CAJ | 학술논문

目的探讨血清内皮素(ET)-1、一氧化氮(NO)在2型糖尿病大鼠心肌缺血再灌注损伤过程中的变化及意义.方法选择体重160 ～ 180 g的健康雄性Wistar大鼠,采用高脂饲养联合腹腔注射链脲佐菌素(STZ)的方法制备2型糖尿病模型,取造模成功后的糖尿病大鼠14只(D组),随机分为两组(n=7):糖尿病心肌缺血再灌注组(DI组)和糖尿病假手术组(DC组).年龄匹配的健康雄性Wistar大鼠14只作为对照(C组),随机分为两组(n=7):心肌缺血再灌注组(CI组)和假手术组(CC组).DI组和CI组采用结扎左冠状动脉前降支30 min再灌注120 min的方法制备心肌缺血再灌注模型.各组分别测定基础状态、再灌注120 min时血清NO、ET-1的含量.结果与基础状态时比较,CI组、DI组在缺血再灌注120 min时血清NO水平降低、ET-1水平升高(t值分别为4.96、4.69、19.04和3.35,P＜0.05).与CC组比较,CI组NO水平降低、ET-1水平升高,差异具有统计学差异(F=18.07、11.97,P＜0.05)；与DC组比较,DI组NO水平降低、ET-1水平升高具有统计学差异(F=4.15、8.04,P＜0.05).DC组基础状态时血清ET-1水平高于CC组,具有统计学差异(=9.47,P＜0.05).结论血清中NO、ET-1在2型糖尿病大鼠心肌缺血再灌注损伤的病理生理过程中具有一定的意义.
목적 탐토혈청내피소(ET)-1、일양화담(NO)재2형당뇨병대서심기결혈재관주손상과정중적변화급의의.방법 선택체중160 ～ 180 g적건강웅성Wistar대서,채용고지사양연합복강주사련뇨좌균소(STZ)적방법제비2형당뇨병모형,취조모성공후적당뇨병대서14지(D조),수궤분위량조(n=7):당뇨병심기결혈재관주조(DI조)화당뇨병가수술조(DC조).년령필배적건강웅성Wistar대서14지작위대조(C조),수궤분위량조(n=7):심기결혈재관주조(CI조)화가수술조(CC조).DI조화CI조채용결찰좌관상동맥전강지30 min재관주120 min적방법제비심기결혈재관주모형.각조분별측정기출상태、재관주120 min시혈청NO、ET-1적함량.결과 여기출상태시비교,CI조、DI조재결혈재관주120 min시혈청NO수평강저、ET-1수평승고(t치분별위4.96、4.69、19.04화3.35,P＜0.05).여CC조비교,CI조NO수평강저、ET-1수평승고,차이구유통계학차이(F=18.07、11.97,P＜0.05)；여DC조비교,DI조NO수평강저、ET-1수평승고구유통계학차이(F=4.15、8.04,P＜0.05).DC조기출상태시혈청ET-1수평고우CC조,구유통계학차이(=9.47,P＜0.05).결론 혈청중NO、ET-1재2형당뇨병대서심기결혈재관주손상적병리생리과정중구유일정적의의.
Objective To investigate the roles of endothelin(ET)-1 and nitric oxide(NO) in the myocardial ischemia-reperfusion injury(MIRI) in type 2 diabetic rats.Methods Develop a rat model by feeding the male wistar rats,weighing 160 ～ 180g,with high-fat diet following low-dose STZ as type 2 diabetic rats.14 diabetic rats were randomly divided into two groups (n =7):diabetic MIRI group (DI),diabetic sham-operative group(DC).14 age matched male wistar rats were randomly divided into two groups (n =7):MIRI group (CI),sham-operative group(CC).After 30 minutes of ischemia,the myocardium was reperfused for 120 minutes.Under basal state and the moment after 120 minutes reperfusion,the contents of NO and ET-1 in the serum were measured.Results Compared with the basal level,The content of NO was decreased and ET-1 was increased in the serum at 120 minutes after reperfusion in CI,DI group (t =4.96,4.69,19.04,3.35,P ＜0.05) ; At the time of 120 minutes after repedfusion,Compared with CC group,the level of NO was decreased and ET-1 was increased in CI group(F =18.07,11.97,P ＜ 0.05) ; Compared with DC group,the level of NO was decreased and ET-1 was increased in DI group(F =4.15,8.04,P ＜0.05).The basal level of NO and ET-1 in group DC was obviously higher than that in group CC (t =9.47,P ＜ 0.05).Conclusion It is suggested that NO and ET-1 might play an important role in the pathogenesis of myocardial ischemia-reperfusion injury in type 2 diabetes rats.