国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2013年
3期
198-201,215
,共5页
胡正权%吴刘萍%马正良%顾小萍
鬍正權%吳劉萍%馬正良%顧小萍
호정권%오류평%마정량%고소평
氟比洛芬酯%肾功能%凝血功能%血小板聚集
氟比洛芬酯%腎功能%凝血功能%血小闆聚集
불비락분지%신공능%응혈공능%혈소판취집
Flurbiprofen axetil%Kidney%Platelet aggregation%Coagulation function
目的 连续7d腹腔注射氟比洛芬酯注射液,研究其对骨癌痛大鼠肾脏、凝血功能、血小板聚集影响. 方法 36只成熟雌性SD大鼠完全随机分为6组:肿瘤+生理盐水组(C组)、肿瘤+氟比洛芬酯10 mg· kg1·d-1组(CK10组)、肿瘤+氟比洛芬酯25 mg·kg-1·d-1组(CK25组)、肿瘤+氟比洛芬酯50 mg· kg-1·d-1组(CK50组)、氟比洛芬酯50 mg· kg-1·d-1单纯组(K50组)和假手术组+生理盐水组(sham组),每组6只.制作骨癌痛模型14 d后,每天分别腹腔注射相应剂量氟比洛芬酯或生理盐水2次,连续7d后处死大鼠.腹主动脉采血,检测血尿素氮(blood urea nitrogen,BUN)、肌酐(creatinine,Cr)、Na+、K+、凝血酶原时间(protrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)、纤维蛋白原(fibrinogen,Fib)、血小板聚集功能(platelet aggregation,PA)及其观察肾病理学变化.结果 腹腔给予氟比洛芬酯7d后,分别与sham组和C组比较,CK50组(9.9±1.5) mmol/L、K50组(9.7±1.4)mmol/L血BUN水平明显增高(P＜0.05),CK50组(137±8) mmol/L、K50组(138±8)mmol/L Na+和CK50组(3.9±0.3)mmol/L、K50组(3.9±0.4) mmol/L K+显著降低(P＜0.05),Cr、PT、APTT、Fib和PA值变化无统计学意义(P＞0.05)；CK10、CK25组血BUN、Cr、PT、APTT、Fib、Na+、K+和PA值分别与sham组、C组比较,差异无统计学意义(P＞0.05).CK50、K50组病理学变化为肾小球缩小,肾小球毛细血管充盈不足；C组、sham组、CK10、CK25组肾组织结构清晰,未见异常变化. 结论 腹腔重复注射不同剂量氟比洛芬酯对骨肿瘤大鼠凝血功能及其血小板聚集功能无明显影响,然而大剂量氟比洛芬酯(50 mg·kg-1·d-1)影响尿素氮、钠钾离子的代谢及肾小球毛细血管充盈.
目的 連續7d腹腔註射氟比洛芬酯註射液,研究其對骨癌痛大鼠腎髒、凝血功能、血小闆聚集影響. 方法 36隻成熟雌性SD大鼠完全隨機分為6組:腫瘤+生理鹽水組(C組)、腫瘤+氟比洛芬酯10 mg· kg1·d-1組(CK10組)、腫瘤+氟比洛芬酯25 mg·kg-1·d-1組(CK25組)、腫瘤+氟比洛芬酯50 mg· kg-1·d-1組(CK50組)、氟比洛芬酯50 mg· kg-1·d-1單純組(K50組)和假手術組+生理鹽水組(sham組),每組6隻.製作骨癌痛模型14 d後,每天分彆腹腔註射相應劑量氟比洛芬酯或生理鹽水2次,連續7d後處死大鼠.腹主動脈採血,檢測血尿素氮(blood urea nitrogen,BUN)、肌酐(creatinine,Cr)、Na+、K+、凝血酶原時間(protrombin time,PT)、活化部分凝血活酶時間(activated partial thromboplastin time,APTT)、纖維蛋白原(fibrinogen,Fib)、血小闆聚集功能(platelet aggregation,PA)及其觀察腎病理學變化.結果 腹腔給予氟比洛芬酯7d後,分彆與sham組和C組比較,CK50組(9.9±1.5) mmol/L、K50組(9.7±1.4)mmol/L血BUN水平明顯增高(P＜0.05),CK50組(137±8) mmol/L、K50組(138±8)mmol/L Na+和CK50組(3.9±0.3)mmol/L、K50組(3.9±0.4) mmol/L K+顯著降低(P＜0.05),Cr、PT、APTT、Fib和PA值變化無統計學意義(P＞0.05)；CK10、CK25組血BUN、Cr、PT、APTT、Fib、Na+、K+和PA值分彆與sham組、C組比較,差異無統計學意義(P＞0.05).CK50、K50組病理學變化為腎小毬縮小,腎小毬毛細血管充盈不足；C組、sham組、CK10、CK25組腎組織結構清晰,未見異常變化. 結論 腹腔重複註射不同劑量氟比洛芬酯對骨腫瘤大鼠凝血功能及其血小闆聚集功能無明顯影響,然而大劑量氟比洛芬酯(50 mg·kg-1·d-1)影響尿素氮、鈉鉀離子的代謝及腎小毬毛細血管充盈.
목적 련속7d복강주사불비락분지주사액,연구기대골암통대서신장、응혈공능、혈소판취집영향. 방법 36지성숙자성SD대서완전수궤분위6조:종류+생리염수조(C조)、종류+불비락분지10 mg· kg1·d-1조(CK10조)、종류+불비락분지25 mg·kg-1·d-1조(CK25조)、종류+불비락분지50 mg· kg-1·d-1조(CK50조)、불비락분지50 mg· kg-1·d-1단순조(K50조)화가수술조+생리염수조(sham조),매조6지.제작골암통모형14 d후,매천분별복강주사상응제량불비락분지혹생리염수2차,련속7d후처사대서.복주동맥채혈,검측혈뇨소담(blood urea nitrogen,BUN)、기항(creatinine,Cr)、Na+、K+、응혈매원시간(protrombin time,PT)、활화부분응혈활매시간(activated partial thromboplastin time,APTT)、섬유단백원(fibrinogen,Fib)、혈소판취집공능(platelet aggregation,PA)급기관찰신병이학변화.결과 복강급여불비락분지7d후,분별여sham조화C조비교,CK50조(9.9±1.5) mmol/L、K50조(9.7±1.4)mmol/L혈BUN수평명현증고(P＜0.05),CK50조(137±8) mmol/L、K50조(138±8)mmol/L Na+화CK50조(3.9±0.3)mmol/L、K50조(3.9±0.4) mmol/L K+현저강저(P＜0.05),Cr、PT、APTT、Fib화PA치변화무통계학의의(P＞0.05)；CK10、CK25조혈BUN、Cr、PT、APTT、Fib、Na+、K+화PA치분별여sham조、C조비교,차이무통계학의의(P＞0.05).CK50、K50조병이학변화위신소구축소,신소구모세혈관충영불족；C조、sham조、CK10、CK25조신조직결구청석,미견이상변화. 결론 복강중복주사불동제량불비락분지대골종류대서응혈공능급기혈소판취집공능무명현영향,연이대제량불비락분지(50 mg·kg-1·d-1)영향뇨소담、납갑리자적대사급신소구모세혈관충영.
Objective To investigate influences of flurbiprofen axetil on kidney,platelet aggregation and coagulation function.Methods Thirty-six healthy female Sprague Drawley (SD)rats were randomly divided into six groups (6 rats in each group):cancer+normal saline group(CN),cancer+flurbiprofen axetil 10 mg/kg group(CK10),cancer+flurbiprofen axetil 25 mg/kg group (CK25),cancer+ flurbiprofen axetil 50 mg/kg group (CK50),flurbiprofen axetil 50 mg/kg group (K50),and sham+normal saline(sham).Flurbiprofen axetil or normal saline was administered intraperitoneally twice a day from day 14 to day 21 after injection of Walker256 tumour cells into one tibia.Rats were sacrificed to obtain blood from abdominal aorta on the 21th day.Then the levels of blood urea nitrogen (BUN),creatinine (Cr),sodion,Potassium ion,protrombin time(PT),activated partial thromboplastin time (APTF),fibrinogen (Fib),platelet aggregation (PA) were detected and pathological changes of kidney were observed.Results Respectively compared with the sham and C groups,the levels of BUN in the CK50 (9.9 ± 1.5) mmol/L and K50 (9.7±1.4) mmol/Lgroups increased obviously 7 days after intraperitoneal injection of flurbiprofen axetil (P＜0.05),and the levels of sodion in the CK50(137±8) mmol/L and K50 (138±8) mmol/L groups and potassium ion in the CK50 (3.9±0.3) mmol/Land K50 (3.9±0.4) mmol/Lgroups reduced significantly (P＜0.05),and the levels of Cr,PT,APTT,Fib and PA were no statistical difference (P＞0.05).Respectively compared with the sham and C groups,the levels of Cr,BUN,sodion,Potassium ion,Cr,PT,A PTT,Fib and PA were no statistical difference obviously in the CK10 and CK25 groups(P＞0.05).The main pathological changes in the CK50 and K50groups were glomerular narrow and poor supplement with blood in capillaries,while in the sham,C,CK10 and CK25 groups,there were no pathological changes observed.Conclusions Intraperitoneal injection of flurbiprofen axetil in a certain range of dose was not found to impair platelet aggregation and coagulation function in a model of bone cancer pain in the rat.However,in large dose,there were obviously influences on the metabolism of BUN,sodion and potassium ion as well asinfusion of capillaries of renal glomeruli in rats.
