国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2013年
2期
138-141
,共4页
安凤超%马直勉%李海勇%刘平%高翔
安鳳超%馬直勉%李海勇%劉平%高翔
안봉초%마직면%리해용%류평%고상
肝吸虫%细胞因子%黄芪%阿苯达唑
肝吸蟲%細胞因子%黃芪%阿苯達唑
간흡충%세포인자%황기%아분체서
Clonorchis sinensis%Cytokine%Milkvetch Root%Albendazole
目的 探讨细胞因子在肝吸虫发病机制中的作用和中药黄芪保护肝组织的机制.方法 健康成年Wistar大鼠32只随机分为对照组、模型组、阿苯达唑(ALB)治疗组和阿苯达唑联合黄芪(ALB+ MR)治疗组,建立肝吸虫病大鼠动物模型.ALB治疗剂量50 mg/kg/d/只,连用5天；MR腹腔注射800 mg/kg/d/只,连用30天.感染14周后处死大鼠,光镜观察大鼠肝组织病理改变,ELISA法检测大鼠血清细胞因子IL-2和IL-4水平.结果 对照组大鼠肝组织未见异常,模型组大鼠肝组织病理改变显著,阿苯达唑和黄芪治疗后病理改变明显减轻.与对照组比较,模型组大鼠血清IL-2水平下降(F=18.3,P=0.000 2),IL-4水平升高(F=10.44,P=0.003 4).治疗后,ALB组和ALB+ MR组大鼠血清IL-2水平升高(F=8.69,P=0.006 8；F=5.5,P=0.027 3),IL-4水平下降(F=9.69,P=0.004 6；F=7.61,P=0.010 7)阿苯达唑联合黄芪治疗效果更好.结论 细胞因子IL-2和IL-4参与肝吸虫引起的肝组织损伤,黄芪对肝组织具有保护作用,其机制可能与调节紊乱的细胞因子有关.
目的 探討細胞因子在肝吸蟲髮病機製中的作用和中藥黃芪保護肝組織的機製.方法 健康成年Wistar大鼠32隻隨機分為對照組、模型組、阿苯達唑(ALB)治療組和阿苯達唑聯閤黃芪(ALB+ MR)治療組,建立肝吸蟲病大鼠動物模型.ALB治療劑量50 mg/kg/d/隻,連用5天；MR腹腔註射800 mg/kg/d/隻,連用30天.感染14週後處死大鼠,光鏡觀察大鼠肝組織病理改變,ELISA法檢測大鼠血清細胞因子IL-2和IL-4水平.結果 對照組大鼠肝組織未見異常,模型組大鼠肝組織病理改變顯著,阿苯達唑和黃芪治療後病理改變明顯減輕.與對照組比較,模型組大鼠血清IL-2水平下降(F=18.3,P=0.000 2),IL-4水平升高(F=10.44,P=0.003 4).治療後,ALB組和ALB+ MR組大鼠血清IL-2水平升高(F=8.69,P=0.006 8；F=5.5,P=0.027 3),IL-4水平下降(F=9.69,P=0.004 6；F=7.61,P=0.010 7)阿苯達唑聯閤黃芪治療效果更好.結論 細胞因子IL-2和IL-4參與肝吸蟲引起的肝組織損傷,黃芪對肝組織具有保護作用,其機製可能與調節紊亂的細胞因子有關.
목적 탐토세포인자재간흡충발병궤제중적작용화중약황기보호간조직적궤제.방법 건강성년Wistar대서32지수궤분위대조조、모형조、아분체서(ALB)치료조화아분체서연합황기(ALB+ MR)치료조,건립간흡충병대서동물모형.ALB치료제량50 mg/kg/d/지,련용5천；MR복강주사800 mg/kg/d/지,련용30천.감염14주후처사대서,광경관찰대서간조직병리개변,ELISA법검측대서혈청세포인자IL-2화IL-4수평.결과 대조조대서간조직미견이상,모형조대서간조직병리개변현저,아분체서화황기치료후병리개변명현감경.여대조조비교,모형조대서혈청IL-2수평하강(F=18.3,P=0.000 2),IL-4수평승고(F=10.44,P=0.003 4).치료후,ALB조화ALB+ MR조대서혈청IL-2수평승고(F=8.69,P=0.006 8；F=5.5,P=0.027 3),IL-4수평하강(F=9.69,P=0.004 6；F=7.61,P=0.010 7)아분체서연합황기치료효과경호.결론 세포인자IL-2화IL-4삼여간흡충인기적간조직손상,황기대간조직구유보호작용,기궤제가능여조절문란적세포인자유관.
Objective To investigate the changes of cytokines in rats with Clonorchiasis and the protective effects of Milkvetch Root on liver tissue.Methods Adult 32 male rats were randomly divided into four groups:control group,model group,Albendazole (ALB) group and Albendazole combined with Milkvetch Root (ALB + MR) group (8 rats / group).The experimental rats were infected with 50 metacercariae of C.sinensis or equal physiological saline through a gavage needle.The ALB group and ALB + MR group received ALB (50mg/kg/d) orally for 5days,or with MR intraperitoneally (800 mg/kg/d) for 30 days.All rats were sacrificed after 14 weeks of infection.Histopathological examination of rats' livers was performed.The levels of IL-2 and IL-4 in the serum of the rats were detected by ELISA assays.Results The vacuolization of hepatocytes and infiltration of white cells were observed in the model group,but did not observed in control group under light microscope.The injury of liver tissue of rats in ALB + MR group was milder than ALB group.The level of IL-4 in serum of rats in model group increased significantly(F =10.44,P =0.003 4),but the level of IL-2 decreased significantly compared with control group (F =18.3,P =0.000 2).The level of IL-4 in serum of rats in ALB group and in A LB + MR group decreased significantly (F =9.69,P =0.0046 ; F =7.61,P =0.010 7),while the level of IL-2 of rats in ALB group and in ALB + MR group increased significantly (F =8.69,P =0.006 8 ; F =5.5,P =0.027 3).Conclusion Our results suggested that IL-2 and IL-4 were involved in the pathogenesis of clonorchis sinensis.Milkvetch Root could provide protection to the liver tissue against Clonorchis sinensis,which might associate with the regulation of disturbed cytokines.
