国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2013年
9期
803-807
,共5页
苗蓓%殷悦%周田田%邵翠杰%曹君利
苗蓓%慇悅%週田田%邵翠傑%曹君利
묘배%은열%주전전%소취걸%조군리
加巴喷丁%糖尿病神经病理性痛%背根神经节%Toll样受体4
加巴噴丁%糖尿病神經病理性痛%揹根神經節%Toll樣受體4
가파분정%당뇨병신경병이성통%배근신경절%Toll양수체4
Gabapentin%Diabetic neuropathic pain%Dorsal root ganglia%Toll-like receptor 4
目的 探讨加巴喷丁(gabapentin,GBP)对糖尿病神经病理性疼痛(diabetic neuropathic pain,DNP)大鼠背根神经节(dorsal root ganglia,DRG)Toll样受体4(toll-like receptor 4,TLR4)表达的影响. 方法 108只成年健康雄性SD大鼠,采用完全随机分组法分为4组:对照组(C组)、模型组(DNP组)、生理盐水(SC)+DNP组和GBP+DNP组(每组27只).观察链脲佐菌素(streptozocin,STZ)注射前1d及注射后3、7、10、14、21、28 d测定缩足反射机械刺激阈值(paw withdrawal mechanical threshold,PWMT)和缩足反射热辐射潜伏期(paw withdrawal thermal latency,PWTL),SC+DNP组和GBP+DNP组于STZ注射15d起分别腹腔注射生理盐水或GBP 50 mg/kg,1次/d,连续7d,并于21、28 d测定PWMT和PWTL.行为学测试完成后选择21 d大鼠用免疫组化和Western blot方法检测大鼠DRG中TLR4的表达. 结果 与C组比较,DNP组PWMT (3.9±0.9)g降低,PWTL[(6.1±0.8)s]缩短,DRG中TLR4蛋白的表达显著上调(P＜0.05)；与DNP组比较,SC+DNP组大鼠在腹腔注射生理盐水后,PWMT、PWTL和TLR4蛋白的表达无明显改变(P＞0.05)；与DNP组和SC+DNP组比较,GBP+DNP组PWMT(8.3±0.8)g升高,PWTL[(9.9±1.2)s]延长,DRG中TLR4蛋白的表达下调(P＜0.05). 结论 GBP可通过抑制DRG中TLR4表达,从而减轻大鼠DNP.
目的 探討加巴噴丁(gabapentin,GBP)對糖尿病神經病理性疼痛(diabetic neuropathic pain,DNP)大鼠揹根神經節(dorsal root ganglia,DRG)Toll樣受體4(toll-like receptor 4,TLR4)錶達的影響. 方法 108隻成年健康雄性SD大鼠,採用完全隨機分組法分為4組:對照組(C組)、模型組(DNP組)、生理鹽水(SC)+DNP組和GBP+DNP組(每組27隻).觀察鏈脲佐菌素(streptozocin,STZ)註射前1d及註射後3、7、10、14、21、28 d測定縮足反射機械刺激閾值(paw withdrawal mechanical threshold,PWMT)和縮足反射熱輻射潛伏期(paw withdrawal thermal latency,PWTL),SC+DNP組和GBP+DNP組于STZ註射15d起分彆腹腔註射生理鹽水或GBP 50 mg/kg,1次/d,連續7d,併于21、28 d測定PWMT和PWTL.行為學測試完成後選擇21 d大鼠用免疫組化和Western blot方法檢測大鼠DRG中TLR4的錶達. 結果 與C組比較,DNP組PWMT (3.9±0.9)g降低,PWTL[(6.1±0.8)s]縮短,DRG中TLR4蛋白的錶達顯著上調(P＜0.05)；與DNP組比較,SC+DNP組大鼠在腹腔註射生理鹽水後,PWMT、PWTL和TLR4蛋白的錶達無明顯改變(P＞0.05)；與DNP組和SC+DNP組比較,GBP+DNP組PWMT(8.3±0.8)g升高,PWTL[(9.9±1.2)s]延長,DRG中TLR4蛋白的錶達下調(P＜0.05). 結論 GBP可通過抑製DRG中TLR4錶達,從而減輕大鼠DNP.
목적 탐토가파분정(gabapentin,GBP)대당뇨병신경병이성동통(diabetic neuropathic pain,DNP)대서배근신경절(dorsal root ganglia,DRG)Toll양수체4(toll-like receptor 4,TLR4)표체적영향. 방법 108지성년건강웅성SD대서,채용완전수궤분조법분위4조:대조조(C조)、모형조(DNP조)、생리염수(SC)+DNP조화GBP+DNP조(매조27지).관찰련뇨좌균소(streptozocin,STZ)주사전1d급주사후3、7、10、14、21、28 d측정축족반사궤계자격역치(paw withdrawal mechanical threshold,PWMT)화축족반사열복사잠복기(paw withdrawal thermal latency,PWTL),SC+DNP조화GBP+DNP조우STZ주사15d기분별복강주사생리염수혹GBP 50 mg/kg,1차/d,련속7d,병우21、28 d측정PWMT화PWTL.행위학측시완성후선택21 d대서용면역조화화Western blot방법검측대서DRG중TLR4적표체. 결과 여C조비교,DNP조PWMT (3.9±0.9)g강저,PWTL[(6.1±0.8)s]축단,DRG중TLR4단백적표체현저상조(P＜0.05)；여DNP조비교,SC+DNP조대서재복강주사생리염수후,PWMT、PWTL화TLR4단백적표체무명현개변(P＞0.05)；여DNP조화SC+DNP조비교,GBP+DNP조PWMT(8.3±0.8)g승고,PWTL[(9.9±1.2)s]연장,DRG중TLR4단백적표체하조(P＜0.05). 결론 GBP가통과억제DRG중TLR4표체,종이감경대서DNP.
Objective To investigate the effect of systemic administration of gabapentin(GBP) on the expression of toll-like receptor 4(TLR4) in dorsal root ganglion neurons in a rat model of diabetic neuropathic pain (DNP).Methods One hundred and eight male SD rats weighing 200 g-220 g were randomly divided into 4 groups (n=27):control group (group C),DNP group,saline group (SC+DNP group)and gabapentin group(GBP+DNP group).Diabetes was induced with streptozocin(70 mg/kg) subcutaneously.Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured at 1 d before and 3,7,10,14,21,28 d after streptozocin injection and 21,28 d after administration of GBP (50 mg/kg,once)intraperitoneally.Saline and GBP (50 mg/kg) were given intraperitoneally once a day for 7 days starting from 15 d after streptozocin injection in saline and GBP groups respectively.The expression of TLR4 was determined at 21 d after streptozocin injection by immune.Histochemistry and Western blot.Results Compared with group C,PWMT (3.9 ±0.9) was significantly decreased,PWTL [(6.1 ±0.8) s]was significantly shorten,TLR4 in expression was significantly increased in DNP,SC+DNP group and GBP+DNP group (P＜0.05).Compared with SC+DNP group,PWMT(8.3±0.8)was significantly increased,PWTL[(9.9±1.2) s]was significantly prolonged,TLR4 expression was significantly decreased in GBP+DNP group (P＜0.05).There was no significant difference in the parameters mentioned above between DNP and SC+DNP group (P＞0.05).Conclusions Systemic administration of GBP attenuates diabetic neuropathic pain by inhibiting the expression of TLR4 in DRG neurons.
