国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2014年
2期
134-137,144
,共5页
张兢%顾小萍%程崇学%马正良
張兢%顧小萍%程崇學%馬正良
장긍%고소평%정숭학%마정량
钙-钙调蛋白依赖性激酶Ⅱ%N-甲基-D-天冬氨酸受体2B亚型%瑞芬太尼%痛觉过敏%切口痛
鈣-鈣調蛋白依賴性激酶Ⅱ%N-甲基-D-天鼕氨痠受體2B亞型%瑞芬太尼%痛覺過敏%切口痛
개-개조단백의뢰성격매Ⅱ%N-갑기-D-천동안산수체2B아형%서분태니%통각과민%절구통
Ca2+/calmodulin dependent protein kinase Ⅱ%N-methyl-D-aspartate receptor 2B%Remifentanil%Hyperalgesia%Incision pain
目的 观察分别鞘内注射钙钙调蛋白依赖性蛋白激酶Ⅱ(Ca2+/calmodulin dependent protein kinaseⅡ,CaMKⅡ)高效特异性抑制剂KN93或艾芬地尔(Ifenprodil)对瑞芬太尼诱发切口痛大鼠痛觉过敏的镇痛作用.方法 SD雄性大鼠30只,按随机数字表法分为5组(每组6只):空白对照组(C组)、切口痛(Ⅰ组)、瑞芬太尼组(R组)、KN93组(K组)及Ifenprodil组(F组).除C组外均做切口痛模型,R组、K组及F组制作模型的同时经皮下泵注瑞芬太尼(0.04 mg/kg)30 min注完.R组、K组及F组术前30 min分别鞘内给予10％二甲基亚砜(dimethyl sulfoxide,DMSO)20μ1、KN93 20μl(50 μg/10μl)和20μlIfenprodil(10 μg/20μl).各组分别在术前24h及术后2、6、24、48 h检测术侧热缩足潜伏期(paw withdrawal thermal latency,PWTL)及机械缩足阈值(paw withdrawal mechanical threshold,PMWT).结果 与C组比较,Ⅰ组术后手术侧PWTL及PMWT明显降低；与Ⅰ组比较,R组术后手术侧PWTL及PMWT明显降低；与R组比较,K组术后各时间点PWTL (15.32±0.73)、(15.79±0.32)、(14.86±0.98)、(14.76±0.82)s及PMWT (31.8±1.4)、(29.3±1.2)、(30.8±1.0)、(30.5±1.2)g均明显升高(P＜0.05),F组术后各时间点PWTL(14.24±0.79)、(15.33-0.29)、(15.29±-0.74)、(15.21±-0.45)s及PMWT(29.3±1.8)、(30.2±2.1)、(29.5±1.5)、(30.2±1.0)g均明显升高(P＜0.05).结论 鞘内注射KN93或Ifenprodil均能够有效缓解瑞芬太尼诱发的痛觉过敏.
目的 觀察分彆鞘內註射鈣鈣調蛋白依賴性蛋白激酶Ⅱ(Ca2+/calmodulin dependent protein kinaseⅡ,CaMKⅡ)高效特異性抑製劑KN93或艾芬地爾(Ifenprodil)對瑞芬太尼誘髮切口痛大鼠痛覺過敏的鎮痛作用.方法 SD雄性大鼠30隻,按隨機數字錶法分為5組(每組6隻):空白對照組(C組)、切口痛(Ⅰ組)、瑞芬太尼組(R組)、KN93組(K組)及Ifenprodil組(F組).除C組外均做切口痛模型,R組、K組及F組製作模型的同時經皮下泵註瑞芬太尼(0.04 mg/kg)30 min註完.R組、K組及F組術前30 min分彆鞘內給予10％二甲基亞砜(dimethyl sulfoxide,DMSO)20μ1、KN93 20μl(50 μg/10μl)和20μlIfenprodil(10 μg/20μl).各組分彆在術前24h及術後2、6、24、48 h檢測術側熱縮足潛伏期(paw withdrawal thermal latency,PWTL)及機械縮足閾值(paw withdrawal mechanical threshold,PMWT).結果 與C組比較,Ⅰ組術後手術側PWTL及PMWT明顯降低；與Ⅰ組比較,R組術後手術側PWTL及PMWT明顯降低；與R組比較,K組術後各時間點PWTL (15.32±0.73)、(15.79±0.32)、(14.86±0.98)、(14.76±0.82)s及PMWT (31.8±1.4)、(29.3±1.2)、(30.8±1.0)、(30.5±1.2)g均明顯升高(P＜0.05),F組術後各時間點PWTL(14.24±0.79)、(15.33-0.29)、(15.29±-0.74)、(15.21±-0.45)s及PMWT(29.3±1.8)、(30.2±2.1)、(29.5±1.5)、(30.2±1.0)g均明顯升高(P＜0.05).結論 鞘內註射KN93或Ifenprodil均能夠有效緩解瑞芬太尼誘髮的痛覺過敏.
목적 관찰분별초내주사개개조단백의뢰성단백격매Ⅱ(Ca2+/calmodulin dependent protein kinaseⅡ,CaMKⅡ)고효특이성억제제KN93혹애분지이(Ifenprodil)대서분태니유발절구통대서통각과민적진통작용.방법 SD웅성대서30지,안수궤수자표법분위5조(매조6지):공백대조조(C조)、절구통(Ⅰ조)、서분태니조(R조)、KN93조(K조)급Ifenprodil조(F조).제C조외균주절구통모형,R조、K조급F조제작모형적동시경피하빙주서분태니(0.04 mg/kg)30 min주완.R조、K조급F조술전30 min분별초내급여10％이갑기아풍(dimethyl sulfoxide,DMSO)20μ1、KN93 20μl(50 μg/10μl)화20μlIfenprodil(10 μg/20μl).각조분별재술전24h급술후2、6、24、48 h검측술측열축족잠복기(paw withdrawal thermal latency,PWTL)급궤계축족역치(paw withdrawal mechanical threshold,PMWT).결과 여C조비교,Ⅰ조술후수술측PWTL급PMWT명현강저；여Ⅰ조비교,R조술후수술측PWTL급PMWT명현강저；여R조비교,K조술후각시간점PWTL (15.32±0.73)、(15.79±0.32)、(14.86±0.98)、(14.76±0.82)s급PMWT (31.8±1.4)、(29.3±1.2)、(30.8±1.0)、(30.5±1.2)g균명현승고(P＜0.05),F조술후각시간점PWTL(14.24±0.79)、(15.33-0.29)、(15.29±-0.74)、(15.21±-0.45)s급PMWT(29.3±1.8)、(30.2±2.1)、(29.5±1.5)、(30.2±1.0)g균명현승고(P＜0.05).결론 초내주사KN93혹Ifenprodil균능구유효완해서분태니유발적통각과민.
Objective To investigate the preventive effect of intrathecal KN93 or Ifenprodil administration on remifentanil-induced incisional hyperalgesiaafter surgery iincisional pain in rats.Methods Thirty SD rats were randomly divided into 5 groups (n=6):control group (C group),incision pain group (Ⅰ group),Remifentanil group (R group),KN93 group (K group) and Ifenprodil group(F group).A hind paw incisional model was used to induce incisional painexcept for control group.At the same time,0.04 mg/kg remifentanil was infused subcutaneously 30 min in R group,K group and F group,30 min before incisional model,20 μl dimethyl sulfoxide (DMSO,10％),20 μl KN93 (50 μg/10 μl) and 20 μl Ifenprodil (10 μg/20 μl) were intrathecally injected to rats in R group,K group or F group respectively.Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were tested on 24 h before and 2,6,24,48 h after operation.Results The PWTL and PMWT on the ipsilateral side significantly decreased after operation in Ⅰ group compared with C group; and evenshortenin R group than that in Ⅰ group.Compared with R group,the PWTL(15.32±0.73),(15.79±0.32),(14.86±0.98),(14.76±0.82) s and PMWT (31.8±1.4),(29.3±1.2),(30.8±1.0),(30.5±1.2) g significantly increased in K group(P＜0.05) at each time point.Meanwhile,the PWTL(14.24±0.79),(15.33±0.29),(15.29±0.74),(15.21±0.45) s and PMWT(29.3±1.8),(30.2±2.1),(29.5±1.5),(30.2±1.0) g obviously increased in F group(P＜0.05) at each time point.Conclusions Intrathecal injection of KN93 or Ifenprodil coulde effectively alleviate remifentanil-induced hyperalgesia.
