CAJ | 학술논문

目的探讨高压氧(hyperbaric oxygen,HBO)后处理对大鼠神经病理性疼痛(neuropathic pain,NP)镇痛效果及脊髓小胶质细胞活化的影响. 方法雄性SD大鼠24只,采用随机数字表法将大鼠分成4组(每组6只):假手术组(S组)、坐骨神经慢性压迫组(C组)、HBO后处理2.0组(H2.0组)与HBO后处理2.5组(H2.5组).采用坐骨神经慢性压迫损伤法建立NP模型,H2.0和H2.5组于术后1d开始给予相应压力的HBO后处理,1次/d连续7次,每天出HBO舱后1h测定各组大鼠机械缩足反射阈值(mechanical withdrawal threshold,MWT)和热缩足反射潜伏期(thermal withdrawal latency,TWL),术后7d测定MWT和TWL后处死,用免疫组化法测定大鼠脊髓小胶质细胞的活化情况. 结果与S组比较,C组MWT降低及TWL缩短,脊髓内小胶质细胞数量与活化率上升为(156±9)个和44.9％(P＜0.05)；与C组比较,H2.0组和H2.5组MWT升高及TWL延长,H2.0组和小胶质细胞数量与活化率分别为(64±7)个和5.7％(P＜0.05),H2.5组小胶质细胞数量与活化率分别为(62±5)个和6.1％(P＜0.05)；H2.0组与H2.5组小胶质细胞数量与活化率差异无统计学意义(P＞0.05). 结论 HBO后处理可减轻大鼠NP,其机制与抑制脊髓内小胶质细胞活化有关.
목적 탐토고압양(hyperbaric oxygen,HBO)후처리대대서신경병이성동통(neuropathic pain,NP)진통효과급척수소효질세포활화적영향. 방법 웅성SD대서24지,채용수궤수자표법장대서분성4조(매조6지):가수술조(S조)、좌골신경만성압박조(C조)、HBO후처리2.0조(H2.0조)여HBO후처리2.5조(H2.5조).채용좌골신경만성압박손상법건립NP모형,H2.0화H2.5조우술후1d개시급여상응압력적HBO후처리,1차/d련속7차,매천출HBO창후1h측정각조대서궤계축족반사역치(mechanical withdrawal threshold,MWT)화열축족반사잠복기(thermal withdrawal latency,TWL),술후7d측정MWT화TWL후처사,용면역조화법측정대서척수소효질세포적활화정황. 결과 여S조비교,C조MWT강저급TWL축단,척수내소효질세포수량여활화솔상승위(156±9)개화44.9％(P＜0.05)；여C조비교,H2.0조화H2.5조MWT승고급TWL연장,H2.0조화소효질세포수량여활화솔분별위(64±7)개화5.7％(P＜0.05),H2.5조소효질세포수량여활화솔분별위(62±5)개화6.1％(P＜0.05)；H2.0조여H2.5조소효질세포수량여활화솔차이무통계학의의(P＞0.05). 결론 HBO후처리가감경대서NP,기궤제여억제척수내소효질세포활화유관.
Objective To investigate the antinociceptive effect of hyperbaric oxygen(HBO) on activation of microglia in rats with neuropathic pain (NP) produced by chronic constriction injury of the sciatic nerve.Methods Twenty four healthy male Sprague-Dawley rats were randomly divided into four groups (n=6):Sham group (group S),CCI group (group C),2.0 ATA HBO post-conditioning group (group H2.0) and 2.5ATA HBO post-conditioning group (group H2.5).Group CCI,group H2.0 and group H2.5 were received the surgery of the chronic constriction injury.One day later,surgery HBO of 2.0 ATA and 2.5 ATA was given respectively by hyperbaric oxygen cabin once a day for seven days.The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of rats in each group were measured respectively 1 h after the surgery every day.The rats were killed after 7 days when MWT and TWL had already been measured.The lumbar segment L4-5 of the spinal cord was removed.Immunohistochemical technique was used to detect the activation of microglias in the spinal cord.Results Compared with group S,MWT and TWL were significantly decreased and the activation of microglias in the spinal cord was significantly enhanced after surgery in group C(P＜0.05).Compared with group C,MWT and TWL were significantly increased and the activation of microglias in the spinal cord was significantly inhibited after surgery in group H2.0 and H2.5 (P＜0.05).There was no significant difference in the activation of spinal microglias between group H2.0 and H2.5.Conclusions HBO post-conditioning may produce the antinociceptive effect by inhibiting activation of spinal microglias in rats model of neuropathic pain.