国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2014年
6期
546-548
,共3页
赵鸿鹰%粱颖%裴峪%周海燕%张琪琪%王亚贤
趙鴻鷹%粱穎%裴峪%週海燕%張琪琪%王亞賢
조홍응%량영%배욕%주해연%장기기%왕아현
红花多糖%CD44%自分泌运动因子
紅花多糖%CD44%自分泌運動因子
홍화다당%CD44%자분비운동인자
Safflower polysaccharide%CD44%Autocrine motility factor
目的 研究红花多糖(SPS)对荷瘤小鼠肿瘤转移相关基因CD44和自分泌运动因子(AMF)表达的影响.方法 建立荷S180小鼠模型,利用Real-time PCR法检测小鼠肿瘤组织CD44和AMF mRNA表达情况.结果 SPS低、中、高剂量组荷瘤小鼠肿瘤组织中CD44的2-△△Ct值分别是对照组的0.49倍、0.33倍和0.20倍,AMF的2-△△Ct值分别是模型对照组的0.45倍、0.28倍和0.10倍.结论 SPS能够抑制小鼠瘤组织CD44和AMF mRNA的表达,从而抑制肿瘤的转移.
目的 研究紅花多糖(SPS)對荷瘤小鼠腫瘤轉移相關基因CD44和自分泌運動因子(AMF)錶達的影響.方法 建立荷S180小鼠模型,利用Real-time PCR法檢測小鼠腫瘤組織CD44和AMF mRNA錶達情況.結果 SPS低、中、高劑量組荷瘤小鼠腫瘤組織中CD44的2-△△Ct值分彆是對照組的0.49倍、0.33倍和0.20倍,AMF的2-△△Ct值分彆是模型對照組的0.45倍、0.28倍和0.10倍.結論 SPS能夠抑製小鼠瘤組織CD44和AMF mRNA的錶達,從而抑製腫瘤的轉移.
목적 연구홍화다당(SPS)대하류소서종류전이상관기인CD44화자분비운동인자(AMF)표체적영향.방법 건립하S180소서모형,이용Real-time PCR법검측소서종류조직CD44화AMF mRNA표체정황.결과 SPS저、중、고제량조하류소서종류조직중CD44적2-△△Ct치분별시대조조적0.49배、0.33배화0.20배,AMF적2-△△Ct치분별시모형대조조적0.45배、0.28배화0.10배.결론 SPS능구억제소서류조직CD44화AMF mRNA적표체,종이억제종류적전이.
Objective To study the effect of safflower polysaccharide(SPS) on expression of tumor metastasis related genes CD44 and autocrine motility factor(AMF) mRNA on BALB/c mice bearing S180.Method CD44 and AMF mRNA expression tumor tissue of BALB/c mice was detected by Real-time PCR assay.Result Real-time PCR detection found that the SPS played a role in tumor-bearing mice.CD44 and AMF mRNA expression was decreased in tumor tissues.Conclusion SPS can inhibit the expression of CD44 and AMF mRNA which may lead to the inhibition of tumor metastasis
