国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2014年
5期
423-425,440
,共4页
血管性痴呆%老年%异氟烷%学习记忆%乙酰胆碱%乙酰胆碱转移酶%乙酰胆碱酯酶%海马
血管性癡呆%老年%異氟烷%學習記憶%乙酰膽堿%乙酰膽堿轉移酶%乙酰膽堿酯酶%海馬
혈관성치태%노년%이불완%학습기억%을선담감%을선담감전이매%을선담감지매%해마
Vascular dementia%Old age%Isoflurane%Learning and memory%Acetylcholine%Choline acetyltransferase%Acetylcholinesterase%Hippocampus
目的 研究异氟醚对血管性痴呆(vascular dementia,VaD)老年大鼠海马中胆碱能系统的影响. 方法 采用四血管法(4-vessel occlusion,4-VO)制作VaD老年(15月龄)大鼠模型,选用60只SD老年大鼠,雌雄各半,制作成VaD老年大鼠.根据异氟烷吸入浓度(0、1.5％、2.0％)和吸入后开始测试胆碱能递质的天数(1d和7d)将上述大鼠分为6组,即:1 d未吸入异氟烷组(Ⅰ1.0),1d吸入1.5％异氟烷组(Ⅰ1,15％),1d吸入2.0％异氟烷组(Ⅰ12.0％),7d未吸入异氟烷组(Ⅰ7.0),7d吸入1.5％异氟烷组(Ⅰ7.1.5％),7d吸入2.0％异氟烷组(Ⅰ7,2.0％).吸入异氟烷后在第1天和第7天分别进行Morris水迷宫测试.水迷宫测试完成后,立即处死大鼠,在冰面取出大鼠的海马,放入-80℃的冰箱中保存.然后检测海马中的乙酰胆碱含量、乙酰胆碱转移酶及乙酰胆碱酯酶的活力. 结果 吸入1.5％和2.0％异氟烷1d后,海马中乙酰胆碱含量[(82±10) μg/g和(65±13)μg/g]和乙酰胆碱转移酶活力[(98±11) IU/g和(81±13) IU/g]均明显低于未吸入异氟烷组[乙酰胆碱含量(99±13) μg/g和乙酰胆碱转移酶(115±11)IU/g],吸入2.0％异氟烷模型大鼠下降更为明显(P＜0.05);随着吸入异氟烷浓度不同而乙酰胆碱酯酶[(0.75±0.33)、(0.85±0.35) U/mgprot和(0.55±0.22) U/mgprot]的变化差异无统计学意义(P＞0.05).模型大鼠吸入异氟烷后第7天,各组大鼠海马中乙酰胆碱含量[(99±13)、(97±13)μg/g和(98±12) μg/g]和乙酰胆碱转移酶[(118±12)、(128±15)IU/g和(116±15) IU/g]及乙酰胆碱酯酶活力[(0.71±0.29)、(0.70±0.11) U/mgprot和(0.66±0.38) U/mgprot]没有明显变化(P＞0.05). 结论 异氟烷能使VaD老年大鼠海马中乙酰胆碱含量及乙酰胆碱转移酶活力短暂下降,而对乙酰胆碱酯酶无明显影响.
目的 研究異氟醚對血管性癡呆(vascular dementia,VaD)老年大鼠海馬中膽堿能繫統的影響. 方法 採用四血管法(4-vessel occlusion,4-VO)製作VaD老年(15月齡)大鼠模型,選用60隻SD老年大鼠,雌雄各半,製作成VaD老年大鼠.根據異氟烷吸入濃度(0、1.5％、2.0％)和吸入後開始測試膽堿能遞質的天數(1d和7d)將上述大鼠分為6組,即:1 d未吸入異氟烷組(Ⅰ1.0),1d吸入1.5％異氟烷組(Ⅰ1,15％),1d吸入2.0％異氟烷組(Ⅰ12.0％),7d未吸入異氟烷組(Ⅰ7.0),7d吸入1.5％異氟烷組(Ⅰ7.1.5％),7d吸入2.0％異氟烷組(Ⅰ7,2.0％).吸入異氟烷後在第1天和第7天分彆進行Morris水迷宮測試.水迷宮測試完成後,立即處死大鼠,在冰麵取齣大鼠的海馬,放入-80℃的冰箱中保存.然後檢測海馬中的乙酰膽堿含量、乙酰膽堿轉移酶及乙酰膽堿酯酶的活力. 結果 吸入1.5％和2.0％異氟烷1d後,海馬中乙酰膽堿含量[(82±10) μg/g和(65±13)μg/g]和乙酰膽堿轉移酶活力[(98±11) IU/g和(81±13) IU/g]均明顯低于未吸入異氟烷組[乙酰膽堿含量(99±13) μg/g和乙酰膽堿轉移酶(115±11)IU/g],吸入2.0％異氟烷模型大鼠下降更為明顯(P＜0.05);隨著吸入異氟烷濃度不同而乙酰膽堿酯酶[(0.75±0.33)、(0.85±0.35) U/mgprot和(0.55±0.22) U/mgprot]的變化差異無統計學意義(P＞0.05).模型大鼠吸入異氟烷後第7天,各組大鼠海馬中乙酰膽堿含量[(99±13)、(97±13)μg/g和(98±12) μg/g]和乙酰膽堿轉移酶[(118±12)、(128±15)IU/g和(116±15) IU/g]及乙酰膽堿酯酶活力[(0.71±0.29)、(0.70±0.11) U/mgprot和(0.66±0.38) U/mgprot]沒有明顯變化(P＞0.05). 結論 異氟烷能使VaD老年大鼠海馬中乙酰膽堿含量及乙酰膽堿轉移酶活力短暫下降,而對乙酰膽堿酯酶無明顯影響.
목적 연구이불미대혈관성치태(vascular dementia,VaD)노년대서해마중담감능계통적영향. 방법 채용사혈관법(4-vessel occlusion,4-VO)제작VaD노년(15월령)대서모형,선용60지SD노년대서,자웅각반,제작성VaD노년대서.근거이불완흡입농도(0、1.5％、2.0％)화흡입후개시측시담감능체질적천수(1d화7d)장상술대서분위6조,즉:1 d미흡입이불완조(Ⅰ1.0),1d흡입1.5％이불완조(Ⅰ1,15％),1d흡입2.0％이불완조(Ⅰ12.0％),7d미흡입이불완조(Ⅰ7.0),7d흡입1.5％이불완조(Ⅰ7.1.5％),7d흡입2.0％이불완조(Ⅰ7,2.0％).흡입이불완후재제1천화제7천분별진행Morris수미궁측시.수미궁측시완성후,립즉처사대서,재빙면취출대서적해마,방입-80℃적빙상중보존.연후검측해마중적을선담감함량、을선담감전이매급을선담감지매적활력. 결과 흡입1.5％화2.0％이불완1d후,해마중을선담감함량[(82±10) μg/g화(65±13)μg/g]화을선담감전이매활력[(98±11) IU/g화(81±13) IU/g]균명현저우미흡입이불완조[을선담감함량(99±13) μg/g화을선담감전이매(115±11)IU/g],흡입2.0％이불완모형대서하강경위명현(P＜0.05);수착흡입이불완농도불동이을선담감지매[(0.75±0.33)、(0.85±0.35) U/mgprot화(0.55±0.22) U/mgprot]적변화차이무통계학의의(P＞0.05).모형대서흡입이불완후제7천,각조대서해마중을선담감함량[(99±13)、(97±13)μg/g화(98±12) μg/g]화을선담감전이매[(118±12)、(128±15)IU/g화(116±15) IU/g]급을선담감지매활력[(0.71±0.29)、(0.70±0.11) U/mgprot화(0.66±0.38) U/mgprot]몰유명현변화(P＞0.05). 결론 이불완능사VaD노년대서해마중을선담감함량급을선담감전이매활력단잠하강,이대을선담감지매무명현영향.
Objective To investigate the effects of isoflurane at different concentrations on the cholinergic system on the hippocampus of aged rats with vascular dementia(VaD).Methods The model of the old VaD rats was established with the method of 4-vessel occlusion(4-VO) in 60 aged SD rats (30 males and 30 females).The animals were divided into 6 groups according to the days(days 1 and 7)after isoflurane inhalation and the concertrations(0,1.5％ and 2.0％):1 d 0 isoflurane group(Ⅰ 1,0),1 d 1.5％ isoflurane group(Ⅰ 1,5％),1 d 2.0％ isoflurane group(Ⅰ 1,20％),7 d 0 isoflurane group(Ⅰ 7,0),7 d 1.5％ isoflurane group(Ⅰ 7,5％),7 d 2.0％ isoflurane group(Ⅰ 7,2.0％).On day 1 and day 7 after isoflurane inhalation,morris water maze testing was performed.After Morris water maze testing was completed,the aged rats with VaD were killed at once.Then the hippocampus of the aged rats with VaD were dislodged on ice and reserved in icebox at subzero 80 temperature with iquid nitrogen frozening in order to detect the content of acetylcholine,choline acetyltransferase and acetylcholinesterase.The content of acetylcholine in hippocampus is detected by Elisa method,and the content of choline acetyltransferase is detected by kit of choline acetyltransferase and the content of acetylcholinesterase is detected by kit of acetylcholinesterase.Results On the 1st day after isoflurane inhalation,the content of acetylcholine [Ⅰ 1,1.5％(82±10) μg/g and Ⅰ 1.2.0％(65±13) μg/g] and choline acetyltransferase [Ⅰ 1,1.5％(98±11) IU/g and Ⅰ 1,1.20％(81±13) IU/g] in hippocampus were also markedly lower than those of 0 isoflurane group[the content of acetylcholine (99±13) μg/g and the content of choline acetyltransferase (115±11) IU/g] (P＜0.05),of which the 2.0％ isoflurane group had the lowest(P＜0.05).There were not significant different of acetylcholinesterase with different concertrations of isoflurane inhalation (P＞0.05).On the 7th day after isoflurane inhalations,no significant differences were observed the content of acetylcholine[(99±13),(97±13) μg/g and (98±12) μg/g],choline acetyltransferase [(118±12),(128±15) IU/g and (116±15) IU/g] and acetylcholinesterase [(0.71±0.29),(0.70±0.11) U/mgprot and (0.66±0.38) U/mgprot] in hippocampus among three groups (P＞0.05).Conclusions After VaD rats suck in isoflurane,the content of acetylcholine and choline acetyltransferase in the hippocampus were decreased but only temporary,but the contents of acetylcholinesterase was notinfluenced.
