CAJ | 학술논문

背景肺泡上皮细胞(alveolar epithelial cells.AECs)或肺微血管内皮细胞(pulmonary microvaseular endothelial cells,PMECs)缺氧/复氧(hypoxia/reoxygenation,H/R)损伤模型是体外研究肺缺血/再灌注损伤(lung ischemia/reperfusion injury,LI/RI)机制及保护策略的模拟模型. 目的探讨模型的建立方法和建模过程中的相关问题,为有效建立该模型提供参考.内容从模型建立的一般程序、细胞种类的选择与培养、H/R的方法、损伤检测指标等方面,对近年来国内外肺细胞H/R损伤模型的研究与应用情况进行综述. 趋向关于肺细胞H/R损伤模型的建立,目前尚无统一方法,需进一步研究.
배경 폐포상피세포(alveolar epithelial cells.AECs)혹폐미혈관내피세포(pulmonary microvaseular endothelial cells,PMECs)결양/복양(hypoxia/reoxygenation,H/R)손상모형시체외연구폐결혈/재관주손상(lung ischemia/reperfusion injury,LI/RI)궤제급보호책략적모의모형. 목적 탐토모형적건립방법화건모과정중적상관문제,위유효건립해모형제공삼고.내용 종모형건립적일반정서、세포충류적선택여배양、H/R적방법、손상검측지표등방면,대근년래국내외폐세포H/R손상모형적연구여응용정황진행종술. 추향 관우폐세포H/R손상모형적건립,목전상무통일방법,수진일보연구.
Background Hypoxia/reoxygenation (H/R) injury of alveolar epithelial cells (AECs) or pulmonary microvascular endothelial cells (PMECs) is an in vitro simulated model for studying the mechanisms and protective strategies of lung ischemia/reperfusion injury (LI/RI).Objective To explore the methods of establishing in vitro lung ischemia/reperfusion injury,models and related modeling issues,and to provide reference for effective:nodeling.Content Review of the research and application of lung iscbenfia/reperfusion injury models,including the general procedure of modeling,cell category and selection,methods of H/R,evaluation parameters of injury,etc.Trend There is no a uniform method to model pulmonary cell H/R injury so far.further research is needed.