CAJ | 학술논문

目的探讨间接免疫荧光法(IFA)对急性发热伴血小板减少综合征布尼亚病毒(SFTSV)感染的诊断价值,并了解宁波市象山县SFTSV流行区健康人群的抗体水平.方法非洲绿猴肾细胞(Vero细胞)分离的本地SFTSV株制作抗原片,以IFA法和ELISA法对象山县265份健康人群血清进行SFTSV抗体测定,并用病毒微量中和试验(MNT)验证两种方法的检测效果.结果 265份象山县健康人群血清中,IgG抗体经IFA法检测共36份阳性,阳性率为13.58％ (36/265);经ELISA法检测,共23份阳性,阳性率为8.68％ (23/265);两者差异有统计学意义(x2=9.624,P＜0.05).与MNT法检出结果对比,IFA法诊断的符合率为89.19(33/37),而ELISA法的符合率为70.27％ (26/37);两者比较差异有统计学意义(x2=4.097,P＜0.05).所有SFTSV-IgG阳性样本中SFTSV-IgM同时阳性共23份,占62.16％ (23/37).结论 IFA法可同时检测SFTSV-IgG和-IgM抗体,可作为未在病毒血症期内就症病例的补充诊断方法,也适合对人群抗体水平的调查.宁波市象山县健康人群中检出较高水平的SFTSV-IgG抗体,表明存在SFTSV的流行以及隐性感染;SFTSV-IgM阳性提示这些感染为新近感染.
목적 탐토간접면역형광법(IFA)대급성발열반혈소판감소종합정포니아병독(SFTSV)감염적진단개치,병료해저파시상산현SFTSV류행구건강인군적항체수평.방법 비주록후신세포(Vero세포)분리적본지SFTSV주제작항원편,이IFA법화ELISA법대상산현265빈건강인군혈청진행SFTSV항체측정,병용병독미량중화시험(MNT)험증량충방법적검측효과.결과 265빈상산현건강인군혈청중,IgG항체경IFA법검측공36빈양성,양성솔위13.58％ (36/265);경ELISA법검측,공23빈양성,양성솔위8.68％ (23/265);량자차이유통계학의의(x2=9.624,P＜0.05).여MNT법검출결과대비,IFA법진단적부합솔위89.19(33/37),이ELISA법적부합솔위70.27％ (26/37);량자비교차이유통계학의의(x2=4.097,P＜0.05).소유SFTSV-IgG양성양본중SFTSV-IgM동시양성공23빈,점62.16％ (23/37).결론 IFA법가동시검측SFTSV-IgG화-IgM항체,가작위미재병독혈증기내취증병례적보충진단방법,야괄합대인군항체수평적조사.저파시상산현건강인군중검출교고수평적SFTSV-IgG항체,표명존재SFTSV적류행이급은성감염;SFTSV-IgM양성제시저사감염위신근감염.
Objective To explore the early diagnostic value of indirect immunofluorescence assay (IFA) in detection of severe fever with thrombocytopenia syndrome bunyavirus (SFTSV),and to have an overview on the level of antibody against SFTSV in healthy population of Ningbo Xiangshan.Methods Local SFTSV strains isolated from African green monkey kidney cells (Vero cells) were used to produce antigen films.SFTSV antibody in blood samples from 265 healthy individuals in Xiangshan were detected by IFA and ELISA.The results of IFA and ELISA were compared with that of micro-neutralization test (MNT).Results Of 265 serum samples,the positive rates of SFTSV-IgG detected by IFA and ELISA were 13.58％ (36/256) and 8.68％ (23/256),respectively,with a significant difference (x2=9.624,P＜0.05).The coincidence rate of MNT and IFA was 89.19％ (33/37) which was higher than 70.27％ (26/37) of MNT and ELISA,with a significant difference between them (x2=4.097,P＜0.05).Both of IgM and IgG were detected positive in 23 samples,accounting for 62.16％ (23/37) of IgG positive samples.Conclusions IFA can be used as a complementary method for diagnosis of SFTSV infection when viremia is absent because of its ability to detect IgM and IgG together.IFA is also suitable for antibody detection.High level of SFTSV-IgG antibody in healthy population of Ningbo Xiangshan suggests the epidemic and latent infection of SFTSV in local area.SFTSV-IgM detected positive in healthy individuals means the possibility of new infection in recent years.