国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2013年
1期
57-59
,共3页
唐小云%姬云丽%杨美荣%张晓莉%桂金秋%刘亚威%LI Fu-juan%李福娟%聂影
唐小雲%姬雲麗%楊美榮%張曉莉%桂金鞦%劉亞威%LI Fu-juan%李福娟%聶影
당소운%희운려%양미영%장효리%계금추%류아위%LI Fu-juan%리복연%섭영
妊娠特异性糖蛋白%树突状细胞%同种淋巴细胞反应
妊娠特異性糖蛋白%樹突狀細胞%同種淋巴細胞反應
임신특이성당단백%수돌상세포%동충림파세포반응
Pregnant specific glycoprotein%Dendritic cells%Allogeneic lymphocyte reaction
目的 探讨妊娠特异性糖蛋白(PSG)对Balb/c小鼠脾脏来源的树突状细胞(DC)的免疫调节作用.方法 无菌分离Balb/c小鼠脾脏单个核细胞,rmGM-CSF、rmIL-4诱导分化为DC,同时施加0.2 g/L PSG作用7d,并设对照组,流式细胞仪检测DC表面标志和抗原摄取能力,MTT法检测其同种异基因淋巴细胞刺激能力.结果 与对照组相比较,DC表面标志MHC-Ⅱ类分子、CD80、CD86和CD40的表达显著低于对照组(t=2.52,t =3.24,=2.74,t =2.58,P＜0.01),DC抗原摄取能力亦明显降低(t=3.15,P＜0.01),刺激细胞:反应细胞比例为1∶10和1∶20的PSG作用后的DC刺激同种T淋巴细胞增殖的能力明显降低(t=3.12,t =2.76,P＜0.01),比例为1∶50和1∶100的PSG组T淋巴细胞增殖能力也有降低(t=2.34,t =2.47,P＜0.05).结论 PSG可下调Balb/c小鼠脾脏DC的免疫功能,可能与妊娠免疫抑制有关.
目的 探討妊娠特異性糖蛋白(PSG)對Balb/c小鼠脾髒來源的樹突狀細胞(DC)的免疫調節作用.方法 無菌分離Balb/c小鼠脾髒單箇覈細胞,rmGM-CSF、rmIL-4誘導分化為DC,同時施加0.2 g/L PSG作用7d,併設對照組,流式細胞儀檢測DC錶麵標誌和抗原攝取能力,MTT法檢測其同種異基因淋巴細胞刺激能力.結果 與對照組相比較,DC錶麵標誌MHC-Ⅱ類分子、CD80、CD86和CD40的錶達顯著低于對照組(t=2.52,t =3.24,=2.74,t =2.58,P＜0.01),DC抗原攝取能力亦明顯降低(t=3.15,P＜0.01),刺激細胞:反應細胞比例為1∶10和1∶20的PSG作用後的DC刺激同種T淋巴細胞增殖的能力明顯降低(t=3.12,t =2.76,P＜0.01),比例為1∶50和1∶100的PSG組T淋巴細胞增殖能力也有降低(t=2.34,t =2.47,P＜0.05).結論 PSG可下調Balb/c小鼠脾髒DC的免疫功能,可能與妊娠免疫抑製有關.
목적 탐토임신특이성당단백(PSG)대Balb/c소서비장래원적수돌상세포(DC)적면역조절작용.방법 무균분리Balb/c소서비장단개핵세포,rmGM-CSF、rmIL-4유도분화위DC,동시시가0.2 g/L PSG작용7d,병설대조조,류식세포의검측DC표면표지화항원섭취능력,MTT법검측기동충이기인림파세포자격능력.결과 여대조조상비교,DC표면표지MHC-Ⅱ류분자、CD80、CD86화CD40적표체현저저우대조조(t=2.52,t =3.24,=2.74,t =2.58,P＜0.01),DC항원섭취능력역명현강저(t=3.15,P＜0.01),자격세포:반응세포비례위1∶10화1∶20적PSG작용후적DC자격동충T림파세포증식적능력명현강저(t=3.12,t =2.76,P＜0.01),비례위1∶50화1∶100적PSG조T림파세포증식능력야유강저(t=2.34,t =2.47,P＜0.05).결론 PSG가하조Balb/c소서비장DC적면역공능,가능여임신면역억제유관.
Objective To investigate the regulation of pregnant specific glycoprotein (PSG) on dendritic cells derived from spleen of Balb/c mice.Methods Spleen mononuclear cells of Balb/c isolated under sterile conditions were induced to differentiate into dendritic cells with rmGM-CSF and rmIL-4,Cells were treated with 0.2 g/L PSG for 7 days.Surface markers and the capacity of antigen uptaking of dendritic cells were analyzed with flow cytometry.The capacity of DCs in stimulating allogene lymphocytes was detected by methyl thiazolyl tetrazolium (MTT) assay.Results Compared with control,the dendritic cells treated with PSG expressed lower MHC-Ⅱ,CD80,CD86 and CD40 (t =2.52,t =3.24,t =2.74,t =2.58,P ＜0.01).The competence of antigen uptaking decreased differently (t =3.24 and P ＜ 0.01).The stimulating capability of allogeneic T lymphocytes that the ratio of stimulating cell and responding cell was 1 ∶ 10 and 1 ∶ 20 decreased apparently(t =3.12,t =2.76,P ＜0.01) and that of 1∶50 and 1∶ 100 also decreased(t =2.34,t =2.47,P ＜0.05).Conclusion PSG can down-regulate the immune function of the dendritic cells derived from spleen of Balb/c mice.This negative effective effect of PSG might be associated with pregnant immune suppression.
