国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2013年
1期
7-11
,共5页
延育强%薛荣亮%吕建瑞%滕云鹏%赵紫玉
延育彊%薛榮亮%呂建瑞%滕雲鵬%趙紫玉
연육강%설영량%려건서%등운붕%조자옥
急性等容性血液稀释%全脑缺血/再灌注%细胞色素c氧化酶
急性等容性血液稀釋%全腦缺血/再灌註%細胞色素c氧化酶
급성등용성혈액희석%전뇌결혈/재관주%세포색소c양화매
Acute normovolmic hemodilution%Cerebral ischemia/reperfusion%Cytochrome c oxidase
目的 从线粒体功能方面探讨急性等容性血液稀释(acute normovolemic hemodilution,ANH)对全脑缺血/再灌注(ischemia/reperfusion,I/R)损伤大鼠的保护作用.方法 24只雄性清洁SD大鼠,按随机数字表法随机分为4组:假手术组(SHAM组),I/R组,血液稀释至红细胞压积(hematocrit,Hct)30％后I/R组(HES30组),血液稀释至Hct40％后I/R组(HES40组),每组6只.采用四血管法建立大鼠全脑I/R模型.夹闭双侧颈总动脉前0.5 h,从股静脉抽取血液,同时输注等量6％羟乙基淀粉130/0.4氯化钠溶液(hydroxyethyl starch 130/0.4 and sodium chloride injection,HES)行ANH.48 h后处死大鼠,取大脑海马组织,分光光度仪测定细胞色素c氧化酶(cytochrome c oxidase,CcO)活性,逆转录聚合酶链式反应法(reverse transcriptionpolymerase chain reaction,RT-PCR)测定CcO亚基Ⅱ(CcOⅡ)mRSA表达情况.结果 I/R组CcO活性和CcOⅡmRNA表达分别为(0.021±0.012)和(0.079±0.009) (P＜0.01),与SHAM组比较分别降低至约7.580％和20.570％；HES40组CcO活性和CcOⅡmRNA表达分别为(0.120±0.032)和(0.180±0.033)(P＜0.01),与SHAM组比较,分别降低至约43.320％和46.870％；HES30组CcO活性和CcOⅡmRNA表达分别为(0.180±0.026)和(0.347±0.027)(P＜0.01),与SHAM组比较,分别降低至约64.980％和90.360％,与HES40组比较,下降程度较低(P＜0.01).结论 应用6％HES130/0.4 ANH处理全脑I/R能升高线粒体内膜蛋白CcO活性及表达,且在Hct30％时达到最佳效果.适当的ANH处理促进线粒体功能和供能状态改善,具有脑保护作用.
目的 從線粒體功能方麵探討急性等容性血液稀釋(acute normovolemic hemodilution,ANH)對全腦缺血/再灌註(ischemia/reperfusion,I/R)損傷大鼠的保護作用.方法 24隻雄性清潔SD大鼠,按隨機數字錶法隨機分為4組:假手術組(SHAM組),I/R組,血液稀釋至紅細胞壓積(hematocrit,Hct)30％後I/R組(HES30組),血液稀釋至Hct40％後I/R組(HES40組),每組6隻.採用四血管法建立大鼠全腦I/R模型.夾閉雙側頸總動脈前0.5 h,從股靜脈抽取血液,同時輸註等量6％羥乙基澱粉130/0.4氯化鈉溶液(hydroxyethyl starch 130/0.4 and sodium chloride injection,HES)行ANH.48 h後處死大鼠,取大腦海馬組織,分光光度儀測定細胞色素c氧化酶(cytochrome c oxidase,CcO)活性,逆轉錄聚閤酶鏈式反應法(reverse transcriptionpolymerase chain reaction,RT-PCR)測定CcO亞基Ⅱ(CcOⅡ)mRSA錶達情況.結果 I/R組CcO活性和CcOⅡmRNA錶達分彆為(0.021±0.012)和(0.079±0.009) (P＜0.01),與SHAM組比較分彆降低至約7.580％和20.570％；HES40組CcO活性和CcOⅡmRNA錶達分彆為(0.120±0.032)和(0.180±0.033)(P＜0.01),與SHAM組比較,分彆降低至約43.320％和46.870％；HES30組CcO活性和CcOⅡmRNA錶達分彆為(0.180±0.026)和(0.347±0.027)(P＜0.01),與SHAM組比較,分彆降低至約64.980％和90.360％,與HES40組比較,下降程度較低(P＜0.01).結論 應用6％HES130/0.4 ANH處理全腦I/R能升高線粒體內膜蛋白CcO活性及錶達,且在Hct30％時達到最佳效果.適噹的ANH處理促進線粒體功能和供能狀態改善,具有腦保護作用.
목적 종선립체공능방면탐토급성등용성혈액희석(acute normovolemic hemodilution,ANH)대전뇌결혈/재관주(ischemia/reperfusion,I/R)손상대서적보호작용.방법 24지웅성청길SD대서,안수궤수자표법수궤분위4조:가수술조(SHAM조),I/R조,혈액희석지홍세포압적(hematocrit,Hct)30％후I/R조(HES30조),혈액희석지Hct40％후I/R조(HES40조),매조6지.채용사혈관법건립대서전뇌I/R모형.협폐쌍측경총동맥전0.5 h,종고정맥추취혈액,동시수주등량6％간을기정분130/0.4록화납용액(hydroxyethyl starch 130/0.4 and sodium chloride injection,HES)행ANH.48 h후처사대서,취대뇌해마조직,분광광도의측정세포색소c양화매(cytochrome c oxidase,CcO)활성,역전록취합매련식반응법(reverse transcriptionpolymerase chain reaction,RT-PCR)측정CcO아기Ⅱ(CcOⅡ)mRSA표체정황.결과 I/R조CcO활성화CcOⅡmRNA표체분별위(0.021±0.012)화(0.079±0.009) (P＜0.01),여SHAM조비교분별강저지약7.580％화20.570％；HES40조CcO활성화CcOⅡmRNA표체분별위(0.120±0.032)화(0.180±0.033)(P＜0.01),여SHAM조비교,분별강저지약43.320％화46.870％；HES30조CcO활성화CcOⅡmRNA표체분별위(0.180±0.026)화(0.347±0.027)(P＜0.01),여SHAM조비교,분별강저지약64.980％화90.360％,여HES40조비교,하강정도교저(P＜0.01).결론 응용6％HES130/0.4 ANH처리전뇌I/R능승고선립체내막단백CcO활성급표체,차재Hct30％시체도최가효과.괄당적ANH처리촉진선립체공능화공능상태개선,구유뇌보호작용.
Objective This study was designed to discuss the influence of the acute normovolemic hemodilution (ANH) in global cerebral ischemia/reperfusion (I/R) injury in terms of mitochondrial inner membrane.Methods 24 healthy male SD rats were randomly divided into 4 groups(each one 6 rats):sham group(SHAM),I/R group,ANH Hct30％ and I/R group(HES30),ANH Hct40％ and I/R group (HES40).Ischmia was established by four-vessel cerebral I/R model.Half an hour before bilateral carotid arteries were blocked,blood was drawn from the femoral vein,and in the meantime,the same amount of 6％ hydroxyethyl starch (HES) solotion (130/0.4) was transfused.After 48 h,the rats were executed and hippocampus was taken out.The activity of cytochrome c oxidase(CcO) were measured by spectro photometry instrument,and the expression of CcO Ⅱ mRNA were determianted by RT-PCR method.Results Compared with the SHAM group,the CcO activity and CcO Ⅱ mRNA expression of I/R group were reduced to about 7.58％ and 20.57％(0.0210x±0.012 and 0.079±0.009,P＜0.01).In HES40 group,they were reduced to about 43.32％and 46.87％(0.120±0.032 and 0.180±0.033,P＜0.01),compared with the SHAM group.In HES30 group they were reduced to about 64.980％ and 90.360％(0.180±0.026 and 0.347±0.027,P＜0.01) compared with the SHAM group and decreased less than HES40 group.Conclusions Application of 6％HES130/0.4 treatment of ANH can improve activity and expression of CcO in hippocampus mitochondria after cerebral I/R.Its protection achieves the optimal outcome when hemodiluted to Hct30％.The appropriate acute hemodilution elevates themitochondrial function and energy supply,improving the outcome of the global cerebral I/R.
