.方法收集宁波市突发公共卫生事件报告管理系统的水痘病例发病资料,采集部分水痘病例疱疹液样本,采用PCR法对VZV流行株病毒进行基因分型及限制性内切酶SmaⅠ进行流行株鉴定,用MRC-5细胞进行VZV的分离.结果 2009-2011年宁波市水痘病例的报告发病率分别为23.33/10万、26.24/10万和36.25/10万.7～ 12岁儿童发病率最高,达245.21/10万；5-6月份和11-12月份分别为两个季节性发病高峰.32份水痘患者水疱液样本中25份检出VZV核酸阳性样本,基因分型结果提示为2种不同基因型的毒株,其中2份为M1基因型,其余23份为J基因型毒株.结论宁波市水痘病例主要发生在春末夏初和秋末冬初季节,以7～12岁学龄儿童为主,J基因型毒株目前为本市流行主要毒株,但也有M1基因型毒株的局部流行.
목적 분석저파시2009-2011년수두-대상포진병독(VZV)적류행병학급류행주기인분형특정.방법 수집저파시돌발공공위생사건보고관리계통적수두병례발병자료,채집부분수두병례포진액양본,채용PCR법대VZV류행주병독진행기인분형급한제성내절매SmaⅠ진행류행주감정,용MRC-5세포진행VZV적분리.결과 2009-2011년저파시수두병례적보고발병솔분별위23.33/10만、26.24/10만화36.25/10만.7～ 12세인동발병솔최고,체245.21/10만；5-6월빈화11-12월빈분별위량개계절성발병고봉.32빈수두환자수포액양본중25빈검출VZV핵산양성양본,기인분형결과제시위2충불동기인형적독주,기중2빈위M1기인형,기여23빈위J기인형독주.결론 저파시수두병례주요발생재춘말하초화추말동초계절,이7～12세학령인동위주,J기인형독주목전위본시류행주요독주,단야유M1기인형독주적국부류행.
Objective To analyze the varicella-zoster virus (VZV) epidemiology and the genotype characteristics of epidemic strains in Ningbo from 2009 to 2011.Methods The incidence data of chickenpox cases from Ningbo public health emergency report management system were collected and analyzed.Some vesicle fluid samples from chickenpox cases were collected.The VZV epidemic strains were genotyped by PCR method and the epidemic strains were identified by the restriction enyme Sma Ⅰ.The VZV virus was isolated by MRC-5 cells.Results The reported incidence rates of chickenpox in Ningbo were 23.33/100 000,26.24/100 000 and 36.25/100 000 from 2009 to 2011.Children aged 7-12 years had the highest incidence rate (245.21/100 000).Two seasonal peaks of chickenpox incidence were from May to June and November to December,respectively.The PCR detection result of VZV from 32 vesicle fluid samples showed that 25 samples were positive.Genotype results showed 2 kinds of different genetic types of strain:2 samples were M1 type strains,and the other 23 samples were J type strains.Conclusions Ningbo chickenpox cases mainly occur in late spring to early summer and late autumn to early winter,with the school-age children aged 7-12 years as the main patients.J strains are the major epidemic swain,but also have the local popular Ml type strain.