国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2013年
5期
390-394
,共5页
脂联素%脓毒症%血红素氧化酶-1%肺损伤
脂聯素%膿毒癥%血紅素氧化酶-1%肺損傷
지련소%농독증%혈홍소양화매-1%폐손상
Adiponectin%Sepsis%Heme oxygenase-1%Lung injury
目的 探讨血红素氧化酶-1(heme oxygenase,HO-1)介导脂联素(adiponectin,APN)在脓毒症肺损伤中的保护作用.方法 80只雄性Wistar大鼠采用随机数字表法分为4组(每组20只):对照组(C组)、脓毒症模型组(LPS组)、脂联素预处理组(APN组)和HO-1抑制剂锌原卟啉IX(zinc protoporphyrin IX,ZnPP IX)干预组(Zn组).各组分别于腹腔注射脂多糖(lipopolysaccharide,LPS) (20 mg/kg)或0.9％氯化钠溶液后6h,采用酶联免疫吸附法检测血清中肿瘤坏死因子(tumor necrosis factor-alpha,TNF-α)和白介素(interlukin-6,IL-6)的含量.采用分光光度法测定一氧化氮(nitric oxide,NO)浓度、肺组织丙二醛(malonaldehyde,MDA)和髓过氧化物酶(myeloperoxidase,MPO)活性变化.采用实时荧光定量聚合酶链反应(real-time PCR)法检测肺组织HO-1、诱导型一氧化氮合成酶(inducible nitric oxide synthase,iNOS)mRNA的表达水平.观察5d内大鼠生存情况,并绘制生存曲线. 结果 与C组比较,LPS组、APN组和Zn组血清TNF-α [(321.3±11.2)、(132.5±10.2)、(311.7±12.4)vs (52.1±1.4)] ng/L、IL-6[(2 229.26±210.25)、(1 134.14±11.24)、(2 028.27±167.04)vs(55.38±0.23)] ng/L、NO[(103±10)、(79±8)、(97±9)vs(48±3)] μmol/L和肺组织匀浆MDA[(16.209±0.151) 、(5.943±0.310)、(13.238±0.326)vs(3.081 ±0.017)] μmol/g、MPO[(12.42±0.46)、(6.77±0.14)、(10.45±0.21)vs(2.74±0.06)] U/g水平显著升高(P＜0.05),生存率显著下降(20％ vs 100％;70％ vs 100％;30％ vs 100％,P＜0.05);与LPS组比较,APN组血清TNF-α [(132.5±10.2)vs (321.3±11.2)] ng/L、IL-6 [(1 134.14±107.13)vs(2229.26±210.25)] ng/L、NO[(79±8)vs (103±10)] μmol/L和肺组织匀浆MDA[(5.943±0.310)vs (16.209±0.151)] μmol/g、MPO [(6.77±0.14)vs (12.42±0.46)] U/g水平显著降低(P＜0.05),肺组织HO-1 mRNA[(8.73±0.24)vs(1.54±0.03)]显著升高(P＜0.05),iNOS mRNA[(1.42±0.15)vs(2.01±0.10)]显著下降(P＜0.05),生存率显著增高(70％ vs 20％,P＜0.05);与APN组比较,Zn组血清TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L、IL-6[(2 028.27±167.04)vs(1 134.14±107.13)]ng/L、NO[(97±9)vs(79±8)] μmol/L和肺组织匀浆MDA[(13.238±0.326)vs(5.943±0.310)] μmol/g、MPO[(10.45±0.21)vs(6.77±0.14)] U/g水平显著升高(P＜0.05),肺组织HO-1 mRNA[(2.66±0.14)vs (8.73±0.24)]显著下降(P＜0.05),iNOS mRNA[(2.06±0.23)vs(1.42±0.15)]显著升高(P＜0.05),生存率显著下降(30％ vs 70％,P＜0.05). 结论 HO-1可能介导了APN在脓毒症性肺损伤中的保护作用.
目的 探討血紅素氧化酶-1(heme oxygenase,HO-1)介導脂聯素(adiponectin,APN)在膿毒癥肺損傷中的保護作用.方法 80隻雄性Wistar大鼠採用隨機數字錶法分為4組(每組20隻):對照組(C組)、膿毒癥模型組(LPS組)、脂聯素預處理組(APN組)和HO-1抑製劑鋅原卟啉IX(zinc protoporphyrin IX,ZnPP IX)榦預組(Zn組).各組分彆于腹腔註射脂多糖(lipopolysaccharide,LPS) (20 mg/kg)或0.9％氯化鈉溶液後6h,採用酶聯免疫吸附法檢測血清中腫瘤壞死因子(tumor necrosis factor-alpha,TNF-α)和白介素(interlukin-6,IL-6)的含量.採用分光光度法測定一氧化氮(nitric oxide,NO)濃度、肺組織丙二醛(malonaldehyde,MDA)和髓過氧化物酶(myeloperoxidase,MPO)活性變化.採用實時熒光定量聚閤酶鏈反應(real-time PCR)法檢測肺組織HO-1、誘導型一氧化氮閤成酶(inducible nitric oxide synthase,iNOS)mRNA的錶達水平.觀察5d內大鼠生存情況,併繪製生存麯線. 結果 與C組比較,LPS組、APN組和Zn組血清TNF-α [(321.3±11.2)、(132.5±10.2)、(311.7±12.4)vs (52.1±1.4)] ng/L、IL-6[(2 229.26±210.25)、(1 134.14±11.24)、(2 028.27±167.04)vs(55.38±0.23)] ng/L、NO[(103±10)、(79±8)、(97±9)vs(48±3)] μmol/L和肺組織勻漿MDA[(16.209±0.151) 、(5.943±0.310)、(13.238±0.326)vs(3.081 ±0.017)] μmol/g、MPO[(12.42±0.46)、(6.77±0.14)、(10.45±0.21)vs(2.74±0.06)] U/g水平顯著升高(P＜0.05),生存率顯著下降(20％ vs 100％;70％ vs 100％;30％ vs 100％,P＜0.05);與LPS組比較,APN組血清TNF-α [(132.5±10.2)vs (321.3±11.2)] ng/L、IL-6 [(1 134.14±107.13)vs(2229.26±210.25)] ng/L、NO[(79±8)vs (103±10)] μmol/L和肺組織勻漿MDA[(5.943±0.310)vs (16.209±0.151)] μmol/g、MPO [(6.77±0.14)vs (12.42±0.46)] U/g水平顯著降低(P＜0.05),肺組織HO-1 mRNA[(8.73±0.24)vs(1.54±0.03)]顯著升高(P＜0.05),iNOS mRNA[(1.42±0.15)vs(2.01±0.10)]顯著下降(P＜0.05),生存率顯著增高(70％ vs 20％,P＜0.05);與APN組比較,Zn組血清TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L、IL-6[(2 028.27±167.04)vs(1 134.14±107.13)]ng/L、NO[(97±9)vs(79±8)] μmol/L和肺組織勻漿MDA[(13.238±0.326)vs(5.943±0.310)] μmol/g、MPO[(10.45±0.21)vs(6.77±0.14)] U/g水平顯著升高(P＜0.05),肺組織HO-1 mRNA[(2.66±0.14)vs (8.73±0.24)]顯著下降(P＜0.05),iNOS mRNA[(2.06±0.23)vs(1.42±0.15)]顯著升高(P＜0.05),生存率顯著下降(30％ vs 70％,P＜0.05). 結論 HO-1可能介導瞭APN在膿毒癥性肺損傷中的保護作用.
목적 탐토혈홍소양화매-1(heme oxygenase,HO-1)개도지련소(adiponectin,APN)재농독증폐손상중적보호작용.방법 80지웅성Wistar대서채용수궤수자표법분위4조(매조20지):대조조(C조)、농독증모형조(LPS조)、지련소예처리조(APN조)화HO-1억제제자원계람IX(zinc protoporphyrin IX,ZnPP IX)간예조(Zn조).각조분별우복강주사지다당(lipopolysaccharide,LPS) (20 mg/kg)혹0.9％록화납용액후6h,채용매련면역흡부법검측혈청중종류배사인자(tumor necrosis factor-alpha,TNF-α)화백개소(interlukin-6,IL-6)적함량.채용분광광도법측정일양화담(nitric oxide,NO)농도、폐조직병이철(malonaldehyde,MDA)화수과양화물매(myeloperoxidase,MPO)활성변화.채용실시형광정량취합매련반응(real-time PCR)법검측폐조직HO-1、유도형일양화담합성매(inducible nitric oxide synthase,iNOS)mRNA적표체수평.관찰5d내대서생존정황,병회제생존곡선. 결과 여C조비교,LPS조、APN조화Zn조혈청TNF-α [(321.3±11.2)、(132.5±10.2)、(311.7±12.4)vs (52.1±1.4)] ng/L、IL-6[(2 229.26±210.25)、(1 134.14±11.24)、(2 028.27±167.04)vs(55.38±0.23)] ng/L、NO[(103±10)、(79±8)、(97±9)vs(48±3)] μmol/L화폐조직균장MDA[(16.209±0.151) 、(5.943±0.310)、(13.238±0.326)vs(3.081 ±0.017)] μmol/g、MPO[(12.42±0.46)、(6.77±0.14)、(10.45±0.21)vs(2.74±0.06)] U/g수평현저승고(P＜0.05),생존솔현저하강(20％ vs 100％;70％ vs 100％;30％ vs 100％,P＜0.05);여LPS조비교,APN조혈청TNF-α [(132.5±10.2)vs (321.3±11.2)] ng/L、IL-6 [(1 134.14±107.13)vs(2229.26±210.25)] ng/L、NO[(79±8)vs (103±10)] μmol/L화폐조직균장MDA[(5.943±0.310)vs (16.209±0.151)] μmol/g、MPO [(6.77±0.14)vs (12.42±0.46)] U/g수평현저강저(P＜0.05),폐조직HO-1 mRNA[(8.73±0.24)vs(1.54±0.03)]현저승고(P＜0.05),iNOS mRNA[(1.42±0.15)vs(2.01±0.10)]현저하강(P＜0.05),생존솔현저증고(70％ vs 20％,P＜0.05);여APN조비교,Zn조혈청TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L、IL-6[(2 028.27±167.04)vs(1 134.14±107.13)]ng/L、NO[(97±9)vs(79±8)] μmol/L화폐조직균장MDA[(13.238±0.326)vs(5.943±0.310)] μmol/g、MPO[(10.45±0.21)vs(6.77±0.14)] U/g수평현저승고(P＜0.05),폐조직HO-1 mRNA[(2.66±0.14)vs (8.73±0.24)]현저하강(P＜0.05),iNOS mRNA[(2.06±0.23)vs(1.42±0.15)]현저승고(P＜0.05),생존솔현저하강(30％ vs 70％,P＜0.05). 결론 HO-1가능개도료APN재농독증성폐손상중적보호작용.
Objective To investigate the protective effect of adiponectin (APN) on lung injury in LPS-induced septic rats and whether the protective effect is mediated by heme oxygenase-1 (HO-1).Methods 80 Wistar male rats were randomly divided into 4 groups:control group(C group),LPS group,adiponectin treatment group(APN group) and zinc protoporphyrin IX (ZnPP IX) treatment group (Zn group).At 6h after intraperitoneal injection of lipopolysaccharide (LPS),the serum levels of tumor necrosis factor (TNF)-α and interlukin (IL-6) were detected by ELISA.The serum level of nitric oxide (NO),malonodialdehyde (MDA)content and myeloperoxidase (MPO) activity in lung tissue were detected by spectrophptometry.Lung HO-1,inducible nitric oxide synthase (iNOS) mRNA were detected with real time pohymerase chain reaction (RT-PCR).The mortality rate was calculated 7 d after operation and the survival curves were drawn.Results The serum levels of TNF-α [(321.3±11.2),(132.5±10.2),(311.7± 12.4)vs(52.1±1.4)] ng/L,IL-6[(2 229.26±210.25),(1 134.14±11.24),(2 028.27±167.04)vs(55.38±0.23)] ng/L and NO[(103±10),(79±8),(97±9) vs (48±3)] μmol/L in LPS,APN,Zn groups were higher than those in C group (P＜0.05).The MDA concentration [(16.209±0.151),(5.943±0.310),(13.238±0.326) vs (3.081±0.017)] μmol/g and MPO activity [(12.42±0.46),(6.77±0.14),(10.45±0.21)vs (2.74±0.06)] U/g in lung homogenate in LPS group were higher than those in C group (P＜0.05).The survival rate of rats in LPS,APN,Zn groups were lower than that of C group (20％ vs 100％,70％ vs 100％,30％ vs 100％,P＜0.05).The serum levels of TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L,IL-6 [(1 134.14±107.13)vs(2 229.26±210.25)] ng/L and NO[(79±8)vs(103± 10)] μmol/L in APN group were decreased as compared with LPS group (P＜0.05).The MDA concentration [(5.943±0.310)vs (16.209±0.151)] μmol/g and MPO activity[(6.77±0.14)vs(12.42±0.46)] U/g in lung homogenate and the lung iNOS mRNA [(1.42±0.15)vs(2.01±0.10)] in APN group were decreased as compared with LPS group (P＜0.05).The lung HO-1 mRNA in APN group was higher than that in LPS group [(8.73±0.24)vs (1.54±0.03)(P＜0.05)].The survival rate of rats in APN group was lower than that of LPS group (70％ vs 20％,P＜0.05).The serum levels of TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L,IL-6[(2 028.27±167.04) vs (1134.14±107.13)] ng/L and NO[(97±9)vs(79±8)] μmol/L in Zn group were increased as compared with APN group (P＜0.05).The MDA concentration [(13.238±0.326)vs (5.943±0.310)] μ mol/g and MPO activity [(10.45±0.21)vs (6.77±0.14)] U/g in lung homogenate and the lung iNOS mRNA[(2.06±0.23)vs(1.42±0.15)] in Zn group were raised as compared with APN group (P＜0.05).The lung HO-1 mRNA [(2.66±0.14)vs(8.73±0.24)] in group Zn was decreased as compared with APN group(P＜0.05).The survival rate of rats in Zn group was lower than that of APN group (30％ vs 70％,P＜0.05).Conclusions HO-1 may mediate the protective effect of adiponectin on lung injury in LPS-induced septic rats.concentration [(16.209±0.151),(5.943±0.310),(13.238±0.326) vs (3.081±0.017)] μmol/g and MPO activity [(12.42±0.46),(6.77±0.14),(10.45±0.21)vs (2.74±0.06)] U/g in lung homogenate in LPS group were higher than those in C group (P＜0.05).The survival rate of rats in LPS,APN,Zn groups were lower than that of C group (20％ vs 100％,70％ vs 100％,30％ vs 100％,P＜0.05).The serum levels of TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L,IL-6 [(1 134.14±107.13)vs(2 229.26±210.25)] ng/L and NO[(79±8)vs(103± 10)] μmol/L in APN group were decreased as compared with LPS group (P＜0.05).The MDA concentration [(5.943±0.310)vs (16.209±0.151)] μmol/g and MPO activity[(6.77±0.14)vs(12.42±0.46)] U/g in lung homogenate and the lung iNOS mRNA [(1.42±0.15)vs(2.01±0.10)] in APN group were decreased as compared with LPS group (P＜0.05).The lung HO-1 mRNA in APN group was higher than that in LPS group [(8.73±0.24)vs (1.54±0.03)(P＜0.05)].The survival rate of rats in APN group was lower than that of LPS group (70％ vs 20％,P＜0.05).The serum levels of TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L,IL-6[(2 028.27±167.04) vs (1134.14±107.13)] ng/L and NO[(97±9)vs(79±8)] μmol/L in Zn group were increased as compared with APN group (P＜0.05).The MDA concentration [(13.238±0.326)vs (5.943±0.310)] μ mol/g and MPO activity [(10.45±0.21)vs (6.77±0.14)] U/g in lung homogenate and the lung iNOS mRNA[(2.06±0.23)vs(1.42±0.15)] in Zn group were raised as compared with APN group (P＜0.05).The lung HO-1 mRNA [(2.66±0.14)vs(8.73±0.24)] in group Zn was decreased as compared with APN group(P＜0.05).The survival rate of rats in Zn group was lower than that of APN group (30％ vs 70％,P＜0.05).Conclusions HO-1 may mediate the protective effect of adiponectin on lung injury in LPS-induced septic rats.concentration [(16.209±0.151),(5.943±0.310),(13.238±0.326) vs (3.081±0.017)] μmol/g and MPO activity [(12.42±0.46),(6.77±0.14),(10.45±0.21)vs (2.74±0.06)] U/g in lung homogenate in LPS group were higher than those in C group (P＜0.05).The survival rate of rats in LPS,APN,Zn groups were lower than that of C group (20％ vs 100％,70％ vs 100％,30％ vs 100％,P＜0.05).The serum levels of TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L,IL-6 [(1 134.14±107.13)vs(2 229.26±210.25)] ng/L and NO[(79±8)vs(103± 10)] μmol/L in APN group were decreased as compared with LPS group (P＜0.05).The MDA concentration [(5.943±0.310)vs (16.209±0.151)] μmol/g and MPO activity[(6.77±0.14)vs(12.42±0.46)] U/g in lung homogenate and the lung iNOS mRNA [(1.42±0.15)vs(2.01±0.10)] in APN group were decreased as compared with LPS group (P＜0.05).The lung HO-1 mRNA in APN group was higher than that in LPS group [(8.73±0.24)vs (1.54±0.03)(P＜0.05)].The survival rate of rats in APN group was lower than that of LPS group (70％ vs 20％,P＜0.05).The serum levels of TNF-α[(311.7±12.4)vs(132.5±10.2)] ng/L,IL-6[(2 028.27±167.04) vs (1134.14±107.13)] ng/L and NO[(97±9)vs(79±8)] μmol/L in Zn group were increased as compared with APN group (P＜0.05).The MDA concentration [(13.238±0.326)vs (5.943±0.310)] μ mol/g and MPO activity [(10.45±0.21)vs (6.77±0.14)] U/g in lung homogenate and the lung iNOS mRNA[(2.06±0.23)vs(1.42±0.15)] in Zn group were raised as compared with APN group (P＜0.05).The lung HO-1 mRNA [(2.66±0.14)vs(8.73±0.24)] in group Zn was decreased as compared with APN group(P＜0.05).The survival rate of rats in Zn group was lower than that of APN group (30％ vs 70％,P＜0.05).Conclusions HO-1 may mediate the protective effect of adiponectin on lung injury in LPS-induced septic rats.
