国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2013年
9期
787-789,798
,共4页
梁启胜%杨茗峻%徐宁%陶静%陈菲菲%梁伟晨
樑啟勝%楊茗峻%徐寧%陶靜%陳菲菲%樑偉晨
량계성%양명준%서저%도정%진비비%량위신
七氟醚%缺血/再灌注%损伤%c-Fos蛋白
七氟醚%缺血/再灌註%損傷%c-Fos蛋白
칠불미%결혈/재관주%손상%c-Fos단백
Sevoflurane%Ischemia/reperfusion%Injury%c-Fos protein
目的 探讨七氟醚麻醉对兔肝缺血/再灌注损伤(ischemia/reperfusion injury,Ⅰ/RI)的影响. 方法 健康成年新西兰大白兔21只,按照随机数字表法分为3组(每组7例):假手术组(S组)、肝Ⅰ/R组(Ⅰ/R组)、七氟醚麻醉组(Sev-Ⅰ/R组).结扎肝动脉建立兔肝脏Ⅰ/RI模型,分别于缺血前(Tl)、缺血45 min(T2)和再灌注60 min(T3)测定血浆丙氨酸转氨酶(aspartate a minotransferase,AST)及谷草转氨酶(alanine aminotransferase,ALT)水平,于T3测定肝组织丙二醛(malondialdehyde,MDA)含量；免疫组化染色检测c-Fos蛋白的表达情况. 结果 与S组比较,Ⅰ/R组血浆ALT和AST活性[T2:(48±6) U/L和(48±7) U/L,T3:(66±6) U/L和(70±6)U/L]及Sev-Ⅰ/R组血浆ALT和AST活性[T2:(43±7) U/L和(41±3) U/L,T3:(48±6) U/L和(50±6) U/L]、肝组织MDA含量和c-Fos蛋白表达水平均明显升高(P＜0.05或P＜0.01)；Sev-Ⅰ/R组所有这些实验指标均明显低于Ⅰ/R组(P＜0.05或P＜0.01). 结论 七氟醚对Ⅰ/RI兔肝组织有保护作用,可与其降低活性氧产物有关.
目的 探討七氟醚痳醉對兔肝缺血/再灌註損傷(ischemia/reperfusion injury,Ⅰ/RI)的影響. 方法 健康成年新西蘭大白兔21隻,按照隨機數字錶法分為3組(每組7例):假手術組(S組)、肝Ⅰ/R組(Ⅰ/R組)、七氟醚痳醉組(Sev-Ⅰ/R組).結扎肝動脈建立兔肝髒Ⅰ/RI模型,分彆于缺血前(Tl)、缺血45 min(T2)和再灌註60 min(T3)測定血漿丙氨痠轉氨酶(aspartate a minotransferase,AST)及穀草轉氨酶(alanine aminotransferase,ALT)水平,于T3測定肝組織丙二醛(malondialdehyde,MDA)含量；免疫組化染色檢測c-Fos蛋白的錶達情況. 結果 與S組比較,Ⅰ/R組血漿ALT和AST活性[T2:(48±6) U/L和(48±7) U/L,T3:(66±6) U/L和(70±6)U/L]及Sev-Ⅰ/R組血漿ALT和AST活性[T2:(43±7) U/L和(41±3) U/L,T3:(48±6) U/L和(50±6) U/L]、肝組織MDA含量和c-Fos蛋白錶達水平均明顯升高(P＜0.05或P＜0.01)；Sev-Ⅰ/R組所有這些實驗指標均明顯低于Ⅰ/R組(P＜0.05或P＜0.01). 結論 七氟醚對Ⅰ/RI兔肝組織有保護作用,可與其降低活性氧產物有關.
목적 탐토칠불미마취대토간결혈/재관주손상(ischemia/reperfusion injury,Ⅰ/RI)적영향. 방법 건강성년신서란대백토21지,안조수궤수자표법분위3조(매조7례):가수술조(S조)、간Ⅰ/R조(Ⅰ/R조)、칠불미마취조(Sev-Ⅰ/R조).결찰간동맥건립토간장Ⅰ/RI모형,분별우결혈전(Tl)、결혈45 min(T2)화재관주60 min(T3)측정혈장병안산전안매(aspartate a minotransferase,AST)급곡초전안매(alanine aminotransferase,ALT)수평,우T3측정간조직병이철(malondialdehyde,MDA)함량；면역조화염색검측c-Fos단백적표체정황. 결과 여S조비교,Ⅰ/R조혈장ALT화AST활성[T2:(48±6) U/L화(48±7) U/L,T3:(66±6) U/L화(70±6)U/L]급Sev-Ⅰ/R조혈장ALT화AST활성[T2:(43±7) U/L화(41±3) U/L,T3:(48±6) U/L화(50±6) U/L]、간조직MDA함량화c-Fos단백표체수평균명현승고(P＜0.05혹P＜0.01)；Sev-Ⅰ/R조소유저사실험지표균명현저우Ⅰ/R조(P＜0.05혹P＜0.01). 결론 칠불미대Ⅰ/RI토간조직유보호작용,가여기강저활성양산물유관.
Objective To investigate the effects of sevoflurane anesthesia on hepatic ischemia/reperfusion injury (Ⅰ/RI) in rabbits.Methods Twenty-one healthy adult New Zealand white rabbits were randomly divided into three groups (n=7):sham operation group (S group),liver ischemia/reperfusion group (Ⅰ/R group) and sevoflurane group (Sev-Ⅰ/R group).The liver Ⅰ/R injury model was established in rabbit by clamping hepatic artery for 45 min,and then reperfusing for 60 min.Blood samples were collected at before (T1) and 45 min post ischemia (T2) and 60 min post-reperfusion (T3) respectively for measurement of plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT).Liver specimens were obtained at the end of the experiment for determination of malondialdehyde (MDA) content and examination of pathological changes by HE staining.The expressions of cFos protein were observed by immunohistochemisty.Results Compared with S group,plasma ALT and AST activity in Ⅰ/R group [T2(48±6) U/L and (48±7) U/L,T3(66±6) U/L and (70±6) U/L],plasma ALT and AST activity in Sev-Ⅰ/R group [T2(43±7) U/L and T3(41±3) U/L,(48±6) U/L and (50±6) U/L],liver tissue MDA content,and c-Fos expressions in Ⅰ/R group and Sev-Ⅰ/R group all increased significantly (P＜0.05 or P＜0.01).These indexes in Sev-Ⅰ/R group were all lower than those in Ⅰ/R group(P＜0.05 or P＜0.01).Conclusions Sevoflurane could protect the rabbit liver from Ⅰ/RI,and which may be related to the down-regulation of peroxides.
