国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2014年
6期
481-485
,共5页
脓毒症%B1细胞%IgM
膿毒癥%B1細胞%IgM
농독증%B1세포%IgM
Sepsis%B1 cells%IgM
目的 观察脓毒症时不同时间点不同部位B1细胞计数和分泌IgM能力的变化,以初步探索B1细胞在脓毒症中的作用. 方法 收集脓毒症患者和健康对照者外周血样本,流式细胞术检测两组人群外周血B细胞和B1细胞比例.取18只C57BL/6小鼠采用随机数字表法随机分为假手术组(Sham组)和盲肠结扎穿孔(cecal ligation and puncture,CLP)组,根据CLP的次数又进一步分为CLP-1组和CLP-2组,即分别穿刺1次和2次,每组6只.取外周血、脾脏和腹腔灌洗液,流式细胞术检测B细胞和B1细胞计数和百分比的变化,腹腔B1细胞分泌膜IgM的变化. 结果 ①流式细胞术检测结果显示脓毒症患者外周血B细胞和B1细胞百分比为(9±4)％和(18±8)％,低于健康对照者(16±6)％和(36±5)％(P＜0.05);②小鼠CLP-1组术后24 h,外周血中B细胞和B1细胞计数为(6.00±4.20)×102/ml和(3.86±2.88)×102/ml,显著低于Sham组(3.29±0.39)×103/ml和(2.04±0.28)×103/ml(P＜0.05);③CLP-1组小鼠术后24 h,脾脏中B细胞和B1细胞计数为(9.13±3.24)×105/ml和(2.08±0.71)×105/ml,显著低于Sham组(6.71±0.71)× 106/ml和(1.29±0.88)×106/ml(P＜0.05);④CLP-1组小鼠术后24 h和48 h腹腔中的B细胞百分比较Sham组降低(P＜0.05),而B1细胞在6h和24 h降低(P＜0.05),48 h无变化;⑤严重脓毒症时(即CLP-2组)小鼠B1细胞分泌IgM百分比为(61±7)％,较Sham组减少(84±6)％(P＜0.05). 结论 脓毒症患者和小鼠B1细胞的数量和功能均发生负向变化,提示B1细胞可能参与了脓毒症诱导的免疫功能障碍.
目的 觀察膿毒癥時不同時間點不同部位B1細胞計數和分泌IgM能力的變化,以初步探索B1細胞在膿毒癥中的作用. 方法 收集膿毒癥患者和健康對照者外週血樣本,流式細胞術檢測兩組人群外週血B細胞和B1細胞比例.取18隻C57BL/6小鼠採用隨機數字錶法隨機分為假手術組(Sham組)和盲腸結扎穿孔(cecal ligation and puncture,CLP)組,根據CLP的次數又進一步分為CLP-1組和CLP-2組,即分彆穿刺1次和2次,每組6隻.取外週血、脾髒和腹腔灌洗液,流式細胞術檢測B細胞和B1細胞計數和百分比的變化,腹腔B1細胞分泌膜IgM的變化. 結果 ①流式細胞術檢測結果顯示膿毒癥患者外週血B細胞和B1細胞百分比為(9±4)％和(18±8)％,低于健康對照者(16±6)％和(36±5)％(P＜0.05);②小鼠CLP-1組術後24 h,外週血中B細胞和B1細胞計數為(6.00±4.20)×102/ml和(3.86±2.88)×102/ml,顯著低于Sham組(3.29±0.39)×103/ml和(2.04±0.28)×103/ml(P＜0.05);③CLP-1組小鼠術後24 h,脾髒中B細胞和B1細胞計數為(9.13±3.24)×105/ml和(2.08±0.71)×105/ml,顯著低于Sham組(6.71±0.71)× 106/ml和(1.29±0.88)×106/ml(P＜0.05);④CLP-1組小鼠術後24 h和48 h腹腔中的B細胞百分比較Sham組降低(P＜0.05),而B1細胞在6h和24 h降低(P＜0.05),48 h無變化;⑤嚴重膿毒癥時(即CLP-2組)小鼠B1細胞分泌IgM百分比為(61±7)％,較Sham組減少(84±6)％(P＜0.05). 結論 膿毒癥患者和小鼠B1細胞的數量和功能均髮生負嚮變化,提示B1細胞可能參與瞭膿毒癥誘導的免疫功能障礙.
목적 관찰농독증시불동시간점불동부위B1세포계수화분비IgM능력적변화,이초보탐색B1세포재농독증중적작용. 방법 수집농독증환자화건강대조자외주혈양본,류식세포술검측량조인군외주혈B세포화B1세포비례.취18지C57BL/6소서채용수궤수자표법수궤분위가수술조(Sham조)화맹장결찰천공(cecal ligation and puncture,CLP)조,근거CLP적차수우진일보분위CLP-1조화CLP-2조,즉분별천자1차화2차,매조6지.취외주혈、비장화복강관세액,류식세포술검측B세포화B1세포계수화백분비적변화,복강B1세포분비막IgM적변화. 결과 ①류식세포술검측결과현시농독증환자외주혈B세포화B1세포백분비위(9±4)％화(18±8)％,저우건강대조자(16±6)％화(36±5)％(P＜0.05);②소서CLP-1조술후24 h,외주혈중B세포화B1세포계수위(6.00±4.20)×102/ml화(3.86±2.88)×102/ml,현저저우Sham조(3.29±0.39)×103/ml화(2.04±0.28)×103/ml(P＜0.05);③CLP-1조소서술후24 h,비장중B세포화B1세포계수위(9.13±3.24)×105/ml화(2.08±0.71)×105/ml,현저저우Sham조(6.71±0.71)× 106/ml화(1.29±0.88)×106/ml(P＜0.05);④CLP-1조소서술후24 h화48 h복강중적B세포백분비교Sham조강저(P＜0.05),이B1세포재6h화24 h강저(P＜0.05),48 h무변화;⑤엄중농독증시(즉CLP-2조)소서B1세포분비IgM백분비위(61±7)％,교Sham조감소(84±6)％(P＜0.05). 결론 농독증환자화소서B1세포적수량화공능균발생부향변화,제시B1세포가능삼여료농독증유도적면역공능장애.
Objective To investigate the change of expression on B1 cells in different sites at several times in sepsis and to explore the role of B1 cells in sepsis.Methods Blood samples were collected from sepsis patients and nomal persons,C57BL/6 mice were randomly divided into Sham group and cecal ligation and puncture (CLP) group,accroding to the times of puncture,CLP group was further divided to CLP-1 group and CLP-2 group,as puncture once and puncture twice respectively.Blood sample,spleen,and peritoneal lavage fluids were collected.The number and the percentage of B cells and B1 cells as well as the change of IgM which was secreted by B1 cells in abdominal cavity were measured by flow cytometry.Results ① The results of flow cytometry revealed that the percentage of B cells and B1 cells in the blood of patients in sepsis is (9±4)％ and (18±8)％,relatively lower than healthy volunteers (16±6)％ vs (36±5)％(P＜0.05).② In mice,the number of B cells and B1 cells in blood of CLP-1 group is (6.00±4.20)×102/ml and (3.86±2.88)×102/ml,significant lower than Sham group (3.29±0.39)×103/ml vs (2.04±0.28)×103/ml (P＜0.05).③ The number of B cells and B1 cells in spleen of CLP-1 group is (9.13±3.24)×l05/ml and (2.08±0.71)×l05/ml,significant lower than Sham group (6.71±0.71)×106/ml vs (1.29±0.88)×106/ml(P＜0.05).④ As to Sham group,the percentage of B cells in peritoneal cavity of CLP-1 group after CLP 24 h and 48 h in mice is decreased (P＜0.05).However,only at the timc of 6 h and 24 h,the percentage of B1 cells is declined(P＜0.05).5 The percentage of secretion of IgM in B1 cells is (61±7)％ when mice experienced severe sepsis in CLP-2 group,which is lower than Sham group(84±6)％(P＜0.05).Conclusions Both the number and the function of B1 cells have negative changcs,it's suggested that B1 cells may involved in immune dysfunction of sepsis.
