CAJ | 학술논문

目的调查中国北方地区隐源性肝炎及乙肝表面抗原(HBsAg)阴性肝癌患者中隐匿性乙型肝炎病毒(HBV)感染的流行状况.方法收集393个受试者的血清,其中包括隐源性慢性肝炎患者215名、HBsAg阴性肝癌患者178名.使用巢式PCR的方法检测血清中的HBV-DNA,同时使用实时定量PCR的方法检测HBV-DNA的载量.结果在隐源性慢性肝炎患者、HBsAg阴性肝癌患者中隐匿性HBV感染流行率分别为23.7％ (51/215)和68.5％ (122/178).在IgG anti-HBc阳性者中,隐匿性HBV感染率较高.所有隐匿性感染者的病毒载量均较低(＜105拷贝/ml).结论在中国北方隐源性肝炎及肝癌患者中,隐匿性HBV感染率较高.因此,对隐匿性HBV感染应给及足够的重视,避免因输血及器官移植造成HBV的传播.
목적 조사중국북방지구은원성간염급을간표면항원(HBsAg)음성간암환자중은닉성을형간염병독(HBV)감염적류행상황.방법 수집393개수시자적혈청,기중포괄은원성만성간염환자215명、HBsAg음성간암환자178명.사용소식PCR적방법검측혈청중적HBV-DNA,동시사용실시정량PCR적방법검측HBV-DNA적재량.결과 재은원성만성간염환자、HBsAg음성간암환자중은닉성HBV감염류행솔분별위23.7％ (51/215)화68.5％ (122/178).재IgG anti-HBc양성자중,은닉성HBV감염솔교고.소유은닉성감염자적병독재량균교저(＜105고패/ml).결론 재중국북방은원성간염급간암환자중,은닉성HBV감염솔교고.인차,대은닉성HBV감염응급급족구적중시,피면인수혈급기관이식조성HBV적전파.
Objective To investigate the prevalence of occult HBV infection among cryptogenic chronic liver disease patients and hepatitis B surface antigen (HBsAg) negative hepatocellular carcinoma (HCC) patients in north of China.Methods The HBV DNA in 393 sera samples collected from cryptogenic chronic liver disease patients (215),HBsAg negative hepatocellular carcinoma (HCC) patients (178) were tested by nested PCR.We performed real-time PCR to determine the levels of serum HBV-DNA.Results Prevalence of occult HBV infection was 23.7％ (51/215) and 68.5％ (122/178) in cryptogenic chronic liver disease patients,HBsAg-negative HCC patients,respectivly.The prevalence of occult HBV infection in IgG anti-HBc-positive subjects was significantly high.In all cases,viral loads were low (＜ 105 viral copies/ml).Conclusion The prevalence of occult HBV infection was significantly high among cryptogenic chronic liver disease patients and HBsAg-negative HCC patients in north of China.Thus,more meticulous attention should be given to prevent HBV transmission by blood transfusion or organ transplantation in endemic areas,and further studies on clinical implication and mechanism of occult HBV infection are required.