国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2014年
9期
778-782
,共5页
关睿聪%肖金仿%于永群%瞿波%肖宁新
關睿聰%肖金倣%于永群%瞿波%肖寧新
관예총%초금방%우영군%구파%초저신
呛咳反应%辣椒素%脑损伤%S100B%细胞间黏附因子-1
嗆咳反應%辣椒素%腦損傷%S100B%細胞間黏附因子-1
창해반응%랄초소%뇌손상%S100B%세포간점부인자-1
Evoked cough%Capsaicin%Brain injury%S100B%Intercellular adhesion molecule-1
目的 构建颅脑术后呛咳模型,探讨犬颅脑手术后苏醒期呛咳与脑损伤因子S100B、细胞间黏附因子-1(intercellular adhesion molecule-1,ICAM-1)的关系. 方法 按完全随机分组法将24只家犬分为实验组和对照组(每组12只),24只家犬行颅脑手术,恢复自主呼吸时,实验组吸入辣椒素,对照组追加肌松药后再吸入辣椒素.辣椒素溶液定量雾化浓度为1 000 μmol/L,吸入时间为20 s.记录术前1 h(T0)、吸入雾化液时(T1)、吸入雾化液后1 min(T2)、吸入雾化液后5 min(T3)的平均动脉压(mean arterial blood pressure,MAP)和颅内压(intracranial pressure,ICP)改变.24 h后处死家犬,开颅取大小一致脑皮层组织,光镜观察脑出血情况,免疫组化S-P法检测S100B、ICAM-1在两组动物脑皮层组织中的表达情况. 结果 T2时实验组MAP[(153.1±8.1) mmHg(1 mmHg=0.133 kPa)]及ICP [(24.8±8.9) mmHg]与对照组MAP[(121.0±2.3) mmHg]及ICP[(12.6±1.2) mmHg]比较,差异有统计学意义(P＜0.01).免疫组织化学显示S100B弱阳性(+)中对照组7例、实验组0例,强阳性(+++)中对照组2例、实验组8例,ICAM-1阴性(-)中对照组6例、实验组1例,弱阳性(+)中对照组4例、实验组5例,中度阳性(++)中对照组2例、实验组4例,强阳性(+++)中对照组0例、实验组1例.S100B、ICAM-1在呛咳后实验组脑组织中过度表达,经Mann-Whitney U检验,实验组与对照组之间差异均有统计学意义. 结论 家犬雾化吸入辣椒素诱发呛咳反射可以作为研究呛咳反射的动物模型.S100B、ICAM-1在颅脑术后苏醒期呛咳表达增加.
目的 構建顱腦術後嗆咳模型,探討犬顱腦手術後囌醒期嗆咳與腦損傷因子S100B、細胞間黏附因子-1(intercellular adhesion molecule-1,ICAM-1)的關繫. 方法 按完全隨機分組法將24隻傢犬分為實驗組和對照組(每組12隻),24隻傢犬行顱腦手術,恢複自主呼吸時,實驗組吸入辣椒素,對照組追加肌鬆藥後再吸入辣椒素.辣椒素溶液定量霧化濃度為1 000 μmol/L,吸入時間為20 s.記錄術前1 h(T0)、吸入霧化液時(T1)、吸入霧化液後1 min(T2)、吸入霧化液後5 min(T3)的平均動脈壓(mean arterial blood pressure,MAP)和顱內壓(intracranial pressure,ICP)改變.24 h後處死傢犬,開顱取大小一緻腦皮層組織,光鏡觀察腦齣血情況,免疫組化S-P法檢測S100B、ICAM-1在兩組動物腦皮層組織中的錶達情況. 結果 T2時實驗組MAP[(153.1±8.1) mmHg(1 mmHg=0.133 kPa)]及ICP [(24.8±8.9) mmHg]與對照組MAP[(121.0±2.3) mmHg]及ICP[(12.6±1.2) mmHg]比較,差異有統計學意義(P＜0.01).免疫組織化學顯示S100B弱暘性(+)中對照組7例、實驗組0例,彊暘性(+++)中對照組2例、實驗組8例,ICAM-1陰性(-)中對照組6例、實驗組1例,弱暘性(+)中對照組4例、實驗組5例,中度暘性(++)中對照組2例、實驗組4例,彊暘性(+++)中對照組0例、實驗組1例.S100B、ICAM-1在嗆咳後實驗組腦組織中過度錶達,經Mann-Whitney U檢驗,實驗組與對照組之間差異均有統計學意義. 結論 傢犬霧化吸入辣椒素誘髮嗆咳反射可以作為研究嗆咳反射的動物模型.S100B、ICAM-1在顱腦術後囌醒期嗆咳錶達增加.
목적 구건로뇌술후창해모형,탐토견로뇌수술후소성기창해여뇌손상인자S100B、세포간점부인자-1(intercellular adhesion molecule-1,ICAM-1)적관계. 방법 안완전수궤분조법장24지가견분위실험조화대조조(매조12지),24지가견행로뇌수술,회복자주호흡시,실험조흡입랄초소,대조조추가기송약후재흡입랄초소.랄초소용액정량무화농도위1 000 μmol/L,흡입시간위20 s.기록술전1 h(T0)、흡입무화액시(T1)、흡입무화액후1 min(T2)、흡입무화액후5 min(T3)적평균동맥압(mean arterial blood pressure,MAP)화로내압(intracranial pressure,ICP)개변.24 h후처사가견,개로취대소일치뇌피층조직,광경관찰뇌출혈정황,면역조화S-P법검측S100B、ICAM-1재량조동물뇌피층조직중적표체정황. 결과 T2시실험조MAP[(153.1±8.1) mmHg(1 mmHg=0.133 kPa)]급ICP [(24.8±8.9) mmHg]여대조조MAP[(121.0±2.3) mmHg]급ICP[(12.6±1.2) mmHg]비교,차이유통계학의의(P＜0.01).면역조직화학현시S100B약양성(+)중대조조7례、실험조0례,강양성(+++)중대조조2례、실험조8례,ICAM-1음성(-)중대조조6례、실험조1례,약양성(+)중대조조4례、실험조5례,중도양성(++)중대조조2례、실험조4례,강양성(+++)중대조조0례、실험조1례.S100B、ICAM-1재창해후실험조뇌조직중과도표체,경Mann-Whitney U검험,실험조여대조조지간차이균유통계학의의. 결론 가견무화흡입랄초소유발창해반사가이작위연구창해반사적동물모형.S100B、ICAM-1재로뇌술후소성기창해표체증가.
Objective To investigate S100B,intercellular adhesion molecule-1 (ICAM-1) expression in craniotomy dogs associated reflex cough.Methods Twenty four dogs were given tracheal Intubation and craniotomy after general anesthesia.Dogs were randomly assigned to two groups:the experimental group (n=12) or normal group (n=12).Capsaicin (1 000 μmol/L) was delivered via dosimeter and one-way (inspiratory) valve attached to a side port between the two groups tracheal tubes and pneumotachograph.Cisatracurium was injected to the normal group at the same time.Reflex cough response in experimental group is equal or greater than normal group times.Extubation of two group was done 1 h postoperatively.The changes on mean arterial pressure (MAP) and intracranial pressure (ICP) were observed 1 h (T0) preoperatively,after inhalation of atomized liquid (T1),1 (T2),5 min (T3) after inhalation of atomized liquid.All dogs were executed 24 h postoperatively.After that,the staining slices of brain tissues with the same size were made,and the levels of S100B,ICAM-1 in dog's brain were analyzed by immunohistochemical method.Results The MAP and ICP in experimental group were significantly higher than those in control group [(153.1 ±8.1)mmHg (1 mmHg=0.133 kPa) vs (121.0±2.3) mmHg,and (24.8±8.9) mmHg vs (12.6±1.2) mmHg](P＜0.01).S100B was weakly positive (+) in the control group than in the experimental group (7 vs 0 cases).Two cases showed strong positive (+++) in the control group while 8 cases were present in the experimental group.Six cases showed ICAM-1 negative(-) in the control group,while only one in experimental group showed ICAM-1 negative.Four cases were weakly positive (+) in the control group,while 5 cases in experimental group.Two cases showed moderate positive (++) in the control group,and 4 cases in the experimental group.For strong positive,0 case was in control group (+++),while 1 case was in experimental group.The expression of S100B and ICAM-1 were significantly increased in the experimental groups.Mann-Whitney U analysis demonstrated that S100B and ICAM-1 expressions were associated with reflex cough.Conclusions The expressions on S100B,ICAM-1 are closely associated with reflex cough which may further exploded the blood vessel and injury the neurogliocyte cells.
